##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AvaI AviII AvrII
BalI BanII BbeI BcgI BfrI
BlnI BsaHI BsiEI BsiWI BsoBI
BspDI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoT22I FauI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MluI MluNI MscI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 4509 TCCGGA T^CCGGA
AhdI 1710 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1856 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1710 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1589 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2821 CACGTG CAC^GTG
BciVI 3378 GGATAC GTATCC(6/5)
BglI 2634 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3298 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3298 GCTCAGC GC^T[ACGT]AGC
BsaAI 2821 CACGTG [CT]AC^GT[GA]
BsaI 2877 GGTCTC GGTCTC(1/5)
BseAI 4509 TCCGGA T^CCGGA
Bsp1407I 3948 TGTACA T^GTACA
BspEI 4509 TCCGGA T^CCGGA
BspHI 1989 TCATGA T^CATGA
BsrGI 3948 TGTACA T^GTACA
BstAPI 2509 GCAATTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3529 GGTTACC G^GT[ACGT]ACC
BstPI 3529 GGTTACC G^GT[ACGT]ACC
CelII 3298 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1710 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3327 GAAGAG CTCTTC(1/4)
EcoO65I 3529 GGTTACC G^GT[ACGT]ACC
HaeII 1213 GGCGCT [GA]GCGC^[CT]
Ksp632I 3327 GAAGAG CTCTTC(1/4)
MroI 4509 TCCGGA T^CCGGA
MspA1I 2235 CAGCTG C[AC]G^C[GT]G
NaeI 2762 GCCGGC GCC^GGC
NcoI 3650 CCATGG C^CATGG
NgoMIV 2762 GCCGGC G^CCGGC
PflFI 1589 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2821 CACGTG CAC^GTG
PmlI 2821 CACGTG CAC^GTG
PvuII 2235 CAGCTG CAG^CTG
RcaI 1989 TCATGA T^CATGA
SspBI 3948 TGTACA T^GTACA
Tth111I 1589 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccII 125 CGCG CG^CG
2563 CGCG
AclI 2101 AACGTT AA^CGTT
2308 AACGTT
AflIII 3224 ACATGT A^C[GA][CT]GT
4058 ACATGT
AocI 2501 CCTTAGG CC^T[ACGT]AGG
4694 CCTAAGG
AseI 775 ATTAAT AT^TAAT
5034 ATTAAT
AsnI 775 ATTAAT AT^TAAT
5034 ATTAAT
Asp700I 2265 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4725 GAACCCATTC
AspHI 2074 GTGCTC G[AT]GC[AT]^C
3697 GTGCTC
BamHI 1200 GGATCC G^GATCC
1503 GGATCC
BanI 82 GGCACC G^G[CT][GA]CC
2670 GGTGCC
BbsI 1611 GAAGAC GAAGAC(2/6)
3204 GTCTTC
BbvCI 2377 CCTCAGC CCTCAGC(-5/-2)
1696 GCTGAGG
BclI 716 TGATCA T^GATCA
1986 TGATCA
BcnI 1164 CCCGG CC^[GC]GG
2639 CCCGG
BmyI 2074 GTGCTC G[AGT]GC[ACT]^C
3697 GTGCTC
BpuAI 1611 GAAGAC GAAGAC(2/6)
3204 GTCTTC
BsaBI 1858 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4401 GATGACTATC
BsaWI 3313 TCCGGT [AT]^CCGG[AT]
4509 TCCGGA
BsiHKAI 2074 GTGCTC G[AT]GC[AT]^C
3697 GTGCTC
Bsp1286I 2074 GTGCTC G[AGT]GC[ACT]^C
3697 GTGCTC
BspLU11I 3224 ACATGT A^CATGT
4058 ACATGT
BspMI 2251 GCAGGT ACCTGC(4/8)
2874 GCAGGT
BsrFI 79 ACCGGC [GA]^CCGG[CT]
2762 GCCGGC
BstUI 125 CGCG CG^CG
2563 CGCG
Bsu36I 2501 CCTTAGG CC^T[ACGT]AGG
4694 CCTAAGG
Cfr10I 79 ACCGGC [GA]^CCGG[CT]
2762 GCCGGC
DraIII 218 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4118 CACAAAGTG
EaeI 2600 TGGCCG [CT]^GGCC[GA]
3006 TGGCCG
Eco81I 2501 CCTTAGG CC^T[ACGT]AGG
4694 CCTAAGG
EcoRI 3116 GAATTC G^AATTC
3244 GAATTC
EcoRV 822 GATATC GAT^ATC
1756 GATATC
FbaI 716 TGATCA T^GATCA
1986 TGATCA
MamI 1858 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4401 GATGACTATC
MvnI 125 CGCG CG^CG
2563 CGCG
NciI 1164 CCCGG CC^[GC]GG
2639 CCCGG
NdeI 1616 CATATG CA^TATG
1934 CATATG
PflMI 877 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1951 CCATCTAATGG
PpuMI 3155 GGGTCCT [GA]G^G[AT]CC[CT]
3504 GGGACCC
PshBI 775 ATTAAT AT^TAAT
5034 ATTAAT
Psp1406I 2101 AACGTT AA^CGTT
2308 AACGTT
SmlI 1364 CTCAAG C^T[CT][GA]AG
3286 CTTGAG
Van91I 877 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1951 CCATCTAATGG
XmnI 2265 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4725 GAACCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BglII
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII