##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AflII AflIII Aor51HI AscI
AseI AsnI AviII BbeI BclI
BfrI BsaBI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII BstBI
BstEII BstPI ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FbaI FseI FspI
HpaI KasI KspI MamI MfeI
MluI MunI NarI NotI NruI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfoI SfuI SgrAI SpeI Sse8387I
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2118 GACGTC GACGT^C
AccII 2195 CGCG CG^CG
AccIII 1552 TCCGGA T^CCGGA
AgeI 2536 ACCGGT A^CCGGT
AhdI 557 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4545 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 557 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1632 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3107 CCTAGG C^CTAGG
BbrPI 2042 CACGTG CAC^GTG
BbsI 2283 GAAGAC GAAGAC(2/6)
BcgI 2167 GCAAAATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3107 CCTAGG C^CTAGG
BlpI 815 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 815 GCTGAGC GC^T[ACGT]AGC
BpuAI 2283 GAAGAC GAAGAC(2/6)
BseAI 1552 TCCGGA T^CCGGA
BspEI 1552 TCCGGA T^CCGGA
BspHI 3707 TCATGA T^CATGA
BsrBI 246 GAGCGG CCGCTC(-3/-3)
BsrDI 2560 GCAATG GCAATG(2/0)
Bst1107I 4689 GTATAC GTA^TAC
BstUI 2195 CGCG CG^CG
BstZ17I 4689 GTATAC GTA^TAC
CelII 815 GCTGAGC GC^T[ACGT]AGC
DraIII 786 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 557 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5396 GAATTC G^AATTC
EcoT22I 4917 ATGCAT ATGCA^T
HindIII 4546 AAGCTT A^AGCTT
MroI 1552 TCCGGA T^CCGGA
MvnI 2195 CGCG CG^CG
NsiI 4917 ATGCAT ATGCA^T
PaeR7I 5118 CTCGAG C^TCGAG
PflFI 1632 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2536 ACCGGT A^CCGGT
PmaCI 2042 CACGTG CAC^GTG
PmlI 2042 CACGTG CAC^GTG
RcaI 3707 TCATGA T^CATGA
SalI 1060 GTCGAC G^TCGAC
SfiI 1988 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 5522 TACGTA TAC^GTA
Tth111I 1632 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5118 CTCGAG C^TCGAG
XmnI 4545 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 311 GGTACC G^GTACC
536 GGTACC
AcsI 3815 GAATTT [GA]^AATT[CT]
5396 GAATTC
AcyI 2118 GACGTC G[GA]^CG[CT]C
5084 GGCGTC
ApoI 3815 GAATTT [GA]^AATT[CT]
5396 GAATTC
Asp718I 311 GGTACC G^GTACC
536 GGTACC
BamHI 3294 GGATCC G^GATCC
4033 GGATCC
BciVI 2493 GGATAC GTATCC(6/5)
4873 GGATAC
BglII 42 AGATCT A^GATCT
4999 AGATCT
BsaAI 2042 CACGTG [CT]AC^GT[GA]
5522 TACGTA
BsaHI 2118 GACGTC G[GA]^CG[CT]C
5084 GGCGTC
BsaI 1429 GAGACC GGTCTC(1/5)
3774 GAGACC
BsmBI 1249 GAGACG CGTCTC(1/5)
1355 GAGACG
BsmI 4102 GAATGC GAATGC(1/-1)
4204 GCATTC
BstAPI 83 GCAAACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4118 GCATTGTGTGC
HaeII 1660 AGCGCC [GA]GCGC^[CT]
5155 AGCGCC
HgaI 1357 GACGC GACGC(5/10)
5085 GCGTC
Hin1I 2118 GACGTC G[GA]^CG[CT]C
5084 GGCGTC
KpnI 311 GGTACC GGTAC^C
536 GGTACC
NaeI 1452 GCCGGC GCC^GGC
1703 GCCGGC
NcoI 502 CCATGG C^CATGG
3863 CCATGG
NdeI 2266 CATATG CA^TATG
4808 CATATG
NgoMIV 1452 GCCGGC G^CCGGC
1703 GCCGGC
NheI 351 GCTAGC G^CTAGC
4084 GCTAGC
PflMI 2649 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5447 CCAACATTTGG
SacI 1950 GAGCTC GAGCT^C
5297 GAGCTC
SapI 3474 GCTCTTC GCTCTTC(1/4)
4376 GCTCTTC
ScaI 2161 AGTACT AGT^ACT
4724 AGTACT
SexAI 26 ACCTGGT A^CC[AT]GGT
787 ACCTGGT
Van91I 2649 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5447 CCAACATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI