##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        Aor51HI     AscI        Asp718I     AspI        
AviII       BcgI        BciVI       BclI        BglII       
BseAI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BssHII      Bst1107I    BstAPI      BstZ17I     
ClaI        CpoI        DraI        DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KpnI        MfeI        MluI        MroI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PflFI       PinAI       PmeI        
PshAI       Psp1406I    RsrII       SalI        SapI        
SbfI        SfiI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflII        3674  CTTAAG               C^TTAAG
AhdI         1992  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        510  GTGCAC               G^TGCAC
ApaLI         510  GTGCAC               G^TGCAC
AseI         3476  ATTAAT               AT^TAAT
AsnI         3476  ATTAAT               AT^TAAT
AspEI        1992  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        3013  CCTAGG               C^CTAGG
BamHI        1950  GGATCC               G^GATCC
BbrPI         168  CACGTG               CAC^GTG
BfrI         3674  CTTAAG               C^TTAAG
BlnI         3013  CCTAGG               C^CTAGG
BsaAI         168  CACGTG               [CT]AC^GT[GA]
BsaBI         932  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2880  GGTCTC               GGTCTC(1/5)
BsaWI        3397  ACCGGA               [AT]^CCGG[AT]
BsiEI          38  CGATCG               CG[GA][CT]^CG
BspLU11I      838  ACATGT               A^CATGT
BsrDI        3760  CATTGC               GCAATG(2/0)
BsrFI        1732  ACCGGC               [GA]^CCGG[CT]
BstBI        2219  TTCGAA               TT^CGAA
BstEII       1883  GGTTACC              G^GT[ACGT]ACC
BstPI        1883  GGTTACC              G^GT[ACGT]ACC
Cfr10I       1732  ACCGGC               [GA]^CCGG[CT]
DraIII        115  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1992  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1883  GGTTACC              G^GT[ACGT]ACC
EcoRI        3788  GAATTC               G^AATTC
KspI         1387  CCGCGG               CCGC^GG
MamI          932  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI          664  CATATG               CA^TATG
NspV         2219  TTCGAA               TT^CGAA
PflMI        3072  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         168  CACGTG               CAC^GTG
PmlI          168  CACGTG               CAC^GTG
PshBI        3476  ATTAAT               AT^TAAT
PvuI           38  CGATCG               CGAT^CG
SacII        1387  CCGCGG               CCGC^GG
ScaI          806  AGTACT               AGT^ACT
SfuI         2219  TTCGAA               TT^CGAA
SspI          531  AATATT               AAT^ATT
StuI         2686  AGGCCT               AGG^CCT
Van91I       3072  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII        643  ACACGT               A^C[GA][CT]GT
              838  ACATGT              
Asp700I       308  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1192  GAACAAGTTC          
BalI         1718  TGGCCA               TGG^CCA
             3989  TGGCCA              
BbvCI        4379  CCTCAGC              CCTCAGC(-5/-2)
              188  GCTGAGG             
BspMI        2957  ACCTGC               ACCTGC(4/8)
             3038  ACCTGC              
BssSI        1487  CACGAG               CACGAG(-5/-1)
              444  CTCGTG              
HgaI          392  GACGC                GACGC(5/10)
             4555  GCGTC               
HincII        842  GTCAAC               GT[CT]^[GA]AC
             4689  GTTGAC              
HindII        842  GTCAAC               GT[CT]^[GA]AC
             4689  GTTGAC              
MluNI        1718  TGGCCA               TGG^CCA
             3989  TGGCCA              
MscI         1718  TGGCCA               TGG^CCA
             3989  TGGCCA              
MslI         2183  CACGGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4835  CACTGGCGTG          
NspI          838  ACATGT               [GA]CATG^[CT]
             4507  GCATGT              
PvuII         285  CAGCTG               CAG^CTG
             2237  CAGCTG              
SacI         2336  GAGCTC               GAGCT^C
             4292  GAGCTC              
SexAI         400  ACCTGGT              A^CC[AT]GGT
             4785  ACCTGGT             
SmaI           29  CCCGGG               CCC^GGG
             1556  CCCGGG              
XbaI         3467  TCTAGA               T^CTAGA
             3982  TCTAGA              
XmaCI          29  CCCGGG               C^CCGGG
             1556  CCCGGG              
XmaI           29  CCCGGG               C^CCGGG
             1556  CCCGGG              
XmnI          308  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1192  GAACAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbeI        BbsI        BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspHI       
BsrBI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PaeR7I      
PleI        PpuMI       PstI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoI        XhoII