##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BbrPI BcgI BciVI BfrI BglI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HgaI HpaI KpnI
MamI MluI MroI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 239 CGCG CG^CG
AcyI 3776 GGCGCC G[GA]^CG[CT]C
AhdI 4384 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1177 AGCGCT AGC^GCT
AspEI 4384 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1940 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 274 GGATCC G^GATCC
BbeI 3776 GGCGCC GGCGC^C
BclI 1298 TGATCA T^GATCA
BglII 3452 AGATCT A^GATCT
BlpI 396 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 396 GCTAAGC GC^T[ACGT]AGC
BsaAI 2665 TACGTG [CT]AC^GT[GA]
BsaHI 3776 GGCGCC G[GA]^CG[CT]C
BsmI 866 GAATGC GAATGC(1/-1)
BspMI 2887 ACCTGC ACCTGC(4/8)
BsrBI 3059 GAGCGG CCGCTC(-3/-3)
BssSI 3554 CTCGTG CACGAG(-5/-1)
BstUI 239 CGCG CG^CG
CelII 396 GCTAAGC GC^T[ACGT]AGC
DrdI 468 GACAGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4384 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1177 AGCGCT AGC^GCT
FbaI 1298 TGATCA T^GATCA
Hin1I 3776 GGCGCC G[GA]^CG[CT]C
KasI 3776 GGCGCC G^GCGCC
KspI 238 CCGCGG CCGC^GG
MfeI 3867 CAATTG C^AATTG
MunI 3867 CAATTG C^AATTG
MvnI 239 CGCG CG^CG
NarI 3776 GGCGCC GG^CGCC
NdeI 3224 CATATG CA^TATG
PaeR7I 3885 CTCGAG C^TCGAG
PflFI 1940 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 238 CCGCGG CCGC^GG
SapI 3802 GAAGAGC GCTCTTC(1/4)
ScaI 4803 AGTACT AGT^ACT
SfoI 3776 GGCGCC GGC^GCC
Tth111I 1940 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 893 CCAGAGGAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3885 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Asp700I 1542 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4211 GAAACCCTTC
BstAPI 46 GCAATCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4637 GCAGCGGTTGC
BstYI 274 GGATCC [GA]^GATC[CT]
3452 AGATCT
EcoRI 560 GAATTC G^AATTC
4688 GAATTC
HincII 1011 GTTGAC GT[CT]^[GA]AC
1616 GTTGAC
HindII 1011 GTTGAC GT[CT]^[GA]AC
1616 GTTGAC
HindIII 176 AAGCTT A^AGCTT
3122 AAGCTT
MflI 274 GGATCC [GA]^GATC[CT]
3452 AGATCT
NaeI 3782 GCCGGC GCC^GGC
3910 GCCGGC
NgoMIV 3782 GCCGGC G^CCGGC
3910 GCCGGC
PflMI 813 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4387 CCAGGGTCTGG
PpuMI 1002 AGGACCT [GA]G^G[AT]CC[CT]
2806 GGGTCCT
StuI 770 AGGCCT AGG^CCT
2746 AGGCCT
Van91I 813 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4387 CCAGGGTCTGG
XhoII 274 GGATCC [GA]^GATC[CT]
3452 AGATCT
XmnI 1542 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4211 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI