##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AclI AhdI Aor51HI AscI
Asp718I AspEI AviII BbrPI BglI
BsaAI BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII BstAPI BstBI ClaI
CpoI Eam1105I Eco47III EcoT22I FseI
FspI HpaI KpnI KspI MluI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SfiI SfuI SgrAI SnaBI SpeI
SspBI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 4923 GACGTC GACGT^C
AccIII 137 TCCGGA T^CCGGA
AgeI 2007 ACCGGT A^CCGGT
Alw44I 5491 GTGCAC G^TGCAC
ApaLI 5491 GTGCAC G^TGCAC
Asp700I 5562 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 197 CCTAGG C^CTAGG
BcgI 1654 CGAGTATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 393 GGATAC GTATCC(6/5)
BlnI 197 CCTAGG C^CTAGG
BsaBI 2240 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 137 TCCGGA T^CCGGA
BsmBI 1006 GAGACG CGTCTC(1/5)
BspEI 137 TCCGGA T^CCGGA
BspLU11I 3950 ACATGT A^CATGT
BssSI 861 CTCGTG CACGAG(-5/-1)
Bst1107I 5296 GTATAC GTA^TAC
BstZ17I 5296 GTATAC GTA^TAC
DrdI 3590 GACTTTATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 16 CGGCCG C^GGCCG
EclXI 16 CGGCCG C^GGCCG
Eco52I 16 CGGCCG C^GGCCG
EcoRI 3332 GAATTC G^AATTC
EcoRV 1748 GATATC GAT^ATC
HgaI 1008 GACGC GACGC(5/10)
HincII 4926 GTCAAC GT[CT]^[GA]AC
HindII 4926 GTCAAC GT[CT]^[GA]AC
MamI 2240 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5818 CAATTG C^AATTG
MroI 137 TCCGGA T^CCGGA
MunI 5818 CAATTG C^AATTG
PinAI 2007 ACCGGT A^CCGGT
PshAI 4652 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1198 CCTGCAGG CCTGCA^GG
SexAI 2813 ACCTGGT A^CC[AT]GGT
Sse8387I 1198 CCTGCAGG CCTGCA^GG
XbaI 440 TCTAGA T^CTAGA
XmnI 5562 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1214 GTAGAC GT^[AC][GT]AC
5296 GTATAC
AflIII 2755 ACGTGT A^C[GA][CT]GT
3950 ACATGT
ApaI 317 GGGCCC GGGCC^C
1319 GGGCCC
AspHI 2465 GAGCAC G[AT]GC[AT]^C
5491 GTGCAC
AspI 1190 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4920 GACGACGTC
BamHI 1712 GGATCC G^GATCC
1861 GGATCC
BbeI 1596 GGCGCC GGCGC^C
4533 GGCGCC
BclI 3439 TGATCA T^GATCA
3722 TGATCA
BsaWI 137 TCCGGA [AT]^CCGG[AT]
2007 ACCGGT
BsiHKAI 2465 GAGCAC G[AT]GC[AT]^C
5491 GTGCAC
BspMI 397 ACCTGC ACCTGC(4/8)
1604 ACCTGC
BsrDI 2086 GCAATG GCAATG(2/0)
2459 GCAATG
DraIII 507 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5091 CACAGAGTG
EcoNI 192 CCTGACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1551 CCTCGCCCAGG
FbaI 3439 TGATCA T^GATCA
3722 TGATCA
KasI 1596 GGCGCC G^GCGCC
4533 GGCGCC
NarI 1596 GGCGCC GG^CGCC
4533 GGCGCC
PflFI 1190 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4920 GACGACGTC
PpuMI 940 AGGACCT [GA]G^G[AT]CC[CT]
2881 AGGACCT
SfoI 1596 GGCGCC GGC^GCC
4533 GGCGCC
SmaI 1145 CCCGGG CCC^GGG
4585 CCCGGG
SphI 2872 GCATGC GCATG^C
5320 GCATGC
TaqI 4455 TCGA T^CGA
5035 TCGA
Tth111I 1190 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4920 GACGACGTC
TthHB8I 4455 TCGA T^CGA
5035 TCGA
XmaCI 1145 CCCGGG C^CCGGG
4585 CCCGGG
XmaI 1145 CCCGGG C^CCGGG
4585 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AseI AsnI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI PvuII RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII