##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AflII AgeI AocI Aor51HI
AscI AseI AsnI AvrII BbeI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
Bsp1407I BspEI BsrBI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI HgaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI PacI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspBI SspI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1277 GACGTC GACGT^C
Acc65I 467 GGTACC G^GTACC
AclI 861 AACGTT AA^CGTT
AcyI 1277 GACGTC G[GA]^CG[CT]C
AhdI 4283 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 422 GGGCCC GGGCC^C
Asp700I 158 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 467 GGTACC G^GTACC
AspEI 4283 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1447 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 495 TGCGCA TGC^GCA
BbrPI 3835 CACGTG CAC^GTG
BclI 687 TGATCA T^GATCA
BglI 1205 GCCGTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1378 AGATCT A^GATCT
BsaHI 1277 GACGTC G[GA]^CG[CT]C
BsmBI 331 CGTCTC CGTCTC(1/5)
BspDI 6868 ATCGAT AT^CGAT
BsrFI 2385 ACCGGC [GA]^CCGG[CT]
BstBI 4436 TTCGAA TT^CGAA
Cfr10I 2385 ACCGGC [GA]^CCGG[CT]
ClaI 6868 ATCGAT AT^CGAT
Eam1105I 4283 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4018 GATATC GAT^ATC
FbaI 687 TGATCA T^GATCA
FspI 495 TGCGCA TGC^GCA
HaeII 1698 GGCGCT [GA]GCGC^[CT]
Hin1I 1277 GACGTC G[GA]^CG[CT]C
HindIII 6056 AAGCTT A^AGCTT
KpnI 467 GGTACC GGTAC^C
NdeI 4147 CATATG CA^TATG
NspV 4436 TTCGAA TT^CGAA
PaeR7I 5813 CTCGAG C^TCGAG
PflFI 1447 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3835 CACGTG CAC^GTG
PmlI 3835 CACGTG CAC^GTG
PshAI 2895 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 861 AACGTT AA^CGTT
SapI 629 GAAGAGC GCTCTTC(1/4)
SfuI 4436 TTCGAA TT^CGAA
SphI 3241 GCATGC GCATG^C
SwaI 6071 ATTTAAAT ATTT^AAAT
Tth111I 1447 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5241 TCTAGA T^CTAGA
XhoI 5813 CTCGAG C^TCGAG
XmnI 158 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 785 GTCTAC GT^[AC][GT]AC
4297 GTAGAC
AccII 374 CGCG CG^CG
2369 CGCG
Alw44I 1033 GTGCAC G^TGCAC
5945 GTGCAC
ApaLI 1033 GTGCAC G^TGCAC
5945 GTGCAC
BamHI 1023 GGATCC G^GATCC
5480 GGATCC
BbsI 805 GTCTTC GAAGAC(2/6)
2286 GTCTTC
BcgI 701 GCACGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6599 GCAAAGTTCTCG
BpuAI 805 GTCTTC GAAGAC(2/6)
2286 GTCTTC
BsaAI 788 TACGTG [CT]AC^GT[GA]
3835 CACGTG
BsaBI 2774 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4207 GATTGAAATC
BsaI 6250 GGTCTC GGTCTC(1/5)
6615 GGTCTC
BstAPI 2389 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2451 GCAAAATCTGC
BstUI 374 CGCG CG^CG
2369 CGCG
DraIII 1740 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
4094 CACCCTGTG
EcoT22I 1601 ATGCAT ATGCA^T
4073 ATGCAT
FauI 1900 CCCGC CCCGC(4/6)
2367 CCCGC
HpaI 3137 GTTAAC GTT^AAC
5380 GTTAAC
MamI 2774 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4207 GATTGAAATC
MvnI 374 CGCG CG^CG
2369 CGCG
NsiI 1601 ATGCAT ATGCA^T
4073 ATGCAT
ScaI 4482 AGTACT AGT^ACT
5817 AGTACT
SpeI 3521 ACTAGT A^CTAGT
4966 ACTAGT
XcmI 1258 CCAGAGTGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4960 CCAGTCACTAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII