##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AscI        
Asp718I     AvrII       BbeI        BbrPI       BbvCI       
BcgI        BlnI        BsiEI       BsiWI       BspDI       
BspLU11I    BssHII      BstBI       ClaI        CpoI        
EagI        EclXI       Eco52I      EcoT22I     FseI        
KasI        KpnI        MluI        NarI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SalI        SfiI        
SfoI        SfuI        SgrAI       SpeI        SphI        
StuI        SwaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       4208  TCCGGA               T^CCGGA
AflIII       6887  ACACGT               A^C[GA][CT]GT
AhdI         3993  GACCATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       6989  GTGCAC               G^TGCAC
Aor51HI      1649  AGCGCT               AGC^GCT
ApaLI        6989  GTGCAC               G^TGCAC
AseI         3199  ATTAAT               AT^TAAT
AsnI         3199  ATTAAT               AT^TAAT
AspEI        3993  GACCATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2825  TGCGCA               TGC^GCA
BclI         2321  TGATCA               T^GATCA
BlpI          112  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      112  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1720  TACGTA               [CT]AC^GT[GA]
BsaWI        4208  TCCGGA               [AT]^CCGG[AT]
BseAI        4208  TCCGGA               T^CCGGA
BsmBI        1272  GAGACG               CGTCTC(1/5)
Bsp1407I      684  TGTACA               T^GTACA
BspEI        4208  TCCGGA               T^CCGGA
BspHI        2913  TCATGA               T^CATGA
BsrGI         684  TGTACA               T^GTACA
Bst1107I     5517  GTATAC               GTA^TAC
BstEII       4740  GGTGACC              G^GT[ACGT]ACC
BstPI        4740  GGTGACC              G^GT[ACGT]ACC
BstZ17I      5517  GTATAC               GTA^TAC
CelII         112  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     3993  GACCATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1649  AGCGCT               AGC^GCT
EcoO65I      4740  GGTGACC              G^GT[ACGT]ACC
EcoRV        4186  GATATC               GAT^ATC
FbaI         2321  TGATCA               T^GATCA
FspI         2825  TGCGCA               TGC^GCA
HpaI         6339  GTTAAC               GTT^AAC
KspI          975  CCGCGG               CCGC^GG
MfeI         4553  CAATTG               C^AATTG
MroI         4208  TCCGGA               T^CCGGA
MunI         4553  CAATTG               C^AATTG
NaeI         2947  GCCGGC               GCC^GGC
NgoMIV       2947  GCCGGC               G^CCGGC
PshBI        3199  ATTAAT               AT^TAAT
RcaI         2913  TCATGA               T^CATGA
SacI         1519  GAGCTC               GAGCT^C
SacII         975  CCGCGG               CCGC^GG
ScaI         5087  AGTACT               AGT^ACT
SmaI         3179  CCCGGG               CCC^GGG
SnaBI        1720  TACGTA               TAC^GTA
SspBI         684  TGTACA               T^GTACA
SspI         4048  AATATT               AAT^ATT
XbaI         6718  TCTAGA               T^CTAGA
XmaCI        3179  CCCGGG               C^CCGGG
XmaI         3179  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         976  CGCG                 CG^CG
             3767  CGCG                
AcyI         2285  GACGCC               G[GA]^CG[CT]C
             4802  GGCGTC              
AflII          37  CTTAAG               C^TTAAG
             2626  CTTAAG              
AocI         4311  CCTGAGG              CC^T[ACGT]AGG
             7159  CCTGAGG             
AspI          672  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6046  GACCTCGTC           
BamHI        4205  GGATCC               G^GATCC
             6494  GGATCC              
BciVI        1631  GGATAC               GTATCC(6/5)
             5686  GTATCC              
BfrI           37  CTTAAG               C^TTAAG
             2626  CTTAAG              
BsaHI        2285  GACGCC               G[GA]^CG[CT]C
             4802  GGCGTC              
BsrBI        5883  CCGCTC               CCGCTC(-3/-3)
             3835  GAGCGG              
BstUI         976  CGCG                 CG^CG
             3767  CGCG                
BstXI         824  CCAGTGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4414  CCAACAACTTGG        
Bsu36I       4311  CCTGAGG              CC^T[ACGT]AGG
             7159  CCTGAGG             
DraIII       2464  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5635  CACGAGGTG           
Eco81I       4311  CCTGAGG              CC^T[ACGT]AGG
             7159  CCTGAGG             
HaeII        1649  AGCGCT               [GA]GCGC^[CT]
             5234  GGCGCT              
Hin1I        2285  GACGCC               G[GA]^CG[CT]C
             4802  GGCGTC              
HincII       4606  GTTGAC               GT[CT]^[GA]AC
             6339  GTTAAC              
HindII       4606  GTTGAC               GT[CT]^[GA]AC
             6339  GTTAAC              
MvnI          976  CGCG                 CG^CG
             3767  CGCG                
NdeI         3485  CATATG               CA^TATG
             4380  CATATG              
NspI          462  GCATGT               [GA]CATG^[CT]
             2792  ACATGC              
PaeR7I        995  CTCGAG               C^TCGAG
             1241  CTCGAG              
PflFI         672  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6046  GACCTCGTC           
SbfI         2925  CCTGCAGG             CCTGCA^GG
             3261  CCTGCAGG            
Sse8387I     2925  CCTGCAGG             CCTGCA^GG
             3261  CCTGCAGG            
Tth111I       672  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6046  GACCTCGTC           
XcmI         3365  CCAGCCTCAAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4414  CCAACAACTTGGTGG     
XhoI          995  CTCGAG               C^TCGAG
             1241  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BglII       BmyI        BpmI        BpuAI       
BsaBI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DrdI        DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmnI