##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      ApaLI       AscI        AspEI       AspHI       
BbeI        BbrPI       BbvCI       BcgI        BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspEI       BsrGI       BssHII      BstBI       BstEII      
BstPI       CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRV       FseI        
KasI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacI        SexAI       SfiI        SfoI        
SfuI        SmaI        SpeI        SphI        SspBI       
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflII        4358  CTTAAG               C^TTAAG
AocI         5001  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      5404  AGCGCT               AGC^GCT
ApaI          149  GGGCCC               GGGCC^C
Asp700I      5431  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         3397  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1695  CTCGGG               C^[CT]CG[GA]G
AviII        5121  TGCGCA               TGC^GCA
BciVI        4383  GTATCC               GTATCC(6/5)
BclI         2833  TGATCA               T^GATCA
BfrI         4358  CTTAAG               C^TTAAG
BglII         213  AGATCT               A^GATCT
BlpI          194  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      194  GCTGAGC              GC^T[ACGT]AGC
BsaAI        5086  TACGTA               [CT]AC^GT[GA]
BsaBI        1928  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2420  GGTCTC               GGTCTC(1/5)
BsaWI         574  TCCGGT               [AT]^CCGG[AT]
BsoBI        1695  CTCGGG               C^[CT]CG[GA]G
BspHI        4789  TCATGA               T^CATGA
BsrBI          43  GAGCGG               CCGCTC(-3/-3)
BsrFI        2871  ACCGGC               [GA]^CCGG[CT]
BssSI        2034  CTCGTG               CACGAG(-5/-1)
Bst1107I     2312  GTATAC               GTA^TAC
BstZ17I      2312  GTATAC               GTA^TAC
Bsu36I       5001  CCTAAGG              CC^T[ACGT]AGG
CelII         194  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       2871  ACCGGC               [GA]^CCGG[CT]
Eco47III     5404  AGCGCT               AGC^GCT
Eco81I       5001  CCTAAGG              CC^T[ACGT]AGG
EcoNI        3168  CCTCCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         2833  TGATCA               T^GATCA
FspI         5121  TGCGCA               TGC^GCA
HpaI         2101  GTTAAC               GTT^AAC
KspI          153  CCGCGG               CCGC^GG
MamI         1928  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI        3397  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1693  GACTCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         629  CAGCTG               CAG^CTG
RcaI         4789  TCATGA               T^CATGA
SacII         153  CCGCGG               CCGC^GG
SalI          548  GTCGAC               G^TCGAC
SbfI         2617  CCTGCAGG             CCTGCA^GG
SgrAI        2870  CACCGGCG             C[GA]^CCGG[CT]G
SnaBI        5086  TACGTA               TAC^GTA
Sse8387I     2617  CCTGCAGG             CCTGCA^GG
Tth111I      3397  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         5186  TCTAGA               T^CTAGA
XmnI         5431  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          588  GACGCC               G[GA]^CG[CT]C
             3397  GACGCC              
AseI         5465  ATTAAT               AT^TAAT
             5546  ATTAAT              
AsnI         5465  ATTAAT               AT^TAAT
             5546  ATTAAT              
AvrII        3377  CCTAGG               C^CTAGG
             4919  CCTAGG              
BamHI        2066  GGATCC               G^GATCC
             2992  GGATCC              
BanII         149  GGGCCC               G[GA]GC[CT]^C
             1676  GAGCCC              
BglI          591  GCCCTTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1007  GCCACAGTGGC         
BlnI         3377  CCTAGG               C^CTAGG
             4919  CCTAGG              
BsaHI         588  GACGCC               G[GA]^CG[CT]C
             3397  GACGCC              
BseRI        4530  CTCCTC               GAGGAG(10/8)
             4542  CTCCTC              
BsgI         5154  CTGCAC               GTGCAG(16/14)
             1879  GTGCAG              
BspDI        1598  ATCGAT               AT^CGAT
             3004  ATCGAT              
ClaI         1598  ATCGAT               AT^CGAT
             3004  ATCGAT              
DraI         5355  TTTAAA               TTT^AAA
             5704  TTTAAA              
DraII        1254  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             3015  AGGTCCC             
DrdI         1901  GACAAAAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2429  GACAGCCCTGTC        
EcoO109I     1254  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             3015  AGGTCCC             
EcoRI        2162  GAATTC               G^AATTC
             5529  GAATTC              
EcoT22I       867  ATGCAT               ATGCA^T
             3882  ATGCAT              
HaeII         135  AGCGCC               [GA]GCGC^[CT]
             5404  AGCGCT              
Hin1I         588  GACGCC               G[GA]^CG[CT]C
             3397  GACGCC              
HindIII      1581  AAGCTT               A^AGCTT
             2819  AAGCTT              
NsiI          867  ATGCAT               ATGCA^T
             3882  ATGCAT              
PflMI        1544  CCAAGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2510  CCACCCAATGG         
PpuMI        1254  GGGTCCT              [GA]G^G[AT]CC[CT]
             3015  AGGTCCC             
PshBI        5465  ATTAAT               AT^TAAT
             5546  ATTAAT              
SapI         2629  GAAGAGC              GCTCTTC(1/4)
             2573  GCTCTTC             
StuI         1547  AGGCCT               AGG^CCT
             3486  AGGCCT              
Van91I       1544  CCAAGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2510  CCACCCAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcsI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        Asp718I     AvaII       BalI        BanI        
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspLU11I    
BspMI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII