##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccIII AgeI AscI
Asp718I AvrII BamHI BbvCI BclI
BglI BlnI BsaBI BseAI BsiWI
BsmBI BspEI BspHI BssHII Bst1107I
BstBI BstZ17I CpoI EcoNI EcoRI
FbaI FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NotI NruI NspV
PinAI PmeI PvuI RcaI RsrII
SacII SalI SexAI SfiI SfuI
SgrAI SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AclI 5073 AACGTT AA^CGTT
AcyI 1458 GGCGCC G[GA]^CG[CT]C
AflII 3382 CTTAAG C^TTAAG
AhdI 3739 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1449 AGCGCT AGC^GCT
AseI 4148 ATTAAT AT^TAAT
AsnI 4148 ATTAAT AT^TAAT
AspEI 3739 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1409 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1299 TGCGCA TGC^GCA
BbeI 1458 GGCGCC GGCGC^C
BcgI 675 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3382 CTTAAG C^TTAAG
BsaHI 1458 GGCGCC G[GA]^CG[CT]C
BsaI 2201 GGTCTC GGTCTC(1/5)
BsaWI 669 ACCGGA [AT]^CCGG[AT]
BsmI 3405 GAATGC GAATGC(1/-1)
Bsp1407I 2978 TGTACA T^GTACA
BspDI 1940 ATCGAT AT^CGAT
BsrDI 3633 GCAATG GCAATG(2/0)
BsrGI 2978 TGTACA T^GTACA
BssSI 1289 CACGAG CACGAG(-5/-1)
BstEII 2042 GGTCACC G^GT[ACGT]ACC
BstPI 2042 GGTCACC G^GT[ACGT]ACC
ClaI 1940 ATCGAT AT^CGAT
DrdI 752 GACTTTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1088 CGGCCG C^GGCCG
Eam1105I 3739 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1088 CGGCCG C^GGCCG
Eco47III 1449 AGCGCT AGC^GCT
Eco52I 1088 CGGCCG C^GGCCG
EcoO65I 2042 GGTCACC G^GT[ACGT]ACC
EcoRV 5304 GATATC GAT^ATC
EcoT22I 3104 ATGCAT ATGCA^T
FspI 1299 TGCGCA TGC^GCA
Hin1I 1458 GGCGCC G[GA]^CG[CT]C
HincII 4115 GTTGAC GT[CT]^[GA]AC
HindII 4115 GTTGAC GT[CT]^[GA]AC
KasI 1458 GGCGCC G^GCGCC
NaeI 1455 GCCGGC GCC^GGC
NarI 1458 GGCGCC GG^CGCC
NcoI 4036 CCATGG C^CATGG
NgoMIV 1455 GCCGGC G^CCGGC
NheI 4857 GCTAGC G^CTAGC
NsiI 3104 ATGCAT ATGCA^T
PacI 2411 TTAATTAA TTAAT^TAA
PflFI 1409 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4370 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4148 ATTAAT AT^TAAT
Psp1406I 5073 AACGTT AA^CGTT
ScaI 3046 AGTACT AGT^ACT
SfoI 1458 GGCGCC GGC^GCC
SmaI 381 CCCGGG CCC^GGG
SspBI 2978 TGTACA T^GTACA
SspI 3489 AATATT AAT^ATT
StuI 924 AGGCCT AGG^CCT
Tth111I 1409 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 381 CCCGGG C^CCGGG
XmaI 381 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1262 GTGCAC G^TGCAC
2500 GTGCAC
AocI 3063 CCTCAGG CC^T[ACGT]AGG
5409 CCTTAGG
ApaLI 1262 GTGCAC G^TGCAC
2500 GTGCAC
BalI 237 TGGCCA TGG^CCA
3213 TGGCCA
BglII 894 AGATCT A^GATCT
1110 AGATCT
BlpI 1189 GCTCAGC GC^T[ACGT]AGC
2053 GCTGAGC
Bpu1102I 1189 GCTCAGC GC^T[ACGT]AGC
2053 GCTGAGC
BsiEI 1088 CGGCCG CG[GA][CT]^CG
1408 CGACCG
BsrBI 1099 CCGCTC CCGCTC(-3/-3)
1085 GAGCGG
BsrFI 774 ACCGGC [GA]^CCGG[CT]
1455 GCCGGC
BstAPI 862 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5285 GCAGACCCTGC
Bsu36I 3063 CCTCAGG CC^T[ACGT]AGG
5409 CCTTAGG
CelII 1189 GCTCAGC GC^T[ACGT]AGC
2053 GCTGAGC
Cfr10I 774 ACCGGC [GA]^CCGG[CT]
1455 GCCGGC
Eco81I 3063 CCTCAGG CC^T[ACGT]AGG
5409 CCTTAGG
HgaI 983 GACGC GACGC(5/10)
1413 GCGTC
MluNI 237 TGGCCA TGG^CCA
3213 TGGCCA
MscI 237 TGGCCA TGG^CCA
3213 TGGCCA
PaeR7I 965 CTCGAG C^TCGAG
5118 CTCGAG
SbfI 3275 CCTGCAGG CCTGCA^GG
4388 CCTGCAGG
SpeI 3655 ACTAGT A^CTAGT
4681 ACTAGT
SphI 3289 GCATGC GCATG^C
3937 GCATGC
Sse8387I 3275 CCTGCAGG CCTGCA^GG
4388 CCTGCAGG
XbaI 3187 TCTAGA T^CTAGA
4100 TCTAGA
XhoI 965 CTCGAG C^TCGAG
5118 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbrPI BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI