##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI BbeI BsaWI BseAI BsiWI
BspDI BspEI BssHII BstBI ClaI
CpoI DrdI EcoT22I FseI HindIII
KasI KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1317 GGTACC G^GTACC
AccII 158 CGCG CG^CG
AcyI 3719 GACGCC G[GA]^CG[CT]C
AhdI 551 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 383 GTGCAC G^TGCAC
Aor51HI 3789 AGCGCT AGC^GCT
ApaLI 383 GTGCAC G^TGCAC
AseI 4676 ATTAAT AT^TAAT
AsnI 4676 ATTAAT AT^TAAT
Asp718I 1317 GGTACC G^GTACC
AspEI 551 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 142 CCTAGG C^CTAGG
BcgI 1048 CGACAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 142 CCTAGG C^CTAGG
BsaBI 2143 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3719 GACGCC G[GA]^CG[CT]C
BsiEI 1234 CGGCCG CG[GA][CT]^CG
BspHI 769 TCATGA T^CATGA
BssSI 1604 CTCGTG CACGAG(-5/-1)
Bst1107I 3484 GTATAC GTA^TAC
BstUI 158 CGCG CG^CG
BstZ17I 3484 GTATAC GTA^TAC
EagI 1234 CGGCCG C^GGCCG
Eam1105I 551 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1234 CGGCCG C^GGCCG
Eco47III 3789 AGCGCT AGC^GCT
Eco52I 1234 CGGCCG C^GGCCG
EcoRV 1580 GATATC GAT^ATC
Hin1I 3719 GACGCC G[GA]^CG[CT]C
KpnI 1317 GGTACC GGTAC^C
MamI 2143 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 158 CGCG CG^CG
NaeI 5195 GCCGGC GCC^GGC
NcoI 1761 CCATGG C^CATGG
NgoMIV 5195 GCCGGC G^CCGGC
PaeR7I 1376 CTCGAG C^TCGAG
PshBI 4676 ATTAAT AT^TAAT
RcaI 769 TCATGA T^CATGA
SapI 856 GCTCTTC GCTCTTC(1/4)
XbaI 2413 TCTAGA T^CTAGA
XhoI 1376 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspI 356 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1265 GACGTTGTC
AviII 264 TGCGCA TGC^GCA
2277 TGCGCA
BciVI 1140 GTATCC GTATCC(6/5)
5075 GTATCC
BclI 3511 TGATCA T^GATCA
4036 TGATCA
BglII 4535 AGATCT A^GATCT
5375 AGATCT
BsaI 2249 GAGACC GGTCTC(1/5)
3176 GAGACC
BsmBI 2034 GAGACG CGTCTC(1/5)
2870 GAGACG
BspLU11I 2045 ACATGT A^CATGT
4162 ACATGT
BsrFI 2003 ACCGGC [GA]^CCGG[CT]
5195 GCCGGC
BstEII 181 GGTGACC G^GT[ACGT]ACC
2498 GGTGACC
BstPI 181 GGTGACC G^GT[ACGT]ACC
2498 GGTGACC
Cfr10I 2003 ACCGGC [GA]^CCGG[CT]
5195 GCCGGC
DraI 2323 TTTAAA TTT^AAA
5609 TTTAAA
DraIII 1163 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2600 CACATTGTG
EcoO65I 181 GGTGACC G^GT[ACGT]ACC
2498 GGTGACC
EcoRI 464 GAATTC G^AATTC
4089 GAATTC
FbaI 3511 TGATCA T^GATCA
4036 TGATCA
FspI 264 TGCGCA TGC^GCA
2277 TGCGCA
HpaI 1456 GTTAAC GTT^AAC
5101 GTTAAC
PflFI 356 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1265 GACGTTGTC
PflMI 2008 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2637 CCATCCTCTGG
PshAI 206 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
718 GACTCCTGTC
SacI 20 GAGCTC GAGCT^C
5451 GAGCTC
ScaI 4193 AGTACT AGT^ACT
4412 AGTACT
SphI 511 GCATGC GCATG^C
1768 GCATGC
SspI 795 AATATT AAT^ATT
5083 AATATT
Tth111I 356 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1265 GACGTTGTC
Van91I 2008 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2637 CCATCCTCTGG
XcmI 1924 CCATGCGCCGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4634 CCATCAGGGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BfrI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI