##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 4013 GGTACC G^GTACC
AccI 3666 GTAGAC GT^[AC][GT]AC
AclI 3237 AACGTT AA^CGTT
AhdI 518 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 4013 GGTACC G^GTACC
AspEI 518 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4785 TGGCCA TGG^CCA
BbsI 1586 GTCTTC GAAGAC(2/6)
BclI 2827 TGATCA T^GATCA
BpuAI 1586 GTCTTC GAAGAC(2/6)
BsaAI 3231 CACGTA [CT]AC^GT[GA]
BsaWI 3054 ACCGGA [AT]^CCGG[AT]
BspHI 1963 TCATGA T^CATGA
BstAPI 814 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3321 CCAATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 836 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 948 GACTGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4785 TGGCCA [CT]^GGCC[GA]
Eam1105I 518 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3143 GATATC GAT^ATC
FbaI 2827 TGATCA T^GATCA
HindIII 4876 AAGCTT A^AGCTT
KpnI 4013 GGTACC GGTAC^C
MluNI 4785 TGGCCA TGG^CCA
MscI 4785 TGGCCA TGG^CCA
NdeI 1436 CATATG CA^TATG
PflMI 4068 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3237 AACGTT AA^CGTT
RcaI 1963 TCATGA T^CATGA
SapI 2368 GAAGAGC GCTCTTC(1/4)
StuI 375 AGGCCT AGG^CCT
SwaI 147 ATTTAAAT ATTT^AAAT
Van91I 4068 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 1070 CCGC CCGC(-3/-1)
4289 CCGC
AflIII 2151 ACATGT A^C[GA][CT]GT
5479 ACATGT
AlwNI 3357 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
4089 CAGTGACTG
AseI 2510 ATTAAT AT^TAAT
4780 ATTAAT
AsnI 2510 ATTAAT AT^TAAT
4780 ATTAAT
BcnI 2844 CCCGG CC^[GC]GG
3886 CCGGG
BsaBI 802 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5507 GATCTTGATC
BseRI 1257 CTCCTC GAGGAG(10/8)
4208 GAGGAG
BspLU11I 2151 ACATGT A^CATGT
5479 ACATGT
EcoRI 1773 GAATTC G^AATTC
2266 GAATTC
EcoT22I 3843 ATGCAT ATGCA^T
4354 ATGCAT
HincII 1541 GTCAAC GT[CT]^[GA]AC
3349 GTTGAC
HindII 1541 GTCAAC GT[CT]^[GA]AC
3349 GTTGAC
MamI 802 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5507 GATCTTGATC
MfeI 5097 CAATTG C^AATTG
5226 CAATTG
MspA1I 978 CAGCTG C[AC]G^C[GT]G
1890 CAGCTG
MunI 5097 CAATTG C^AATTG
5226 CAATTG
NciI 2844 CCCGG CC^[GC]GG
3886 CCGGG
NsiI 3843 ATGCAT ATGCA^T
4354 ATGCAT
PshBI 2510 ATTAAT AT^TAAT
4780 ATTAAT
PvuII 978 CAGCTG CAG^CTG
1890 CAGCTG
SmlI 1781 CTTGAG C^T[CT][GA]AG
4677 CTTGAG
SphI 322 GCATGC GCATG^C
4213 GCATGC
SspI 2402 AATATT AAT^ATT
2649 AATATT
XbaI 4057 TCTAGA T^CTAGA
5236 TCTAGA
XcmI 2586 CCATGAAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3836 CCAGTGAATGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BanI BbvI BfaI
BpmI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI