##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Aor51HI ApaI AscI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbsI BcgI BlnI BlpI
Bpu1102I BpuAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BssHII BstEII BstPI
BstUI BstXI CelII ClaI CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FauI
FseI FspI HaeII HgaI Hin1I
HindIII KasI KspI MluI MroI
MvnI NarI NcoI NheI NotI
NruI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SpeI
SphI Sse8387I Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 238 GGTACC G^GTACC
AclI 1703 AACGTT AA^CGTT
AflII 3055 CTTAAG C^TTAAG
AhdI 1416 GACCCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 500 CCTCAGG CC^T[ACGT]AGG
Asp718I 238 GGTACC G^GTACC
AspEI 1416 GACCCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3485 CACGTG CAC^GTG
BbvCI 951 CCTCAGC CCTCAGC(-5/-2)
BclI 1108 TGATCA T^GATCA
BcnI 4979 CCGGG CC^[GC]GG
BfrI 3055 CTTAAG C^TTAAG
BglI 3865 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 828 CTCCAG CTGGAG(16/14)
BsaBI 1939 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 444 GAGACC GGTCTC(1/5)
BsmFI 376 GTCCC GGGAC(10/14)
BsmI 4954 GAATGC GAATGC(1/-1)
BspHI 1525 TCATGA T^CATGA
BspLU11I 2312 ACATGT A^CATGT
BsrDI 808 CATTGC GCAATG(2/0)
BsrFI 694 GCCGGC [GA]^CCGG[CT]
BstBI 103 TTCGAA TT^CGAA
Bsu36I 500 CCTCAGG CC^T[ACGT]AGG
CfoI 1535 GCGC GCG^C
Cfr10I 694 GCCGGC [GA]^CCGG[CT]
DraII 2032 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1416 GACCCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 500 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2032 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 100 GAATTC G^AATTC
EcoT22I 2178 ATGCAT ATGCA^T
FbaI 1108 TGATCA T^GATCA
HhaI 1535 GCGC GCG^C
HinP1I 1535 GCGC G^CGC
HpaI 1431 GTTAAC GTT^AAC
KpnI 238 GGTACC GGTAC^C
MamI 1939 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 694 GCCGGC GCC^GGC
NciI 4979 CCGGG CC^[GC]GG
NdeI 4410 CATATG CA^TATG
NgoMIV 694 GCCGGC G^CCGGC
NsiI 2178 ATGCAT ATGCA^T
NspV 103 TTCGAA TT^CGAA
PacI 3126 TTAATTAA TTAAT^TAA
PflMI 5417 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3485 CACGTG CAC^GTG
PmlI 3485 CACGTG CAC^GTG
Psp1406I 1703 AACGTT AA^CGTT
PstI 600 CTGCAG CTGCA^G
RcaI 1525 TCATGA T^CATGA
SapI 717 GCTCTTC GCTCTTC(1/4)
SfuI 103 TTCGAA TT^CGAA
SnaBI 1717 TACGTA TAC^GTA
Van91I 5417 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1941 TCTAGA T^CTAGA
XcmI 5413 CCAACCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2312 ACATGT A^C[GA][CT]GT
3484 ACACGT
AlwNI 5378 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
5507 CAGGTTCTG
Asp700I 1157 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3082 GAATAACTTC
BanI 238 GGTACC G^G[CT][GA]CC
3808 GGCACC
BciVI 2661 GTATCC GTATCC(6/5)
5397 GTATCC
BssSI 792 CACGAG CACGAG(-5/-1)
2190 CACGAG
Bst1107I 1714 GTATAC GTA^TAC
4775 GTATAC
BstAPI 980 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1995 GCAATAACTGC
BstNI 584 CCTGG CC^[AT]GG
3190 CCAGG
BstZ17I 1714 GTATAC GTA^TAC
4775 GTATAC
EcoRII 584 CCTGG ^CC[AT]GG
3190 CCAGG
HapII 695 CCGG C^CGG
4979 CCGG
HpaII 695 CCGG C^CGG
4979 CCGG
MspA1I 344 CAGCGG C[AC]G^C[GT]G
380 CAGCGG
MspI 695 CCGG C^CGG
4979 CCGG
MvaI 584 CCTGG CC^[AT]GG
3190 CCAGG
ScaI 510 AGTACT AGT^ACT
2607 AGTACT
SwaI 2247 ATTTAAAT ATTT^AAAT
3504 ATTTAAAT
XmnI 1157 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3082 GAATAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI ApaLI ApoI AseI
AsnI AspHI BalI BbvI BfaI
BglII BmyI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRV EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MunI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII