##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BbvCI BciVI
BclI BfrI BsiWI BsmBI BsrFI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FbaI FseI FspI KpnI KspI
MluI NaeI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SgrAI Sse8387I SwaI Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 1748 CGCG CG^CG
AccIII 1800 TCCGGA T^CCGGA
AflIII 4052 ACATGT A^C[GA][CT]GT
AvaI 2214 CCCGGG C^[CT]CG[GA]G
BamHI 3562 GGATCC G^GATCC
BbeI 1572 GGCGCC GGCGC^C
BbrPI 2067 CACGTG CAC^GTG
BglI 5380 GCCTAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2794 AGATCT A^GATCT
BsaI 842 GAGACC GGTCTC(1/5)
BsaWI 1800 TCCGGA [AT]^CCGG[AT]
BseAI 1800 TCCGGA T^CCGGA
BsiEI 97 CGGTCG CG[GA][CT]^CG
BsmI 3958 GCATTC GAATGC(1/-1)
BsoBI 2214 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3503 TGTACA T^GTACA
BspDI 3789 ATCGAT AT^CGAT
BspEI 1800 TCCGGA T^CCGGA
BspHI 1978 TCATGA T^CATGA
BspLU11I 4052 ACATGT A^CATGT
BsrGI 3503 TGTACA T^GTACA
BssSI 1560 CTCGTG CACGAG(-5/-1)
BstUI 1748 CGCG CG^CG
BstXI 3699 CCATCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 3789 ATCGAT AT^CGAT
DraIII 3333 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 17 CCTCTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4917 GATATC GAT^ATC
EcoT22I 1643 ATGCAT ATGCA^T
HaeII 1572 GGCGCC [GA]GCGC^[CT]
KasI 1572 GGCGCC G^GCGCC
MroI 1800 TCCGGA T^CCGGA
MvnI 1748 CGCG CG^CG
NarI 1572 GGCGCC GG^CGCC
NdeI 3617 CATATG CA^TATG
NheI 1345 GCTAGC G^CTAGC
NsiI 1643 ATGCAT ATGCA^T
PmaCI 2067 CACGTG CAC^GTG
PmlI 2067 CACGTG CAC^GTG
RcaI 1978 TCATGA T^CATGA
ScaI 242 AGTACT AGT^ACT
SfoI 1572 GGCGCC GGC^GCC
SmaI 2214 CCCGGG CCC^GGG
SmlI 2306 CTCAAG C^T[CT][GA]AG
SpeI 4070 ACTAGT A^CTAGT
SphI 3475 GCATGC GCATG^C
SspBI 3503 TGTACA T^GTACA
SspI 4188 AATATT AAT^ATT
XmaCI 2214 CCCGGG C^CCGGG
XmaI 2214 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 271 GTCTAC GT^[AC][GT]AC
5070 GTCTAC
AcyI 582 GACGCC G[GA]^CG[CT]C
1572 GGCGCC
AocI 4851 CCTTAGG CC^T[ACGT]AGG
4950 CCTCAGG
Asp700I 1232 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2318 GAAAGTATTC
AspHI 342 GAGCTC G[AT]GC[AT]^C
2097 GAGCTC
AvrII 2869 CCTAGG C^CTAGG
5269 CCTAGG
BcgI 1537 CGATGTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
91 GCAGGGCGGTCG
BlnI 2869 CCTAGG C^CTAGG
5269 CCTAGG
BlpI 2369 GCTGAGC GC^T[ACGT]AGC
2374 GCTAAGC
Bpu1102I 2369 GCTGAGC GC^T[ACGT]AGC
2374 GCTAAGC
BsaBI 3443 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4316 GATTTGCATC
BsaHI 582 GACGCC G[GA]^CG[CT]C
1572 GGCGCC
BseRI 681 CTCCTC GAGGAG(10/8)
5293 CTCCTC
BsiHKAI 342 GAGCTC G[AT]GC[AT]^C
2097 GAGCTC
BsrBI 2111 GAGCGG CCGCTC(-3/-3)
2654 GAGCGG
BstBI 1140 TTCGAA TT^CGAA
2325 TTCGAA
Bsu36I 4851 CCTTAGG CC^T[ACGT]AGG
4950 CCTCAGG
CelII 2369 GCTGAGC GC^T[ACGT]AGC
2374 GCTAAGC
CfoI 1573 GCGC GCG^C
5336 GCGC
DraI 157 TTTAAA TTT^AAA
4184 TTTAAA
DraII 3363 AGGACCT [GA]G^G[ACGT]CC[CT]
5272 AGGTCCC
Eco81I 4851 CCTTAGG CC^T[ACGT]AGG
4950 CCTCAGG
EcoO109I 3363 AGGACCT [GA]G^G[ACGT]CC[CT]
5272 AGGTCCC
HhaI 1573 GCGC GCG^C
5336 GCGC
Hin1I 582 GACGCC G[GA]^CG[CT]C
1572 GGCGCC
HinP1I 1573 GCGC G^CGC
5336 GCGC
MamI 3443 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4316 GATTTGCATC
MfeI 1680 CAATTG C^AATTG
1896 CAATTG
MunI 1680 CAATTG C^AATTG
1896 CAATTG
NspV 1140 TTCGAA TT^CGAA
2325 TTCGAA
PpuMI 3363 AGGACCT [GA]G^G[AT]CC[CT]
5272 AGGTCCC
SacI 342 GAGCTC GAGCT^C
2097 GAGCTC
SfuI 1140 TTCGAA TT^CGAA
2325 TTCGAA
SnaBI 4213 TACGTA TAC^GTA
4876 TACGTA
XbaI 1357 TCTAGA T^CTAGA
2101 TCTAGA
XmnI 1232 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2318 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII