##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI Aor51HI ApaI AscI
Asp718I AviII AvrII BanI BbeI
BbrPI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII CfoI
Cfr10I CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmlI PshAI
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2513 GTCTAC GT^[AC][GT]AC
AflIII 984 ACATGT A^C[GA][CT]GT
AocI 5138 CCTGAGG CC^T[ACGT]AGG
AseI 4510 ATTAAT AT^TAAT
AsnI 4510 ATTAAT AT^TAAT
AspI 5351 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5303 CCCGAG C^[CT]CG[GA]G
BamHI 2238 GGATCC G^GATCC
BanII 1659 GAGCCC G[GA]GC[CT]^C
BbvCI 5255 GCTGAGG CCTCAGC(-5/-2)
BciVI 2342 GGATAC GTATCC(6/5)
BcnI 284 CCGGG CC^[GC]GG
BsmFI 3634 GGGAC GGGAC(10/14)
BsoBI 5303 CCCGAG C^[CT]CG[GA]G
BspDI 2585 ATCGAT AT^CGAT
BspLU11I 984 ACATGT A^CATGT
BstEII 4498 GGTTACC G^GT[ACGT]ACC
BstPI 4498 GGTTACC G^GT[ACGT]ACC
Bsu36I 5138 CCTGAGG CC^T[ACGT]AGG
ClaI 2585 ATCGAT AT^CGAT
DraII 3634 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 5138 CCTGAGG CC^T[ACGT]AGG
EcoO109I 3634 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 4498 GGTTACC G^GT[ACGT]ACC
EcoRI 2450 GAATTC G^AATTC
EcoRV 460 GATATC GAT^ATC
HapII 284 CCGG C^CGG
HindIII 3814 AAGCTT A^AGCTT
HpaI 1422 GTTAAC GTT^AAC
HpaII 284 CCGG C^CGG
MspI 284 CCGG C^CGG
NciI 284 CCGGG CC^[GC]GG
NdeI 513 CATATG CA^TATG
PflFI 5351 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 580 GTTTAAAC GTTT^AAAC
PpuMI 3634 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 4510 ATTAAT AT^TAAT
PstI 5020 CTGCAG CTGCA^G
SexAI 1192 ACCTGGT A^CC[AT]GGT
SpeI 256 ACTAGT A^CTAGT
StuI 1723 AGGCCT AGG^CCT
Tth111I 5351 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AclI 660 AACGTT AA^CGTT
1919 AACGTT
AhdI 1325 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2505 GACTCACAGTC
Alw44I 1885 GTGCAC G^TGCAC
1979 GTGCAC
AlwNI 2755 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
3358 CAGCATCTG
ApaLI 1885 GTGCAC G^TGCAC
1979 GTGCAC
AspEI 1325 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2505 GACTCACAGTC
AspHI 1885 GTGCAC G[AT]GC[AT]^C
1979 GTGCAC
BseRI 3 CTCCTC GAGGAG(10/8)
4099 GAGGAG
BsiHKAI 1885 GTGCAC G[AT]GC[AT]^C
1979 GTGCAC
BsmBI 5185 CGTCTC CGTCTC(1/5)
5356 CGTCTC
BsmI 1636 GAATGC GAATGC(1/-1)
951 GCATTC
BspHI 3197 TCATGA T^CATGA
3872 TCATGA
Cac8I 2546 GCAGGC GC[ACGT]^[ACGT]GC
4107 GCTCGC
Eam1105I 1325 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2505 GACTCACAGTC
HincII 116 GTCAAC GT[CT]^[GA]AC
1422 GTTAAC
HindII 116 GTCAAC GT[CT]^[GA]AC
1422 GTTAAC
PflMI 1641 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4595 CCAAAGGGTGG
Psp1406I 660 AACGTT AA^CGTT
1919 AACGTT
RcaI 3197 TCATGA T^CATGA
3872 TCATGA
Van91I 1641 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4595 CCAAAGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BalI BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cfr13I DdeI DpnII DraI DraIII
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII XmnI