##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Aor51HI AscI AseI AsnI Asp700I
AviII AvrII BamHI BbeI BbrPI
BglI BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspEI
BsrBI BssHII BssSI BstEII BstPI
BstUI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FseI
FspI Hin1I KasI KspI MamI
MluI MroI MvnI NarI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacII SalI SfiI SfoI SgrAI
SmaI SnaBI SpeI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2922 GGTACC G^GTACC
AclI 3226 AACGTT AA^CGTT
AflII 2168 CTTAAG C^TTAAG
AhdI 243 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1041 GTGCAC G^TGCAC
ApaI 3130 GGGCCC GGGCC^C
ApaLI 1041 GTGCAC G^TGCAC
Asp718I 2922 GGTACC G^GTACC
AspEI 243 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1402 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 4373 CCTCAGC CCTCAGC(-5/-2)
BcgI 4134 GCATTCCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4443 GTATCC GTATCC(6/5)
BcnI 2021 CCGGG CC^[GC]GG
BfrI 2168 CTTAAG C^TTAAG
BlpI 1053 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1053 GCTTAGC GC^T[ACGT]AGC
BsaAI 3179 TACGTG [CT]AC^GT[GA]
Bsp1407I 3539 TGTACA T^GTACA
BspDI 2928 ATCGAT AT^CGAT
BsrFI 1957 GCCGGC [GA]^CCGG[CT]
BsrGI 3539 TGTACA T^GTACA
Bst1107I 3331 GTATAC GTA^TAC
BstZ17I 3331 GTATAC GTA^TAC
CelII 1053 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1957 GCCGGC [GA]^CCGG[CT]
ClaI 2928 ATCGAT AT^CGAT
DrdI 481 GACTATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 243 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 2495 GGCGCT [GA]GCGC^[CT]
HpaI 4845 GTTAAC GTT^AAC
KpnI 2922 GGTACC GGTAC^C
NaeI 1957 GCCGGC GCC^GGC
NciI 2021 CCGGG CC^[GC]GG
NgoMIV 1957 GCCGGC G^CCGGC
PflFI 1402 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1345 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3226 AACGTT AA^CGTT
SapI 3969 GCTCTTC GCTCTTC(1/4)
SbfI 3389 CCTGCAGG CCTGCA^GG
SphI 3384 GCATGC GCATG^C
Sse8387I 3389 CCTGCAGG CCTGCA^GG
SspBI 3539 TGTACA T^GTACA
SwaI 4886 ATTTAAAT ATTT^AAAT
Tth111I 1402 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4497 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 3191 GTCTAC GT^[AC][GT]AC
3331 GTATAC
AocI 1082 CCTGAGG CC^T[ACGT]AGG
3357 CCTGAGG
BalI 4006 TGGCCA TGG^CCA
4665 TGGCCA
BanI 1960 GGCACC G^G[CT][GA]CC
2922 GGTACC
BclI 745 TGATCA T^GATCA
2459 TGATCA
BglII 151 AGATCT A^GATCT
4395 AGATCT
BsaI 1407 GGTCTC GGTCTC(1/5)
3657 GGTCTC
BstBI 447 TTCGAA TT^CGAA
2386 TTCGAA
BstXI 1860 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2788 CCACGACCATGG
Bsu36I 1082 CCTGAGG CC^T[ACGT]AGG
3357 CCTGAGG
CfoI 196 GCGC GCG^C
2496 GCGC
DsaI 517 CCATGG C^C[GA][CT]GG
2794 CCATGG
EaeI 4006 TGGCCA [CT]^GGCC[GA]
4665 TGGCCA
EarI 1410 CTCTTC CTCTTC(1/4)
3970 CTCTTC
Eco81I 1082 CCTGAGG CC^T[ACGT]AGG
3357 CCTGAGG
EcoRI 641 GAATTC G^AATTC
3122 GAATTC
EcoT22I 3309 ATGCAT ATGCA^T
4426 ATGCAT
FbaI 745 TGATCA T^GATCA
2459 TGATCA
HapII 1958 CCGG C^CGG
2021 CCGG
HgaI 2523 GCGTC GACGC(5/10)
4494 GCGTC
HhaI 196 GCGC GCG^C
2496 GCGC
HinP1I 196 GCGC G^CGC
2496 GCGC
HindIII 2641 AAGCTT A^AGCTT
2818 AAGCTT
HpaII 1958 CCGG C^CGG
2021 CCGG
Ksp632I 1410 CTCTTC CTCTTC(1/4)
3970 CTCTTC
MluNI 4006 TGGCCA TGG^CCA
4665 TGGCCA
MscI 4006 TGGCCA TGG^CCA
4665 TGGCCA
MspI 1958 CCGG C^CGG
2021 CCGG
NcoI 517 CCATGG C^CATGG
2794 CCATGG
NdeI 2070 CATATG CA^TATG
3723 CATATG
NsiI 3309 ATGCAT ATGCA^T
4426 ATGCAT
NspV 447 TTCGAA TT^CGAA
2386 TTCGAA
PpuMI 1078 AGGACCT [GA]G^G[AT]CC[CT]
1120 GGGTCCT
PstI 3390 CTGCAG CTGCA^G
3934 CTGCAG
SacI 384 GAGCTC GAGCT^C
522 GAGCTC
ScaI 1070 AGTACT AGT^ACT
2154 AGTACT
SexAI 856 ACCTGGT A^CC[AT]GGT
4088 ACCAGGT
SfuI 447 TTCGAA TT^CGAA
2386 TTCGAA
StuI 3394 AGGCCT AGG^CCT
3619 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII