##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AviII AvrII BbeI BcgI
BfrI BglI BlnI BsaBI BseAI
BsiEI BsiWI BsmBI BspEI BspMI
BsrFI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I Cfr10I CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FseI FspI
HaeII KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NgoMIV NotI NruI
NsiI PacI PflMI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Van91I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1147 GACGTC GACGT^C
AccII 4268 CGCG CG^CG
AcyI 1147 GACGTC G[GA]^CG[CT]C
BalI 1767 TGGCCA TGG^CCA
BamHI 1537 GGATCC G^GATCC
BbrPI 3684 CACGTG CAC^GTG
BbvCI 3920 CCTCAGC CCTCAGC(-5/-2)
BciVI 4169 GGATAC GTATCC(6/5)
BcnI 4361 CCGGG CC^[GC]GG
BlpI 4061 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4061 GCTAAGC GC^T[ACGT]AGC
BsaHI 1147 GACGTC G[GA]^CG[CT]C
BsaWI 75 ACCGGA [AT]^CCGG[AT]
BsgI 4254 GTGCAG GTGCAG(16/14)
Bsp1407I 4963 TGTACA T^GTACA
BspDI 3843 ATCGAT AT^CGAT
BsrDI 2197 GCAATG GCAATG(2/0)
BsrGI 4963 TGTACA T^GTACA
BssSI 4311 CACGAG CACGAG(-5/-1)
BstUI 4268 CGCG CG^CG
CelII 4061 GCTAAGC GC^T[ACGT]AGC
ClaI 3843 ATCGAT AT^CGAT
DraIII 1562 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4034 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3857 CCACGG C^C[GA][CT]GG
EcoRV 3797 GATATC GAT^ATC
HgaI 4034 GACGC GACGC(5/10)
Hin1I 1147 GACGTC G[GA]^CG[CT]C
HincII 89 GTTAAC GT[CT]^[GA]AC
HindII 89 GTTAAC GT[CT]^[GA]AC
HpaI 89 GTTAAC GTT^AAC
MluNI 1767 TGGCCA TGG^CCA
MscI 1767 TGGCCA TGG^CCA
MvnI 4268 CGCG CG^CG
NciI 4361 CCGGG CC^[GC]GG
PmaCI 3684 CACGTG CAC^GTG
PmlI 3684 CACGTG CAC^GTG
PshAI 3579 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 80 ACCAGGT A^CC[AT]GGT
SpeI 3066 ACTAGT A^CTAGT
SspBI 4963 TGTACA T^GTACA
StuI 5079 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1792 GTAGAC GT^[AC][GT]AC
2339 GTCTAC
ApaI 2523 GGGCCC GGGCC^C
4605 GGGCCC
AseI 907 ATTAAT AT^TAAT
4121 ATTAAT
AsnI 907 ATTAAT AT^TAAT
4121 ATTAAT
Asp700I 3375 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3464 GAATAGGTTC
AvaI 1479 CTCGAG C^[CT]CG[GA]G
1888 CTCGAG
BanI 940 GGTGCC G^G[CT][GA]CC
3144 GGTGCC
BbsI 3741 GAAGAC GAAGAC(2/6)
4671 GAAGAC
BglII 357 AGATCT A^GATCT
4162 AGATCT
BpuAI 3741 GAAGAC GAAGAC(2/6)
4671 GAAGAC
BsaAI 302 TACGTG [CT]AC^GT[GA]
3684 CACGTG
BsmI 3489 GAATGC GAATGC(1/-1)
59 GCATTC
BsoBI 1479 CTCGAG C^[CT]CG[GA]G
1888 CTCGAG
BsrBI 447 CCGCTC CCGCTC(-3/-3)
1664 GAGCGG
BstAPI 934 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3362 GCAGGGGCTGC
BstBI 615 TTCGAA TT^CGAA
3382 TTCGAA
BstXI 1711 CCAAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3747 CCAGACAAGTGG
EaeI 129 TGGCCG [CT]^GGCC[GA]
1767 TGGCCA
EcoNI 4299 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4574 CCTTGTAAAGG
FauI 1666 GCGGG CCCGC(4/6)
3650 GCGGG
HapII 76 CCGG C^CGG
4361 CCGG
HpaII 76 CCGG C^CGG
4361 CCGG
MspI 76 CCGG C^CGG
4361 CCGG
NdeI 2369 CATATG CA^TATG
2703 CATATG
NheI 2588 GCTAGC G^CTAGC
2734 GCTAGC
NspV 615 TTCGAA TT^CGAA
3382 TTCGAA
PaeR7I 1479 CTCGAG C^TCGAG
1888 CTCGAG
PshBI 907 ATTAAT AT^TAAT
4121 ATTAAT
PstI 2214 CTGCAG CTGCA^G
3369 CTGCAG
SacI 1547 GAGCTC GAGCT^C
3699 GAGCTC
SfuI 615 TTCGAA TT^CGAA
3382 TTCGAA
SphI 492 GCATGC GCATG^C
1640 GCATGC
XhoI 1479 CTCGAG C^TCGAG
1888 CTCGAG
XmnI 3375 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3464 GAATAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AspI AvaII BanII BbvI
BclI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspLU11I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII