################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI ApaI AscI Asp718I AspI AviII BbeI BbrPI BfrI BseAI BsiWI BspDI BspEI BsrBI BsrFI BssHII BstBI Cfr10I ClaI CpoI EcoRV FseI FspI HpaI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SspI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 1279 CGCG CG^CG AflIII 2796 ACATGT A^C[GA][CT]GT Alw44I 5335 GTGCAC G^TGCAC AocI 1441 CCTGAGG CC^T[ACGT]AGG Aor51HI 2931 AGCGCT AGC^GCT ApaLI 5335 GTGCAC G^TGCAC AseI 1343 ATTAAT AT^TAAT AsnI 1343 ATTAAT AT^TAAT Asp700I 90 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 3567 CTCGAG C^[CT]CG[GA]G BalI 3189 TGGCCA TGG^CCA BclI 2017 TGATCA T^GATCA BglII 4385 AGATCT A^GATCT BlpI 4736 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4736 GCTCAGC GC^T[ACGT]AGC BsaAI 2754 TACGTA [CT]AC^GT[GA] BsaBI 3476 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4908 GAGACC GGTCTC(1/5) BsiEI 2426 CGGCCG CG[GA][CT]^CG BsmI 3556 GAATGC GAATGC(1/-1) BsoBI 3567 CTCGAG C^[CT]CG[GA]G BspHI 2020 TCATGA T^CATGA BspLU11I 2796 ACATGT A^CATGT BspMI 574 GCAGGT ACCTGC(4/8) Bst1107I 1033 GTATAC GTA^TAC BstAPI 4392 GCATTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1738 GGTCACC G^GT[ACGT]ACC BstPI 1738 GGTCACC G^GT[ACGT]ACC BstUI 1279 CGCG CG^CG BstZ17I 1033 GTATAC GTA^TAC Bsu36I 1441 CCTGAGG CC^T[ACGT]AGG CelII 4736 GCTCAGC GC^T[ACGT]AGC DrdI 2472 GACTAAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2426 CGGCCG C^GGCCG EclXI 2426 CGGCCG C^GGCCG Eco47III 2931 AGCGCT AGC^GCT Eco52I 2426 CGGCCG C^GGCCG Eco81I 1441 CCTGAGG CC^T[ACGT]AGG EcoNI 3647 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1738 GGTCACC G^GT[ACGT]ACC FbaI 2017 TGATCA T^GATCA HaeII 2931 AGCGCT [GA]GCGC^[CT] HgaI 2479 GCGTC GACGC(5/10) HindIII 1242 AAGCTT A^AGCTT MamI 3476 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5554 CAATTG C^AATTG MluNI 3189 TGGCCA TGG^CCA MscI 3189 TGGCCA TGG^CCA MunI 5554 CAATTG C^AATTG MvnI 1279 CGCG CG^CG NheI 5104 GCTAGC G^CTAGC PaeR7I 3567 CTCGAG C^TCGAG PshBI 1343 ATTAAT AT^TAAT RcaI 2020 TCATGA T^CATGA SbfI 5115 CCTGCAGG CCTGCA^GG SnaBI 2754 TACGTA TAC^GTA SpeI 2386 ACTAGT A^CTAGT Sse8387I 5115 CCTGCAGG CCTGCA^GG StuI 5273 AGGCCT AGG^CCT SwaI 4626 ATTTAAAT ATTT^AAAT XbaI 2578 TCTAGA T^CTAGA XhoI 3567 CTCGAG C^TCGAG XmnI 90 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AatII 178 GACGTC GACGT^C 2883 GACGTC AcyI 178 GACGTC G[GA]^CG[CT]C 2883 GACGTC AhdI 5232 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5542 GACTTGTGGTC AspEI 5232 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5542 GACTTGTGGTC AvrII 5396 CCTAGG C^CTAGG 5468 CCTAGG BanI 3917 GGTGCC G^G[CT][GA]CC 5506 GGTGCC BbsI 4498 GAAGAC GAAGAC(2/6) 2353 GTCTTC BcnI 2346 CCCGG CC^[GC]GG 2424 CCCGG BglI 2413 GCCTTCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4555 GCCCTTCTGGC BlnI 5396 CCTAGG C^CTAGG 5468 CCTAGG BpuAI 4498 GAAGAC GAAGAC(2/6) 2353 GTCTTC BsaHI 178 GACGTC G[GA]^CG[CT]C 2883 GACGTC BsaWI 1431 ACCGGA [AT]^CCGG[AT] 1809 ACCGGA BsmBI 5707 CGTCTC CGTCTC(1/5) 2881 GAGACG BstXI 4456 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5510 CCAGAGGATTGG CfoI 1280 GCGC GCG^C 2932 GCGC EaeI 2426 CGGCCG [CT]^GGCC[GA] 3189 TGGCCA Eam1105I 5232 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5542 GACTTGTGGTC EcoRI 707 GAATTC G^AATTC 1153 GAATTC FauI 4975 CCCGC CCCGC(4/6) 2179 GCGGG HhaI 1280 GCGC GCG^C 2932 GCGC Hin1I 178 GACGTC G[GA]^CG[CT]C 2883 GACGTC HinP1I 1280 GCGC G^CGC 2932 GCGC MslI 76 CATAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3623 CATTTGGATG NciI 2346 CCCGG CC^[GC]GG 2424 CCCGG NcoI 3922 CCATGG C^CATGG 4224 CCATGG NdeI 296 CATATG CA^TATG 830 CATATG PpuMI 4134 AGGTCCT [GA]G^G[AT]CC[CT] 5121 GGGACCC SapI 1561 GCTCTTC GCTCTTC(1/4) 3033 GCTCTTC SmlI 3567 CTCGAG C^T[CT][GA]AG 4059 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BamHI BanII BbvCI BbvI BcgI BciVI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII