##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvrII       BbeI        BbrPI       
BcgI        BciVI       BfrI        BglII       BlnI        
BsaAI       BsiWI       BspDI       BspHI       BspLU11I    
BspMI       BsrDI       Bst1107I    BstBI       BstZ17I     
ClaI        DraI        DrdI        EcoRV       EcoT22I     
HincII      HindII      HpaI        KasI        KpnI        
MfeI        MluI        MunI        NarI        NcoI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         3349  GTCTAC               GT^[AC][GT]AC
AccIII       3898  TCCGGA               T^CCGGA
AflIII       3088  ACGTGT               A^C[GA][CT]GT
AhdI         4653  GACTCTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        4653  GACTCTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         724  TGCGCA               TGC^GCA
BamHI        1922  GGATCC               G^GATCC
BbsI         4321  GTCTTC               GAAGAC(2/6)
BbvCI        3052  CCTCAGC              CCTCAGC(-5/-2)
BpuAI        4321  GTCTTC               GAAGAC(2/6)
BseAI        3898  TCCGGA               T^CCGGA
BsmI         3608  GAATGC               GAATGC(1/-1)
Bsp1407I     4047  TGTACA               T^GTACA
BspEI        3898  TCCGGA               T^CCGGA
BsrGI        4047  TGTACA               T^GTACA
BstEII       3930  GGTGACC              G^GT[ACGT]ACC
BstPI        3930  GGTGACC              G^GT[ACGT]ACC
CpoI         3895  CGGTCCG              CG^G[AT]CCG
EagI          225  CGGCCG               C^GGCCG
Eam1105I     4653  GACTCTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         225  CGGCCG               C^GGCCG
Eco52I        225  CGGCCG               C^GGCCG
EcoO65I      3930  GGTGACC              G^GT[ACGT]ACC
EcoRI        3639  GAATTC               G^AATTC
FseI         2452  GGCCGGCC             GGCCGG^CC
FspI          724  TGCGCA               TGC^GCA
HindIII      1743  AAGCTT               A^AGCTT
MroI         3898  TCCGGA               T^CCGGA
MseI         3292  TTAA                 T^TAA
NdeI         3175  CATATG               CA^TATG
PaeR7I        882  CTCGAG               C^TCGAG
PshAI        4216  GACGGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        3895  CGGTCCG              CG^G[AT]CCG
SspBI        4047  TGTACA               T^GTACA
Tru9I        3292  TTAA                 T^TAA
XhoI          882  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI         3531  GTAC                 GT^AC
             4048  GTAC                
AocI         2418  CCTAAGG              CC^T[ACGT]AGG
             3658  CCTTAGG             
Aor51HI      1261  AGCGCT               AGC^GCT
             1660  AGCGCT              
BclI         4197  TGATCA               T^GATCA
             4691  TGATCA              
BlpI          551  GCTCAGC              GC^T[ACGT]AGC
             1643  GCTGAGC             
Bpu1102I      551  GCTCAGC              GC^T[ACGT]AGC
             1643  GCTGAGC             
BsaBI        1805  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2978  GATCGGCATC          
BstXI         997  CCAATGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3860  CCAGACTTCTGG        
Bsu36I       2418  CCTAAGG              CC^T[ACGT]AGG
             3658  CCTTAGG             
CelII         551  GCTCAGC              GC^T[ACGT]AGC
             1643  GCTGAGC             
DraIII       3339  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4684  CACTCAGTG           
Eco47III     1261  AGCGCT               AGC^GCT
             1660  AGCGCT              
Eco81I       2418  CCTAAGG              CC^T[ACGT]AGG
             3658  CCTTAGG             
FbaI         4197  TGATCA               T^GATCA
             4691  TGATCA              
KspI          303  CCGCGG               CCGC^GG
             4232  CCGCGG              
MamI         1805  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2978  GATCGGCATC          
MslI          728  CATGACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2855  CACCGTGGTG          
NaeI          370  GCCGGC               GCC^GGC
             2453  GCCGGC              
NgoMIV        370  GCCGGC               G^CCGGC
             2453  GCCGGC              
NheI         1332  GCTAGC               G^CTAGC
             2744  GCTAGC              
NspI         2887  GCATGC               [GA]CATG^[CT]
             4266  GCATGC              
RsaI         3531  GTAC                 GT^AC
             4048  GTAC                
SacII         303  CCGCGG               CCGC^GG
             4232  CCGCGG              
SphI         2887  GCATGC               GCATG^C
             4266  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AluI        
AlwI        AlwNI       ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrFI       BsrI        
BssHII      BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        XmnI