##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AclI AcyI AgeI
Alw44I Aor51HI ApaLI AscI AviII
AvrII BbeI BbrPI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsaHI
BsiWI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI FseI FspI
HaeII Hin1I HpaI KasI KspI
MluI NarI NheI NotI NruI
NspV PacI PinAI PmaCI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SpeI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 356 GGTACC G^GTACC
AccII 5298 CGCG CG^CG
AccIII 2188 TCCGGA T^CCGGA
AflII 515 CTTAAG C^TTAAG
AhdI 2037 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2109 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 356 GGTACC G^GTACC
AspEI 2037 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1440 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 356 GGTACC G^G[CT][GA]CC
BclI 1382 TGATCA T^GATCA
BfrI 515 CTTAAG C^TTAAG
BsaAI 2663 TACGTA [CT]AC^GT[GA]
BsaBI 1220 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2188 TCCGGA [AT]^CCGG[AT]
BseAI 2188 TCCGGA T^CCGGA
BsmBI 2067 GAGACG CGTCTC(1/5)
BspEI 2188 TCCGGA T^CCGGA
BspHI 4734 TCATGA T^CATGA
BsrBI 1997 GAGCGG CCGCTC(-3/-3)
BsrDI 2011 GCAATG GCAATG(2/0)
BstUI 5298 CGCG CG^CG
BstXI 230 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 198 GCGC GCG^C
DraIII 1827 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4860 CCATGG C^C[GA][CT]GG
Eam1105I 2037 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 372 CTCTTC CTCTTC(1/4)
EcoRV 3982 GATATC GAT^ATC
FbaI 1382 TGATCA T^GATCA
HgaI 2948 GACGC GACGC(5/10)
HhaI 198 GCGC GCG^C
HinP1I 198 GCGC G^CGC
HincII 4642 GTCAAC GT[CT]^[GA]AC
HindII 4642 GTCAAC GT[CT]^[GA]AC
KpnI 356 GGTACC GGTAC^C
Ksp632I 372 CTCTTC CTCTTC(1/4)
MamI 1220 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2188 TCCGGA T^CCGGA
MspA1I 2145 CAGCTG C[AC]G^C[GT]G
MvnI 5298 CGCG CG^CG
NcoI 4860 CCATGG C^CATGG
PaeR7I 1126 CTCGAG C^TCGAG
PflFI 1440 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 886 GTTTAAAC GTTT^AAAC
PpuMI 289 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 2145 CAGCTG CAG^CTG
RcaI 4734 TCATGA T^CATGA
SmaI 174 CCCGGG CCC^GGG
SnaBI 2663 TACGTA TAC^GTA
SphI 5603 GCATGC GCATG^C
StuI 2681 AGGCCT AGG^CCT
Tth111I 1440 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2570 TCTAGA T^CTAGA
XhoI 1126 CTCGAG C^TCGAG
XmaCI 174 CCCGGG C^CCGGG
XmaI 174 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 2542 ACATGT A^C[GA][CT]GT
5256 ACATGT
AocI 1161 CCTGAGG CC^T[ACGT]AGG
3154 CCTCAGG
ApaI 177 GGGCCC GGGCC^C
2135 GGGCCC
AspHI 610 GAGCAC G[AT]GC[AT]^C
2986 GTGCTC
BalI 4548 TGGCCA TGG^CCA
4941 TGGCCA
BamHI 381 GGATCC G^GATCC
1605 GGATCC
BciVI 5235 GGATAC GTATCC(6/5)
2284 GTATCC
BsaI 1729 GAGACC GGTCTC(1/5)
746 GGTCTC
BsiEI 644 CGGTCG CG[GA][CT]^CG
1568 CGACCG
BsiHKAI 610 GAGCAC G[AT]GC[AT]^C
2986 GTGCTC
BsmI 3846 GCATTC GAATGC(1/-1)
5415 GCATTC
BspLU11I 2542 ACATGT A^CATGT
5256 ACATGT
BspMI 270 GCAGGT ACCTGC(4/8)
1367 GCAGGT
BstAPI 2002 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2951 GCACACGATGC
BstEII 1939 GGTCACC G^GT[ACGT]ACC
3867 GGTAACC
BstPI 1939 GGTCACC G^GT[ACGT]ACC
3867 GGTAACC
Bsu36I 1161 CCTGAGG CC^T[ACGT]AGG
3154 CCTCAGG
EaeI 4548 TGGCCA [CT]^GGCC[GA]
4941 TGGCCA
Eco81I 1161 CCTGAGG CC^T[ACGT]AGG
3154 CCTCAGG
EcoO65I 1939 GGTCACC G^GT[ACGT]ACC
3867 GGTAACC
EcoRI 1488 GAATTC G^AATTC
2440 GAATTC
HindIII 341 AAGCTT A^AGCTT
428 AAGCTT
MfeI 2260 CAATTG C^AATTG
3973 CAATTG
MluNI 4548 TGGCCA TGG^CCA
4941 TGGCCA
MscI 4548 TGGCCA TGG^CCA
4941 TGGCCA
MunI 2260 CAATTG C^AATTG
3973 CAATTG
NaeI 186 GCCGGC GCC^GGC
266 GCCGGC
NdeI 535 CATATG CA^TATG
5461 CATATG
NgoMIV 186 GCCGGC G^CCGGC
266 GCCGGC
PflMI 1826 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2108 CCAGACTCTGG
ScaI 3434 AGTACT AGT^ACT
4514 AGTACT
SfcI 1109 CTGTAG C^T[GA][CT]AG
3987 CTGTAG
Van91I 1826 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2108 CCAGACTCTGG
XcmI 676 CCAGACTCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4837 CCACCTCTCGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI DraII EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI