##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AgeI ApaI
AscI Asp700I Asp718I BamHI BciVI
BsiEI BsiWI BspDI BspHI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
FseI HincII HindII HpaI KpnI
KspI MfeI MluI MunI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI Sse8387I StuI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 2112 GTCTAC GT^[AC][GT]AC
AccIII 780 TCCGGA T^CCGGA
AhdI 4999 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 833 GTGCAC G^TGCAC
Aor51HI 3518 AGCGCT AGC^GCT
ApaLI 833 GTGCAC G^TGCAC
AseI 1298 ATTAAT AT^TAAT
AsnI 1298 ATTAAT AT^TAAT
AspEI 4999 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5077 TGCGCA TGC^GCA
AvrII 3629 CCTAGG C^CTAGG
BbeI 3047 GGCGCC GGCGC^C
BcgI 1956 CGATTAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 765 TGATCA T^GATCA
BlnI 3629 CCTAGG C^CTAGG
BsaBI 632 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 780 TCCGGA T^CCGGA
BsmBI 3317 CGTCTC CGTCTC(1/5)
Bsp1407I 4014 TGTACA T^GTACA
BspEI 780 TCCGGA T^CCGGA
BsrGI 4014 TGTACA T^GTACA
Eam1105I 4999 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3518 AGCGCT AGC^GCT
EcoRV 4362 GATATC GAT^ATC
FbaI 765 TGATCA T^GATCA
FspI 5077 TGCGCA TGC^GCA
HgaI 3611 GACGC GACGC(5/10)
HindIII 2434 AAGCTT A^AGCTT
KasI 3047 GGCGCC G^GCGCC
MamI 632 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 780 TCCGGA T^CCGGA
NaeI 4230 GCCGGC GCC^GGC
NarI 3047 GGCGCC GG^CGCC
NgoMIV 4230 GCCGGC G^CCGGC
NheI 1519 GCTAGC G^CTAGC
PpuMI 4980 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 5325 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1298 ATTAAT AT^TAAT
SacI 4493 GAGCTC GAGCT^C
SapI 4490 GAAGAGC GCTCTTC(1/4)
SfoI 3047 GGCGCC GGC^GCC
SmaI 492 CCCGGG CCC^GGG
SpeI 1083 ACTAGT A^CTAGT
SphI 2150 GCATGC GCATG^C
SspBI 4014 TGTACA T^GTACA
XmaCI 492 CCCGGG C^CCGGG
XmaI 492 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 3047 GGCGCC G[GA]^CG[CT]C
3611 GACGCC
AocI 2062 CCTGAGG CC^T[ACGT]AGG
4292 CCTCAGG
AspI 484 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3166 GACTCTGTC
BglI 75 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4291 GCCTCAGGGGC
BlpI 950 GCTAAGC GC^T[ACGT]AGC
5199 GCTGAGC
Bpu1102I 950 GCTAAGC GC^T[ACGT]AGC
5199 GCTGAGC
BsaHI 3047 GGCGCC G[GA]^CG[CT]C
3611 GACGCC
BseRI 2364 CTCCTC GAGGAG(10/8)
5509 CTCCTC
BsmI 4057 GAATGC GAATGC(1/-1)
1309 GCATTC
BspLU11I 1871 ACATGT A^CATGT
4046 ACATGT
BspMI 3659 GCAGGT ACCTGC(4/8)
3850 GCAGGT
BsrBI 2316 GAGCGG CCGCTC(-3/-3)
3881 GAGCGG
BsrFI 4230 GCCGGC [GA]^CCGG[CT]
4993 GCCGGT
BstEII 3583 GGTGACC G^GT[ACGT]ACC
3662 GGTTACC
BstPI 3583 GGTGACC G^GT[ACGT]ACC
3662 GGTTACC
Bsu36I 2062 CCTGAGG CC^T[ACGT]AGG
4292 CCTCAGG
CelII 950 GCTAAGC GC^T[ACGT]AGC
5199 GCTGAGC
Cfr10I 4230 GCCGGC [GA]^CCGG[CT]
4993 GCCGGT
DraI 2475 TTTAAA TTT^AAA
3494 TTTAAA
DraII 3795 AGGCCCC [GA]G^G[ACGT]CC[CT]
4980 AGGACCC
Eco81I 2062 CCTGAGG CC^T[ACGT]AGG
4292 CCTCAGG
EcoNI 3624 CCTTTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4694 CCTCACAAAGG
EcoO109I 3795 AGGCCCC [GA]G^G[ACGT]CC[CT]
4980 AGGACCC
EcoO65I 3583 GGTGACC G^GT[ACGT]ACC
3662 GGTTACC
EcoRI 755 GAATTC G^AATTC
1182 GAATTC
HaeII 3047 GGCGCC [GA]GCGC^[CT]
3518 AGCGCT
Hin1I 3047 GGCGCC G[GA]^CG[CT]C
3611 GACGCC
NcoI 157 CCATGG C^CATGG
658 CCATGG
NdeI 1235 CATATG CA^TATG
1452 CATATG
PflFI 484 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3166 GACTCTGTC
SexAI 3666 ACCTGGT A^CC[AT]GGT
5126 ACCTGGT
SspI 260 AATATT AAT^ATT
1249 AATATT
Tth111I 484 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3166 GACTCTGTC
XbaI 1011 TCTAGA T^CTAGA
3241 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BfrI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraIII DsaI
EaeI EarI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PmaCI PmlI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII