##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
Aor51HI ApaLI AscI AviII AvrII
BalI BamHI BclI BglI BlnI
BsaBI BsiEI BsiWI BsmBI BspDI
BspLU11I BspMI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I FbaI FseI FspI
HgaI HpaI KspI MamI MluI
MluNI MscI NdeI NheI NotI
NruI NspI NspV PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2603 GGTACC G^GTACC
AccIII 4920 TCCGGA T^CCGGA
AcyI 86 GGCGCC G[GA]^CG[CT]C
AhdI 4790 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 458 CCTCAGG CC^T[ACGT]AGG
AseI 4526 ATTAAT AT^TAAT
AsnI 4526 ATTAAT AT^TAAT
Asp700I 2760 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2603 GGTACC G^GTACC
AspEI 4790 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1023 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 86 GGCGCC GGCGC^C
BbrPI 447 CACGTG CAC^GTG
BciVI 906 GGATAC GTATCC(6/5)
BsaAI 447 CACGTG [CT]AC^GT[GA]
BsaHI 86 GGCGCC G[GA]^CG[CT]C
BseAI 4920 TCCGGA T^CCGGA
BspEI 4920 TCCGGA T^CCGGA
BsrDI 2598 GCAATG GCAATG(2/0)
BstXI 3451 CCAGAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 458 CCTCAGG CC^T[ACGT]AGG
Eam1105I 4790 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 458 CCTCAGG CC^T[ACGT]AGG
EcoRV 3705 GATATC GAT^ATC
Hin1I 86 GGCGCC G[GA]^CG[CT]C
KasI 86 GGCGCC G^GCGCC
KpnI 2603 GGTACC GGTAC^C
MfeI 2310 CAATTG C^AATTG
MroI 4920 TCCGGA T^CCGGA
MunI 2310 CAATTG C^AATTG
NaeI 150 GCCGGC GCC^GGC
NarI 86 GGCGCC GG^CGCC
NcoI 4257 CCATGG C^CATGG
NgoMIV 150 GCCGGC G^CCGGC
PacI 864 TTAATTAA TTAAT^TAA
PflFI 1023 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 292 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 447 CACGTG CAC^GTG
PmlI 447 CACGTG CAC^GTG
PshBI 4526 ATTAAT AT^TAAT
SexAI 1454 ACCTGGT A^CC[AT]GGT
SfoI 86 GGCGCC GGC^GCC
SpeI 3668 ACTAGT A^CTAGT
Tth111I 1023 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 292 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2760 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 20 CGCG CG^CG
67 CGCG
AflII 2514 CTTAAG C^TTAAG
4732 CTTAAG
AflIII 446 ACACGT A^C[GA][CT]GT
2320 ACGTGT
BanI 86 GGCGCC G^G[CT][GA]CC
2603 GGTACC
BcgI 3526 CGAGTGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4440 GCAGAGGTCTCG
BfrI 2514 CTTAAG C^TTAAG
4732 CTTAAG
BlpI 103 GCTGAGC GC^T[ACGT]AGC
2223 GCTGAGC
Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC
2223 GCTGAGC
BsaI 2735 GGTCTC GGTCTC(1/5)
4445 GGTCTC
BsaWI 507 ACCGGA [AT]^CCGG[AT]
4920 TCCGGA
Bsp1407I 731 TGTACA T^GTACA
2206 TGTACA
BsrBI 98 GAGCGG CCGCTC(-3/-3)
106 GAGCGG
BsrFI 150 GCCGGC [GA]^CCGG[CT]
316 ACCGGC
BsrGI 731 TGTACA T^GTACA
2206 TGTACA
BssSI 1821 CACGAG CACGAG(-5/-1)
34 CTCGTG
BstUI 20 CGCG CG^CG
67 CGCG
CelII 103 GCTGAGC GC^T[ACGT]AGC
2223 GCTGAGC
Cfr10I 150 GCCGGC [GA]^CCGG[CT]
316 ACCGGC
DraIII 475 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3523 CACCGAGTG
DrdI 1530 GACAATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2315 GACAAACGTGTC
DsaI 331 CCGTGG C^C[GA][CT]GG
4257 CCATGG
EcoRI 949 GAATTC G^AATTC
1543 GAATTC
HindIII 1400 AAGCTT A^AGCTT
4714 AAGCTT
MaeII 448 ACGT A^CGT
2320 ACGT
MvnI 20 CGCG CG^CG
67 CGCG
SapI 257 GAAGAGC GCTCTTC(1/4)
2869 GCTCTTC
SmaI 28 CCCGGG CCC^GGG
90 CCCGGG
SspBI 731 TGTACA T^GTACA
2206 TGTACA
StuI 2941 AGGCCT AGG^CCT
3540 AGGCCT
XmaCI 28 CCCGGG C^CCGGG
90 CCCGGG
XmaI 28 CCCGGG C^CCGGG
90 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII