##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI Aor51HI ApaI AscI AvrII
BamHI BbeI BbrPI BciVI BfrI
BlnI BsaAI BsiEI BsiWI BsmBI
BspDI BspLU11I BsrBI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI HaeII HpaI KasI
KspI MluI MvnI NarI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 4609 TCCGGA T^CCGGA
AcyI 2892 GGCGTC G[GA]^CG[CT]C
AhdI 4686 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3189 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 4686 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4794 TGCGCA TGC^GCA
BbvCI 3761 GCTGAGG CCTCAGC(-5/-2)
BcgI 2201 GCAAAGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 940 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4202 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4202 GCTAAGC GC^T[ACGT]AGC
BsaBI 1827 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2892 GGCGTC G[GA]^CG[CT]C
BsaI 4736 GAGACC GGTCTC(1/5)
BsaWI 4609 TCCGGA [AT]^CCGG[AT]
BseAI 4609 TCCGGA T^CCGGA
BspEI 4609 TCCGGA T^CCGGA
BsrFI 2115 GCCGGC [GA]^CCGG[CT]
BssSI 1261 CACGAG CACGAG(-5/-1)
CelII 4202 GCTAAGC GC^T[ACGT]AGC
CfoI 4795 GCGC GCG^C
Cfr10I 2115 GCCGGC [GA]^CCGG[CT]
Eam1105I 4686 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3767 GAATTC G^AATTC
EcoT22I 587 ATGCAT ATGCA^T
FspI 4794 TGCGCA TGC^GCA
HhaI 4795 GCGC GCG^C
Hin1I 2892 GGCGTC G[GA]^CG[CT]C
HinP1I 4795 GCGC G^CGC
HindIII 154 AAGCTT A^AGCTT
MamI 1827 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3676 CAATTG C^AATTG
MroI 4609 TCCGGA T^CCGGA
MunI 3676 CAATTG C^AATTG
NaeI 2115 GCCGGC GCC^GGC
NgoMIV 2115 GCCGGC G^CCGGC
NsiI 587 ATGCAT ATGCA^T
SacI 1066 GAGCTC GAGCT^C
SapI 1323 GCTCTTC GCTCTTC(1/4)
SmaI 2450 CCCGGG CCC^GGG
SmlI 2432 CTTGAG C^T[CT][GA]AG
SphI 1474 GCATGC GCATG^C
StuI 947 AGGCCT AGG^CCT
XcmI 4672 CCATGAGTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2450 CCCGGG C^CCGGG
XmaI 2450 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1454 GGTACC G^GTACC
3253 GGTACC
AccI 46 GTAGAC GT^[AC][GT]AC
879 GTCTAC
AocI 55 CCTTAGG CC^T[ACGT]AGG
4379 CCTAAGG
AseI 1416 ATTAAT AT^TAAT
1862 ATTAAT
AsnI 1416 ATTAAT AT^TAAT
1862 ATTAAT
Asp700I 785 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1930 GAAGTAGTTC
Asp718I 1454 GGTACC G^GTACC
3253 GGTACC
BalI 616 TGGCCA TGG^CCA
2966 TGGCCA
BglII 1981 AGATCT A^GATCT
4762 AGATCT
BsgI 195 CTGCAC GTGCAG(16/14)
1903 GTGCAG
BstEII 1762 GGTCACC G^GT[ACGT]ACC
3681 GGTGACC
BstPI 1762 GGTCACC G^GT[ACGT]ACC
3681 GGTGACC
BstXI 977 CCAGATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4292 CCATTACAATGG
Bsu36I 55 CCTTAGG CC^T[ACGT]AGG
4379 CCTAAGG
DraI 1973 TTTAAA TTT^AAA
2147 TTTAAA
EaeI 616 TGGCCA [CT]^GGCC[GA]
2966 TGGCCA
EarI 1324 CTCTTC CTCTTC(1/4)
4400 CTCTTC
Eco81I 55 CCTTAGG CC^T[ACGT]AGG
4379 CCTAAGG
EcoNI 2547 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3120 CCTTCAGCAGG
EcoO65I 1762 GGTCACC G^GT[ACGT]ACC
3681 GGTGACC
FauI 2659 GCGGG CCCGC(4/6)
2841 GCGGG
KpnI 1454 GGTACC GGTAC^C
3253 GGTACC
Ksp632I 1324 CTCTTC CTCTTC(1/4)
4400 CTCTTC
MluNI 616 TGGCCA TGG^CCA
2966 TGGCCA
MscI 616 TGGCCA TGG^CCA
2966 TGGCCA
MspA1I 2496 CAGCTG C[AC]G^C[GT]G
2911 CAGCTG
NheI 2051 GCTAGC G^CTAGC
2655 GCTAGC
PflMI 927 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3483 CCATCTCTTGG
PpuMI 1721 AGGACCC [GA]G^G[AT]CC[CT]
1782 GGGTCCC
PshBI 1416 ATTAAT AT^TAAT
1862 ATTAAT
PvuII 2496 CAGCTG CAG^CTG
2911 CAGCTG
SexAI 1529 ACCTGGT A^CC[AT]GGT
4222 ACCTGGT
Van91I 927 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3483 CCATCTCTTGG
XbaI 3797 TCTAGA T^CTAGA
4067 TCTAGA
XmnI 785 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1930 GAAGTAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DraIII DsaI EcoO109I
EcoRII EcoRV EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII