##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Aor51HI     ApaI        AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BciVI       BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstUI       BstZ17I     CelII       CfoI        ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     FauI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SpeI        Sse8387I    
SspBI       Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

Alw44I       2657  GTGCAC               G^TGCAC
AocI          319  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2657  GTGCAC               G^TGCAC
AvaI         1181  CCCGAG               C^[CT]CG[GA]G
BanI         3184  GGTGCC               G^G[CT][GA]CC
BbsI         3485  GAAGAC               GAAGAC(2/6)
BbvCI        1172  CCTCAGC              CCTCAGC(-5/-2)
BcgI         3087  CGAAAAATTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         2429  CCGGG                CC^[GC]GG
BglII        2961  AGATCT               A^GATCT
BpuAI        3485  GAAGAC               GAAGAC(2/6)
BsaAI        4232  TACGTA               [CT]AC^GT[GA]
BsaI         4428  GGTCTC               GGTCTC(1/5)
BsoBI        1181  CCCGAG               C^[CT]CG[GA]G
BspMI         766  GCAGGT               ACCTGC(4/8)
BsrFI        2373  ACCGGC               [GA]^CCGG[CT]
BstXI        1323  CCAAAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        319  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       2373  ACCGGC               [GA]^CCGG[CT]
Eco81I        319  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1285  GAATTC               G^AATTC
EcoRV        2681  GATATC               GAT^ATC
NciI         2429  CCGGG                CC^[GC]GG
SacI         4522  GAGCTC               GAGCT^C
SapI          107  GAAGAGC              GCTCTTC(1/4)
ScaI          214  AGTACT               AGT^ACT
SnaBI        4232  TACGTA               TAC^GTA
StuI          316  AGGCCT               AGG^CCT
SwaI         4566  ATTTAAAT             ATTT^AAAT
XbaI         3133  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI          511  GCGG                 CCGC(-3/-1)
             3236  GCGG                
Asp700I       439  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2433  GAATGACTTC          
AvaII        2258  GGACC                G^G[AT]CC
             3544  GGTCC               
BalI         1269  TGGCCA               TGG^CCA
             4400  TGGCCA              
BanII         110  GAGCCC               G[GA]GC[CT]^C
             4522  GAGCTC              
BsgI         1110  GTGCAG               GTGCAG(16/14)
             2337  GTGCAG              
BsmI          592  GCATTC               GAATGC(1/-1)
             2629  GCATTC              
BspLU11I     2403  ACATGT               A^CATGT
             4910  ACATGT              
BstAPI       1136  GCAACCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2741  GCAAATTGTGC         
BstBI         148  TTCGAA               TT^CGAA
             3264  TTCGAA              
Cfr13I       2258  GGACC                G^G[ACGT]CC
             3544  GGTCC               
DraII        2257  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3543  GGGTCCC             
DraIII        574  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3549  CACATGGTG           
DsaI          506  CCATGG               C^C[GA][CT]GG
             2782  CCATGG              
EaeI         1269  TGGCCA               [CT]^GGCC[GA]
             4400  TGGCCA              
EcoO109I     2257  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3543  GGGTCCC             
HindIII      2394  AAGCTT               A^AGCTT
             4360  AAGCTT              
HpaI         1965  GTTAAC               GTT^AAC
             4995  GTTAAC              
MluNI        1269  TGGCCA               TGG^CCA
             4400  TGGCCA              
MscI         1269  TGGCCA               TGG^CCA
             4400  TGGCCA              
MwoI         1136  GCAACCTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             2741  GCAAATTGTGC         
NcoI          506  CCATGG               C^CATGG
             2782  CCATGG              
NspV          148  TTCGAA               TT^CGAA
             3264  TTCGAA              
PpuMI        2257  AGGACCT              [GA]G^G[AT]CC[CT]
             3543  GGGTCCC             
Sau96I       2258  GGACC                G^G[ACGT]CC
             3544  GGTCC               
SfuI          148  TTCGAA               TT^CGAA
             3264  TTCGAA              
SphI          939  GCATGC               GCATG^C
             3384  GCATGC              
XcmI         2875  CCAGTCAGCTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3455  CCAGTTATTCAGTGG     
XmnI          439  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2433  GAATGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        AspHI       
BbvI        BclI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
DdeI        DpnII       DraI        EarI        EcoRII      
EcoT14I     EcoT22I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        NdeI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PflMI       PleI        PshBI       
PvuII       RsaI        Sau3AI      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII