##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BciVI
BclI BfrI BlnI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I CpoI DraI DrdI
Eam1105I Eco47III EcoRI EcoRV FbaI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MseI
MunI NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SfuI SnaBI SpeI
SspBI SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 1555 TCCGGA T^CCGGA
AgeI 3050 ACCGGT A^CCGGT
BseAI 1555 TCCGGA T^CCGGA
BspDI 984 ATCGAT AT^CGAT
BspEI 1555 TCCGGA T^CCGGA
BstAPI 20 GCACGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1068 GGTGACC G^GT[ACGT]ACC
BstPI 1068 GGTGACC G^GT[ACGT]ACC
ClaI 984 ATCGAT AT^CGAT
EcoO65I 1068 GGTGACC G^GT[ACGT]ACC
EcoT22I 3704 ATGCAT ATGCA^T
FseI 301 GGCCGGCC GGCCGG^CC
MroI 1555 TCCGGA T^CCGGA
NdeI 4235 CATATG CA^TATG
NsiI 3704 ATGCAT ATGCA^T
PinAI 3050 ACCGGT A^CCGGT
PleI 1041 GACTC GAGTC(4/5)
SbfI 1301 CCTGCAGG CCTGCA^GG
SexAI 2328 ACCAGGT A^CC[AT]GGT
SfiI 4277 GGCCCTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3049 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 1301 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BbeI 341 GGCGCC GGCGC^C
1338 GGCGCC
BcgI 463 CGATGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4694 GCAGGAGAATCG
BsaWI 1555 TCCGGA [AT]^CCGG[AT]
3050 ACCGGT
DraIII 226 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3020 CACGCTGTG
KasI 341 GGCGCC G^GCGCC
1338 GGCGCC
NaeI 302 GCCGGC GCC^GGC
4283 GCCGGC
NarI 341 GGCGCC GG^CGCC
1338 GGCGCC
NgoMIV 302 GCCGGC G^CCGGC
4283 GCCGGC
SacI 972 GAGCTC GAGCT^C
1128 GAGCTC
SfoI 341 GGCGCC GGC^GCC
1338 GGCGCC
SphI 962 GCATGC GCATG^C
1367 GCATGC
TaqI 850 TCGA T^CGA
985 TCGA
TthHB8I 850 TCGA T^CGA
985 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PmaCI PmlI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI TseI Tsp45I TspRI Van91I
XcmI XhoII XmaCI XmaI