##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI ApaI AscI AviII BbeI
BbrPI BcgI BfrI BglII BsaHI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BspMI BsrBI BssHII ClaI
CpoI EagI EclXI Eco52I EcoRV
FseI FspI Hin1I KasI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2323 GTATAC GT^[AC][GT]AC
AflIII 366 ACACGT A^C[GA][CT]GT
Alw44I 2303 GTGCAC G^TGCAC
ApaLI 2303 GTGCAC G^TGCAC
AseI 3234 ATTAAT AT^TAAT
AsnI 3234 ATTAAT AT^TAAT
AspI 4641 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3164 CCTCAGC CCTCAGC(-5/-2)
BclI 518 TGATCA T^GATCA
BglI 2527 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2771 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2771 GCTCAGC GC^T[ACGT]AGC
BsaAI 3357 TACGTG [CT]AC^GT[GA]
BsaBI 609 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 74 GGTCTC GGTCTC(1/5)
BsaWI 998 ACCGGA [AT]^CCGG[AT]
BspHI 206 TCATGA T^CATGA
BsrFI 236 ACCGGC [GA]^CCGG[CT]
BssSI 99 CTCGTG CACGAG(-5/-1)
Bst1107I 2323 GTATAC GTA^TAC
BstBI 1703 TTCGAA TT^CGAA
BstZ17I 2323 GTATAC GTA^TAC
CelII 2771 GCTCAGC GC^T[ACGT]AGC
Cfr10I 236 ACCGGC [GA]^CCGG[CT]
DraIII 2206 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1587 GAATTC G^AATTC
EcoT22I 1036 ATGCAT ATGCA^T
FbaI 518 TGATCA T^GATCA
HpaI 3042 GTTAAC GTT^AAC
KspI 52 CCGCGG CCGC^GG
MamI 609 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4356 CCATGG C^CATGG
NdeI 2920 CATATG CA^TATG
NsiI 1036 ATGCAT ATGCA^T
NspV 1703 TTCGAA TT^CGAA
PflFI 4641 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3234 ATTAAT AT^TAAT
RcaI 206 TCATGA T^CATGA
SacII 52 CCGCGG CCGC^GG
SfuI 1703 TTCGAA TT^CGAA
SphI 1956 GCATGC GCATG^C
StuI 2274 AGGCCT AGG^CCT
Tth111I 4641 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 61 GGTACC G^GTACC
3470 GGTACC
AhdI 856 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4890 GACCTGTTGTC
AocI 4258 CCTAAGG CC^T[ACGT]AGG
4813 CCTGAGG
Aor51HI 230 AGCGCT AGC^GCT
3228 AGCGCT
Asp718I 61 GGTACC G^GTACC
3470 GGTACC
AspEI 856 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4890 GACCTGTTGTC
AvrII 2700 CCTAGG C^CTAGG
4636 CCTAGG
BanII 1277 GAGCCC G[GA]GC[CT]^C
4216 GAGCCC
BciVI 4797 GGATAC GTATCC(6/5)
2884 GTATCC
BlnI 2700 CCTAGG C^CTAGG
4636 CCTAGG
BsmBI 3128 GAGACG CGTCTC(1/5)
3709 GAGACG
BsmI 2654 GAATGC GAATGC(1/-1)
2330 GCATTC
Bsp1407I 3223 TGTACA T^GTACA
4097 TGTACA
BsrGI 3223 TGTACA T^GTACA
4097 TGTACA
BstAPI 1057 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2205 GCACAGAGTGC
BstEII 3156 GGTCACC G^GT[ACGT]ACC
4340 GGTCACC
BstPI 3156 GGTCACC G^GT[ACGT]ACC
4340 GGTCACC
Bsu36I 4258 CCTAAGG CC^T[ACGT]AGG
4813 CCTGAGG
DraI 268 TTTAAA TTT^AAA
3549 TTTAAA
DsaI 52 CCGCGG C^C[GA][CT]GG
4356 CCATGG
Eam1105I 856 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4890 GACCTGTTGTC
Eco47III 230 AGCGCT AGC^GCT
3228 AGCGCT
Eco81I 4258 CCTAAGG CC^T[ACGT]AGG
4813 CCTGAGG
EcoO65I 3156 GGTCACC G^GT[ACGT]ACC
4340 GGTCACC
HincII 2782 GTCAAC GT[CT]^[GA]AC
3042 GTTAAC
HindII 2782 GTCAAC GT[CT]^[GA]AC
3042 GTTAAC
HindIII 3544 AAGCTT A^AGCTT
3769 AAGCTT
KpnI 61 GGTACC GGTAC^C
3470 GGTACC
NheI 2523 GCTAGC G^CTAGC
4613 GCTAGC
PvuII 184 CAGCTG CAG^CTG
1144 CAGCTG
SmaI 107 CCCGGG CCC^GGG
1931 CCCGGG
SspBI 3223 TGTACA T^GTACA
4097 TGTACA
XbaI 3983 TCTAGA T^CTAGA
4759 TCTAGA
XcmI 1352 CCATGACTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3866 CCACTGTCAGCCTGG
XmaCI 107 CCCGGG C^CCGGG
1931 CCCGGG
XmaI 107 CCCGGG C^CCGGG
1931 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI