##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AgeI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BglII       BlnI        BsaAI       BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BsrGI       
BssHII      Bst1107I    BstXI       BstZ17I     ClaI        
CpoI        DraIII      EagI        EclXI       Eco47III    
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
FspI        HpaI        KasI        KpnI        MamI        
MfeI        MluI        MunI        NarI        NotI        
NruI        NsiI        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RsrII       SalI        ScaI        SfiI        SfoI        
SnaBI       SpeI        SspBI       SwaI        XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII       2021  TCCGGA               T^CCGGA
AclI         5002  AACGTT               AA^CGTT
AcyI          236  GACGCC               G[GA]^CG[CT]C
AflII        2038  CTTAAG               C^TTAAG
AhdI         2361  GACTCGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        965  GTGCAC               G^TGCAC
ApaLI         965  GTGCAC               G^TGCAC
AspEI        2361  GACTCGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         3218  TGGCCA               TGG^CCA
BamHI         558  GGATCC               G^GATCC
BanI          126  GGCACC               G^G[CT][GA]CC
BfrI         2038  CTTAAG               C^TTAAG
BsaHI         236  GACGCC               G[GA]^CG[CT]C
BsaWI        2021  TCCGGA               [AT]^CCGG[AT]
BseAI        2021  TCCGGA               T^CCGGA
BsmBI        2281  GAGACG               CGTCTC(1/5)
BspEI        2021  TCCGGA               T^CCGGA
BspHI        4517  TCATGA               T^CATGA
BspMI        4904  GCAGGT               ACCTGC(4/8)
BsrDI        3681  CATTGC               GCAATG(2/0)
BssSI        3730  CTCGTG               CACGAG(-5/-1)
BstBI         858  TTCGAA               TT^CGAA
BstEII       3822  GGTGACC              G^GT[ACGT]ACC
BstPI        3822  GGTGACC              G^GT[ACGT]ACC
DraI         5039  TTTAAA               TTT^AAA
DrdI          160  GACCATGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2361  GACTCGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3822  GGTGACC              G^GT[ACGT]ACC
Hin1I         236  GACGCC               G[GA]^CG[CT]C
KspI          955  CCGCGG               CCGC^GG
MluNI        3218  TGGCCA               TGG^CCA
MroI         2021  TCCGGA               T^CCGGA
MscI         3218  TGGCCA               TGG^CCA
NaeI         2324  GCCGGC               GCC^GGC
NcoI          162  CCATGG               C^CATGG
NdeI         1054  CATATG               CA^TATG
NgoMIV       2324  GCCGGC               G^CCGGC
NheI          591  GCTAGC               G^CTAGC
NspV          858  TTCGAA               TT^CGAA
Psp1406I     5002  AACGTT               AA^CGTT
RcaI         4517  TCATGA               T^CATGA
SacII         955  CCGCGG               CCGC^GG
SapI         4360  GCTCTTC              GCTCTTC(1/4)
SbfI         2880  CCTGCAGG             CCTGCA^GG
SexAI        3107  ACCAGGT              A^CC[AT]GGT
SfuI          858  TTCGAA               TT^CGAA
SgrAI        2323  CGCCGGCG             C[GA]^CCGG[CT]G
Sse8387I     2880  CCTGCAGG             CCTGCA^GG
SspI         5048  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          263  GTAC                 GT^AC
             1680  GTAC                
AspI          928  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3658  GACAGTGTC           
BsmI         2460  GAATGC               GAATGC(1/-1)
             3811  GCATTC              
BsrFI        2324  GCCGGC               [GA]^CCGG[CT]
             4920  GCCGGT              
Cfr10I       2324  GCCGGC               [GA]^CCGG[CT]
             4920  GCCGGT              
EcoRI        2317  GAATTC               G^AATTC
             3652  GAATTC              
HincII        677  GTCAAC               GT[CT]^[GA]AC
             1605  GTTGAC              
HindII        677  GTCAAC               GT[CT]^[GA]AC
             1605  GTTGAC              
HindIII      3593  AAGCTT               A^AGCTT
             4065  AAGCTT              
PflFI         928  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3658  GACAGTGTC           
RsaI          263  GTAC                 GT^AC
             1680  GTAC                
SphI          581  GCATGC               GCATG^C
             1712  GCATGC              
StuI          530  AGGCCT               AGG^CCT
             3764  AGGCCT              
Tth111I       928  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3658  GACAGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AflIII      
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        AspHI       AvaI        AvaII       BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspLU11I    BsrBI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PaeR7I      PflMI       PleI        PpuMI       PstI        
PvuII       SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoI        XhoII       XmaCI       XmaI