##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccI AclI AgeI AocI
Aor51HI ApaI AscI Asp718I AviII
BciVI BsiWI BspDI BssHII Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraIII Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KpnI MluI NaeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RsrII SalI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 814 GACGTC GACGT^C
AccIII 2098 TCCGGA T^CCGGA
Alw44I 4310 GTGCAC G^TGCAC
ApaLI 4310 GTGCAC G^TGCAC
AseI 4948 ATTAAT AT^TAAT
AsnI 4948 ATTAAT AT^TAAT
Asp700I 1166 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 148 CCCGAG C^[CT]CG[GA]G
AvrII 4466 CCTAGG C^CTAGG
BamHI 2228 GGATCC G^GATCC
BbeI 169 GGCGCC GGCGC^C
BbrPI 4188 CACGTG CAC^GTG
BbvCI 4390 GCTGAGG CCTCAGC(-5/-2)
BglI 959 GCCAAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4284 AGATCT A^GATCT
BlnI 4466 CCTAGG C^CTAGG
BlpI 1374 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1374 GCTCAGC GC^T[ACGT]AGC
BsaBI 3505 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4481 GAGACC GGTCTC(1/5)
BseAI 2098 TCCGGA T^CCGGA
BsmI 1207 GCATTC GAATGC(1/-1)
BsoBI 148 CCCGAG C^[CT]CG[GA]G
BspEI 2098 TCCGGA T^CCGGA
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BsrFI 134 ACCGGC [GA]^CCGG[CT]
BstAPI 315 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1773 TTCGAA TT^CGAA
BstXI 1616 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1374 GCTCAGC GC^T[ACGT]AGC
Cfr10I 134 ACCGGC [GA]^CCGG[CT]
DrdI 4290 GACATAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1814 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1253 GACGC GACGC(5/10)
KasI 169 GGCGCC G^GCGCC
KspI 1555 CCGCGG CCGC^GG
MamI 3505 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2098 TCCGGA T^CCGGA
NarI 169 GGCGCC GG^CGCC
NspV 1773 TTCGAA TT^CGAA
PmaCI 4188 CACGTG CAC^GTG
PmlI 4188 CACGTG CAC^GTG
PshBI 4948 ATTAAT AT^TAAT
SacI 1195 GAGCTC GAGCT^C
SacII 1555 CCGCGG CCGC^GG
SbfI 1349 CCTGCAGG CCTGCA^GG
ScaI 4838 AGTACT AGT^ACT
SfoI 169 GGCGCC GGC^GCC
SfuI 1773 TTCGAA TT^CGAA
Sse8387I 1349 CCTGCAGG CCTGCA^GG
SspI 4945 AATATT AAT^ATT
XmnI 1166 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 169 GGCGCC G[GA]^CG[CT]C
814 GACGTC
AflII 777 CTTAAG C^TTAAG
3229 CTTAAG
AhdI 814 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2089 GACAGATGGTC
AspEI 814 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2089 GACAGATGGTC
BbsI 1493 GAAGAC GAAGAC(2/6)
1170 GTCTTC
BcgI 536 CGAAACTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
948 GCAGCAGTCTCG
BfrI 777 CTTAAG C^TTAAG
3229 CTTAAG
BpuAI 1493 GAAGAC GAAGAC(2/6)
1170 GTCTTC
BsaAI 3092 TACGTG [CT]AC^GT[GA]
4188 CACGTG
BsaHI 169 GGCGCC G[GA]^CG[CT]C
814 GACGTC
BsiEI 75 CGGCCG CG[GA][CT]^CG
136 CGGCCG
Bsp1407I 3280 TGTACA T^GTACA
3879 TGTACA
BspHI 1249 TCATGA T^CATGA
2883 TCATGA
BspMI 1348 ACCTGC ACCTGC(4/8)
1486 ACCTGC
BsrGI 3280 TGTACA T^GTACA
3879 TGTACA
EagI 75 CGGCCG C^GGCCG
136 CGGCCG
Eam1105I 814 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2089 GACAGATGGTC
EclXI 75 CGGCCG C^GGCCG
136 CGGCCG
Eco52I 75 CGGCCG C^GGCCG
136 CGGCCG
HaeII 169 GGCGCC [GA]GCGC^[CT]
179 AGCGCC
Hin1I 169 GGCGCC G[GA]^CG[CT]C
814 GACGTC
MfeI 2736 CAATTG C^AATTG
3210 CAATTG
MunI 2736 CAATTG C^AATTG
3210 CAATTG
NdeI 1219 CATATG CA^TATG
1701 CATATG
PpuMI 327 GGGACCC [GA]G^G[AT]CC[CT]
2901 GGGACCC
RcaI 1249 TCATGA T^CATGA
2883 TCATGA
SapI 517 GAAGAGC GCTCTTC(1/4)
2602 GCTCTTC
SspBI 3280 TGTACA T^GTACA
3879 TGTACA
StuI 371 AGGCCT AGG^CCT
729 AGGCCT
XbaI 1727 TCTAGA T^CTAGA
3261 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaII BalI BanI BanII
BbvI BclI BcnI BfaI BmyI
BpmI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspLU11I BsrDI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII