##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspI BbrPI BfrI BglII
BsiEI BsiWI BspDI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI TfiI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 2296 TCCGGA T^CCGGA
AcsI 4400 AAATTC [GA]^AATT[CT]
AhdI 501 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1718 CCTGAGG CC^T[ACGT]AGG
ApoI 4400 AAATTC [GA]^AATT[CT]
Asp700I 4396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 501 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3364 GAGCAC G[AT]GC[AT]^C
AvrII 3533 CCTAGG C^CTAGG
BbeI 2733 GGCGCC GGCGC^C
BciVI 2409 GGATAC GTATCC(6/5)
BclI 2935 TGATCA T^GATCA
BlnI 3533 CCTAGG C^CTAGG
BlpI 3415 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3415 GCTCAGC GC^T[ACGT]AGC
BsaAI 1068 TACGTG [CT]AC^GT[GA]
BsaWI 2296 TCCGGA [AT]^CCGG[AT]
BseAI 2296 TCCGGA T^CCGGA
BsiHKAI 3364 GAGCAC G[AT]GC[AT]^C
BsmI 2506 GCATTC GAATGC(1/-1)
Bsp1407I 2565 TGTACA T^GTACA
BspEI 2296 TCCGGA T^CCGGA
BspHI 3044 TCATGA T^CATGA
BspLU11I 3018 ACATGT A^CATGT
BsrDI 1671 GCAATG GCAATG(2/0)
BsrGI 2565 TGTACA T^GTACA
BssHII 2456 GCGCGC G^CGCGC
BssSI 306 CTCGTG CACGAG(-5/-1)
Bsu36I 1718 CCTGAGG CC^T[ACGT]AGG
CelII 3415 GCTCAGC GC^T[ACGT]AGC
DraIII 3937 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 501 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1238 GAAGAG CTCTTC(1/4)
Eco81I 1718 CCTGAGG CC^T[ACGT]AGG
FbaI 2935 TGATCA T^GATCA
KasI 2733 GGCGCC G^GCGCC
Ksp632I 1238 GAAGAG CTCTTC(1/4)
MroI 2296 TCCGGA T^CCGGA
MseI 3004 TTAA T^TAA
NarI 2733 GGCGCC GG^CGCC
NdeI 3593 CATATG CA^TATG
PaeR7I 1641 CTCGAG C^TCGAG
RcaI 3044 TCATGA T^CATGA
ScaI 973 AGTACT AGT^ACT
SexAI 1858 ACCTGGT A^CC[AT]GGT
SfoI 2733 GGCGCC GGC^GCC
SspBI 2565 TGTACA T^GTACA
SspI 3822 AATATT AAT^ATT
Tru9I 3004 TTAA T^TAA
XhoI 1641 CTCGAG C^TCGAG
XmnI 4396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Acc65I 2107 GGTACC G^GTACC
2383 GGTACC
Asp718I 2107 GGTACC G^GTACC
2383 GGTACC
BcgI 1907 CGACCCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2186 GCAGCGGCCTCG
BsaBI 1448 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1943 GATCCCCATC
HaeII 2733 GGCGCC [GA]GCGC^[CT]
3719 GGCGCT
HincII 837 GTCAAC GT[CT]^[GA]AC
1848 GTTGAC
HindII 837 GTCAAC GT[CT]^[GA]AC
1848 GTTGAC
KpnI 2107 GGTACC GGTAC^C
2383 GGTACC
KspI 4024 CCGCGG CCGC^GG
4096 CCGCGG
MamI 1448 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1943 GATCCCCATC
PstI 284 CTGCAG CTGCA^G
3271 CTGCAG
SacII 4024 CCGCGG CCGC^GG
4096 CCGCGG
SmaI 1346 CCCGGG CCC^GGG
3901 CCCGGG
SphI 1762 GCATGC GCATG^C
1786 GCATGC
XmaCI 1346 CCCGGG C^CCGGG
3901 CCCGGG
XmaI 1346 CCCGGG C^CCGGG
3901 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AvaI
AvaII AviII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII