##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
ApaI AscI Asp718I AspI AviII
BbeI BbrPI BfrI BseAI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BstBI Cfr10I ClaI CpoI EcoRV
FseI FspI HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SspI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccII 1279 CGCG CG^CG
AflIII 2796 ACATGT A^C[GA][CT]GT
Alw44I 5335 GTGCAC G^TGCAC
AocI 1441 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2931 AGCGCT AGC^GCT
ApaLI 5335 GTGCAC G^TGCAC
AseI 1343 ATTAAT AT^TAAT
AsnI 1343 ATTAAT AT^TAAT
Asp700I 90 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3567 CTCGAG C^[CT]CG[GA]G
BalI 3189 TGGCCA TGG^CCA
BclI 2017 TGATCA T^GATCA
BglII 4385 AGATCT A^GATCT
BlpI 4736 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4736 GCTCAGC GC^T[ACGT]AGC
BsaAI 2754 TACGTA [CT]AC^GT[GA]
BsaBI 3476 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4908 GAGACC GGTCTC(1/5)
BsiEI 2426 CGGCCG CG[GA][CT]^CG
BsmI 3556 GAATGC GAATGC(1/-1)
BsoBI 3567 CTCGAG C^[CT]CG[GA]G
BspHI 2020 TCATGA T^CATGA
BspLU11I 2796 ACATGT A^CATGT
BspMI 574 GCAGGT ACCTGC(4/8)
Bst1107I 1033 GTATAC GTA^TAC
BstAPI 4392 GCATTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1738 GGTCACC G^GT[ACGT]ACC
BstPI 1738 GGTCACC G^GT[ACGT]ACC
BstUI 1279 CGCG CG^CG
BstZ17I 1033 GTATAC GTA^TAC
Bsu36I 1441 CCTGAGG CC^T[ACGT]AGG
CelII 4736 GCTCAGC GC^T[ACGT]AGC
DrdI 2472 GACTAAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2426 CGGCCG C^GGCCG
EclXI 2426 CGGCCG C^GGCCG
Eco47III 2931 AGCGCT AGC^GCT
Eco52I 2426 CGGCCG C^GGCCG
Eco81I 1441 CCTGAGG CC^T[ACGT]AGG
EcoNI 3647 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1738 GGTCACC G^GT[ACGT]ACC
FbaI 2017 TGATCA T^GATCA
HaeII 2931 AGCGCT [GA]GCGC^[CT]
HgaI 2479 GCGTC GACGC(5/10)
HindIII 1242 AAGCTT A^AGCTT
MamI 3476 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5554 CAATTG C^AATTG
MluNI 3189 TGGCCA TGG^CCA
MscI 3189 TGGCCA TGG^CCA
MunI 5554 CAATTG C^AATTG
MvnI 1279 CGCG CG^CG
NheI 5104 GCTAGC G^CTAGC
PaeR7I 3567 CTCGAG C^TCGAG
PshBI 1343 ATTAAT AT^TAAT
RcaI 2020 TCATGA T^CATGA
SbfI 5115 CCTGCAGG CCTGCA^GG
SnaBI 2754 TACGTA TAC^GTA
SpeI 2386 ACTAGT A^CTAGT
Sse8387I 5115 CCTGCAGG CCTGCA^GG
StuI 5273 AGGCCT AGG^CCT
SwaI 4626 ATTTAAAT ATTT^AAAT
XbaI 2578 TCTAGA T^CTAGA
XhoI 3567 CTCGAG C^TCGAG
XmnI 90 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 178 GACGTC GACGT^C
2883 GACGTC
AcyI 178 GACGTC G[GA]^CG[CT]C
2883 GACGTC
AhdI 5232 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5542 GACTTGTGGTC
AspEI 5232 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5542 GACTTGTGGTC
AvrII 5396 CCTAGG C^CTAGG
5468 CCTAGG
BanI 3917 GGTGCC G^G[CT][GA]CC
5506 GGTGCC
BbsI 4498 GAAGAC GAAGAC(2/6)
2353 GTCTTC
BcnI 2346 CCCGG CC^[GC]GG
2424 CCCGG
BglI 2413 GCCTTCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4555 GCCCTTCTGGC
BlnI 5396 CCTAGG C^CTAGG
5468 CCTAGG
BpuAI 4498 GAAGAC GAAGAC(2/6)
2353 GTCTTC
BsaHI 178 GACGTC G[GA]^CG[CT]C
2883 GACGTC
BsaWI 1431 ACCGGA [AT]^CCGG[AT]
1809 ACCGGA
BsmBI 5707 CGTCTC CGTCTC(1/5)
2881 GAGACG
BstXI 4456 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5510 CCAGAGGATTGG
CfoI 1280 GCGC GCG^C
2932 GCGC
EaeI 2426 CGGCCG [CT]^GGCC[GA]
3189 TGGCCA
Eam1105I 5232 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5542 GACTTGTGGTC
EcoRI 707 GAATTC G^AATTC
1153 GAATTC
FauI 4975 CCCGC CCCGC(4/6)
2179 GCGGG
HhaI 1280 GCGC GCG^C
2932 GCGC
Hin1I 178 GACGTC G[GA]^CG[CT]C
2883 GACGTC
HinP1I 1280 GCGC G^CGC
2932 GCGC
MslI 76 CATAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3623 CATTTGGATG
NciI 2346 CCCGG CC^[GC]GG
2424 CCCGG
NcoI 3922 CCATGG C^CATGG
4224 CCATGG
NdeI 296 CATATG CA^TATG
830 CATATG
PpuMI 4134 AGGTCCT [GA]G^G[AT]CC[CT]
5121 GGGACCC
SapI 1561 GCTCTTC GCTCTTC(1/4)
3033 GCTCTTC
SmlI 3567 CTCGAG C^T[CT][GA]AG
4059 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BamHI BanII BbvCI BbvI BcgI
BciVI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SphI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII