##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BclI
BfaI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I RcaI RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 1980 CTTAAG C^TTAAG
AflIII 891 ACACGT A^C[GA][CT]GT
AocI 1160 CCTGAGG CC^T[ACGT]AGG
AseI 2208 ATTAAT AT^TAAT
AsnI 2208 ATTAAT AT^TAAT
AspI 1147 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 558 TGGCCA TGG^CCA
BanI 973 GGTGCC G^G[CT][GA]CC
BcgI 1183 GCAGAGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1980 CTTAAG C^TTAAG
BsaAI 892 CACGTA [CT]AC^GT[GA]
BsaI 1188 GGTCTC GGTCTC(1/5)
BsiEI 415 CGATCG CG[GA][CT]^CG
BsrDI 610 CATTGC GCAATG(2/0)
BstXI 823 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1160 CCTGAGG CC^T[ACGT]AGG
DrdI 590 GACGAGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 558 TGGCCA [CT]^GGCC[GA]
Eco81I 1160 CCTGAGG CC^T[ACGT]AGG
EcoRI 1385 GAATTC G^AATTC
HindIII 1983 AAGCTT A^AGCTT
MluNI 558 TGGCCA TGG^CCA
MscI 558 TGGCCA TGG^CCA
MslI 1762 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 2160 GCATGT [GA]CATG^[CT]
PflFI 1147 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 403 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2208 ATTAAT AT^TAAT
PvuI 415 CGATCG CGAT^CG
SapI 61 GAAGAGC GCTCTTC(1/4)
SbfI 1261 CCTGCAGG CCTGCA^GG
SexAI 1293 ACCTGGT A^CC[AT]GGT
Sse8387I 1261 CCTGCAGG CCTGCA^GG
TaqI 480 TCGA T^CGA
Tth111I 1147 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 480 TCGA T^CGA
Van91I 403 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 561 CCAAGCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1385 GAATTC [GA]^AATT[CT]
1660 AAATTT
Alw44I 383 GTGCAC G^TGCAC
544 GTGCAC
AlwI 1750 GATCC GGATC(4/5)
187 GGATC
ApaLI 383 GTGCAC G^TGCAC
544 GTGCAC
ApoI 1385 GAATTC [GA]^AATT[CT]
1660 AAATTT
Asp700I 287 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2087 GAATGTATTC
BbsI 1252 GAAGAC GAAGAC(2/6)
1894 GAAGAC
BglI 1259 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1929 GCCATGGAGGC
BpuAI 1252 GAAGAC GAAGAC(2/6)
1894 GAAGAC
BstYI 187 GGATCT [GA]^GATC[CT]
1749 AGATCC
EcoNI 1348 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1813 CCTGCTGCAGG
HaeII 1270 GGCGCT [GA]GCGC^[CT]
1922 AGCGCC
HgaI 1282 GACGC GACGC(5/10)
495 GCGTC
MflI 187 GGATCT [GA]^GATC[CT]
1749 AGATCC
MspA1I 407 CAGCTG C[AC]G^C[GT]G
784 CAGCTG
PvuII 407 CAGCTG CAG^CTG
784 CAGCTG
SmlI 1889 CTCAAG C^T[CT][GA]AG
1980 CTTAAG
TfiI 701 GAATC G^A[AT]TC
1032 GATTC
XhoII 187 GGATCT [GA]^GATC[CT]
1749 AGATCC
XmnI 287 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2087 GAATGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanII BbvI
BciVI BcnI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XmaCI
XmaI