##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I AocI ApaI ApaLI
AscI Asp718I AspHI AspI AvaI
AviII BanI BanII BbeI BbrPI
BcgI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I FseI FspI HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I StuI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 24 CGCG CG^CG
AcyI 3247 GACGCC G[GA]^CG[CT]C
AflII 3709 CTTAAG C^TTAAG
AhdI 1631 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 873 AGCGCT AGC^GCT
Asp700I 1994 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1631 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4157 CCTAGG C^CTAGG
BalI 3195 TGGCCA TGG^CCA
BamHI 1091 GGATCC G^GATCC
BbvCI 2889 GCTGAGG CCTCAGC(-5/-2)
BclI 5096 TGATCA T^GATCA
BcnI 54 CCGGG CC^[GC]GG
BfrI 3709 CTTAAG C^TTAAG
BglII 1105 AGATCT A^GATCT
BlnI 4157 CCTAGG C^CTAGG
BpmI 2034 CTCCAG CTGGAG(16/14)
BsaBI 1594 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3247 GACGCC G[GA]^CG[CT]C
BseRI 916 CTCCTC GAGGAG(10/8)
BsiEI 26 CGGTCG CG[GA][CT]^CG
BstBI 810 TTCGAA TT^CGAA
BstUI 24 CGCG CG^CG
CfoI 874 GCGC GCG^C
EaeI 3195 TGGCCA [CT]^GGCC[GA]
Eam1105I 1631 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 873 AGCGCT AGC^GCT
FbaI 5096 TGATCA T^GATCA
HaeII 873 AGCGCT [GA]GCGC^[CT]
HapII 54 CCGG C^CGG
HhaI 874 GCGC GCG^C
Hin1I 3247 GACGCC G[GA]^CG[CT]C
HinP1I 874 GCGC G^CGC
HincII 3244 GTTGAC GT[CT]^[GA]AC
HindII 3244 GTTGAC GT[CT]^[GA]AC
HpaII 54 CCGG C^CGG
MaeII 42 ACGT A^CGT
MamI 1594 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3195 TGGCCA TGG^CCA
MscI 3195 TGGCCA TGG^CCA
MspI 54 CCGG C^CGG
MvnI 24 CGCG CG^CG
NciI 54 CCGGG CC^[GC]GG
NspV 810 TTCGAA TT^CGAA
PflMI 1212 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1388 GGGTCCT [GA]G^G[AT]CC[CT]
SfuI 810 TTCGAA TT^CGAA
SwaI 3590 ATTTAAAT ATTT^AAAT
Van91I 1212 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1994 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1787 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
5057 CAGTGACTG
BbsI 1994 GAAGAC GAAGAC(2/6)
3057 GTCTTC
BciVI 3116 GTATCC GTATCC(6/5)
3386 GTATCC
BglI 2377 GCCACTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2916 GCCAGCCTGGC
BmyI 34 GTGCCC G[AGT]GC[ACT]^C
2854 GGGCAC
BpuAI 1994 GAAGAC GAAGAC(2/6)
3057 GTCTTC
Bsp1286I 34 GTGCCC G[AGT]GC[ACT]^C
2854 GGGCAC
BspMI 3679 GCAGGT ACCTGC(4/8)
4217 GCAGGT
BstAPI 537 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
641 GCAATAAATGC
BstXI 448 CCAGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
540 CCATTTGCTTGG
BstYI 1091 GGATCC [GA]^GATC[CT]
1105 AGATCT
DraII 1388 GGGTCCT [GA]G^G[ACGT]CC[CT]
4301 AGGGCCT
EcoO109I 1388 GGGTCCT [GA]G^G[ACGT]CC[CT]
4301 AGGGCCT
EcoRI 723 GAATTC G^AATTC
4933 GAATTC
EcoRV 308 GATATC GAT^ATC
3288 GATATC
FauI 49 GCGGG CCCGC(4/6)
650 GCGGG
HgaI 3247 GACGC GACGC(5/10)
467 GCGTC
HindIII 1929 AAGCTT A^AGCTT
2249 AAGCTT
MflI 1091 GGATCC [GA]^GATC[CT]
1105 AGATCT
NdeI 1266 CATATG CA^TATG
1284 CATATG
PstI 785 CTGCAG CTGCA^G
4475 CTGCAG
PvuII 2037 CAGCTG CAG^CTG
3687 CAGCTG
SexAI 121 ACCTGGT A^CC[AT]GGT
1495 ACCTGGT
XhoII 1091 GGATCC [GA]^GATC[CT]
1105 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BbvI BfaI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MfeI MnlI MseI MslI
MspA1I MunI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I