##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I AviII AvrII BbrPI
BfrI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrFI BssHII BssSI BstUI
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoNI EcoRV FseI
FspI KpnI KspI MluI MroI
MvnI NaeI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmlI PshBI PvuI RcaI
RsrII SacII SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI Sse8387I
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 4494 AACGTT AA^CGTT
AhdI 407 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1497 GTGCAC G^TGCAC
AocI 86 CCTGAGG CC^T[ACGT]AGG
ApaLI 1497 GTGCAC G^TGCAC
AspEI 407 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2105 CCCGAG C^[CT]CG[GA]G
BalI 4729 TGGCCA TGG^CCA
BamHI 2392 GGATCC G^GATCC
BbeI 4666 GGCGCC GGCGC^C
BcgI 4626 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 169 CCGGG CC^[GC]GG
BglI 1742 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 5075 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2105 CCCGAG C^[CT]CG[GA]G
BsrBI 300 CCGCTC CCGCTC(-3/-3)
Bst1107I 5231 GTATAC GTA^TAC
BstZ17I 5231 GTATAC GTA^TAC
Bsu36I 86 CCTGAGG CC^T[ACGT]AGG
DraIII 3414 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 407 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 86 CCTGAGG CC^T[ACGT]AGG
EcoRI 5561 GAATTC G^AATTC
EcoT22I 4997 ATGCAT ATGCA^T
HaeII 4666 GGCGCC [GA]GCGC^[CT]
HapII 169 CCGG C^CGG
HgaI 175 GACGC GACGC(5/10)
HpaII 169 CCGG C^CGG
KasI 4666 GGCGCC G^GCGCC
MamI 5075 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2478 CAATTG C^AATTG
MluNI 4729 TGGCCA TGG^CCA
MscI 4729 TGGCCA TGG^CCA
MspI 169 CCGG C^CGG
MunI 2478 CAATTG C^AATTG
NarI 4666 GGCGCC GG^CGCC
NciI 169 CCGGG CC^[GC]GG
NcoI 5752 CCATGG C^CATGG
NsiI 4997 ATGCAT ATGCA^T
PmeI 3549 GTTTAAAC GTTT^AAAC
PshAI 2182 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4494 AACGTT AA^CGTT
SacI 3251 GAGCTC GAGCT^C
SalI 3330 GTCGAC G^TCGAC
SfoI 4666 GGCGCC GGC^GCC
SphI 2913 GCATGC GCATG^C
StuI 2400 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 175 GACGCC G[GA]^CG[CT]C
4666 GGCGCC
AspI 1234 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1360 GACTCAGTC
BanI 1621 GGCACC G^G[CT][GA]CC
4666 GGCGCC
BclI 340 TGATCA T^GATCA
1822 TGATCA
BglII 3338 AGATCT A^GATCT
5361 AGATCT
BlpI 302 GCTCAGC GC^T[ACGT]AGC
4228 GCTCAGC
Bpu1102I 302 GCTCAGC GC^T[ACGT]AGC
4228 GCTCAGC
BsaHI 175 GACGCC G[GA]^CG[CT]C
4666 GGCGCC
BspMI 2722 ACCTGC ACCTGC(4/8)
3316 ACCTGC
BstBI 807 TTCGAA TT^CGAA
3357 TTCGAA
BstEII 1021 GGTCACC G^GT[ACGT]ACC
5527 GGTTACC
BstPI 1021 GGTCACC G^GT[ACGT]ACC
5527 GGTTACC
CelII 302 GCTCAGC GC^T[ACGT]AGC
4228 GCTCAGC
DraII 732 AGGTCCC [GA]G^G[ACGT]CC[CT]
2316 GGGACCT
DrdI 2503 GACACGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5626 GACTTTAAGGTC
DsaI 3670 CCGTGG C^C[GA][CT]GG
5752 CCATGG
EaeI 1405 TGGCCG [CT]^GGCC[GA]
4729 TGGCCA
EcoO109I 732 AGGTCCC [GA]G^G[ACGT]CC[CT]
2316 GGGACCT
EcoO65I 1021 GGTCACC G^GT[ACGT]ACC
5527 GGTTACC
EcoT14I 679 CCTTGG C^C[AT][AT]GG
5752 CCATGG
FbaI 340 TGATCA T^GATCA
1822 TGATCA
Hin1I 175 GACGCC G[GA]^CG[CT]C
4666 GGCGCC
HpaI 5494 GTTAAC GTT^AAC
5712 GTTAAC
NspV 807 TTCGAA TT^CGAA
3357 TTCGAA
PflFI 1234 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1360 GACTCAGTC
PpuMI 732 AGGTCCC [GA]G^G[AT]CC[CT]
2316 GGGACCT
SfuI 807 TTCGAA TT^CGAA
3357 TTCGAA
SmlI 1881 CTCAAG C^T[CT][GA]AG
4234 CTTGAG
SpeI 568 ACTAGT A^CTAGT
5676 ACTAGT
StyI 679 CCTTGG C^C[AT][AT]GG
5752 CCATGG
Tth111I 1234 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1360 GACTCAGTC
XcmI 1310 CCAGTTGGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1977 CCACTACTGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbsI BbvCI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI