##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI BcgI
BfrI BglII BsiWI BsmI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I DrdI Eam1105I EcoO65I EcoRI
EcoT22I FseI HincII HindII HindIII
HpaI MfeI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3796 GGTACC G^GTACC
AccI 3693 GTCTAC GT^[AC][GT]AC
AccIII 3219 TCCGGA T^CCGGA
AcsI 2450 GAATTT [GA]^AATT[CT]
Alw44I 4372 GTGCAC G^TGCAC
Aor51HI 2596 AGCGCT AGC^GCT
ApaLI 4372 GTGCAC G^TGCAC
ApoI 2450 GAATTT [GA]^AATT[CT]
Asp718I 3796 GGTACC G^GTACC
AspI 4784 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 939 TGCGCA TGC^GCA
BbeI 3822 GGCGCC GGCGC^C
BbsI 620 GAAGAC GAAGAC(2/6)
BciVI 1395 GTATCC GTATCC(6/5)
BclI 4290 TGATCA T^GATCA
BpuAI 620 GAAGAC GAAGAC(2/6)
BsaWI 3219 TCCGGA [AT]^CCGG[AT]
BseAI 3219 TCCGGA T^CCGGA
BsiEI 656 CGGCCG CG[GA][CT]^CG
BspEI 3219 TCCGGA T^CCGGA
BsrDI 2876 CATTGC GCAATG(2/0)
BsrFI 1961 GCCGGT [GA]^CCGG[CT]
BstAPI 1375 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1961 GCCGGT [GA]^CCGG[CT]
CpoI 1929 CGGACCG CG^G[AT]CCG
DraIII 843 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 656 CGGCCG C^GGCCG
EclXI 656 CGGCCG C^GGCCG
Eco47III 2596 AGCGCT AGC^GCT
Eco52I 656 CGGCCG C^GGCCG
EcoRV 3936 GATATC GAT^ATC
FbaI 4290 TGATCA T^GATCA
FspI 939 TGCGCA TGC^GCA
KasI 3822 GGCGCC G^GCGCC
KpnI 3796 GGTACC GGTAC^C
MluI 1536 ACGCGT A^CGCGT
MroI 3219 TCCGGA T^CCGGA
NarI 3822 GGCGCC GG^CGCC
PflFI 4784 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1929 CGGACCG CG^G[AT]CCG
SfoI 3822 GGCGCC GGC^GCC
SphI 1935 GCATGC GCATG^C
Tth111I 4784 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 557 GACGCC G[GA]^CG[CT]C
3822 GGCGCC
AocI 757 CCTGAGG CC^T[ACGT]AGG
2766 CCTGAGG
AvaI 1727 CTCGGG C^[CT]CG[GA]G
1797 CTCGGG
BamHI 1500 GGATCC G^GATCC
4168 GGATCC
BbrPI 2055 CACGTG CAC^GTG
2098 CACGTG
BsaBI 298 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
964 GATCAAGATC
BsaHI 557 GACGCC G[GA]^CG[CT]C
3822 GGCGCC
BsmBI 21 CGTCTC CGTCTC(1/5)
3260 CGTCTC
BsoBI 1727 CTCGGG C^[CT]CG[GA]G
1797 CTCGGG
Bsp1407I 148 TGTACA T^GTACA
3378 TGTACA
BspDI 158 ATCGAT AT^CGAT
1034 ATCGAT
BspHI 2150 TCATGA T^CATGA
3102 TCATGA
BspLU11I 3764 ACATGT A^CATGT
4368 ACATGT
BsrBI 1547 GAGCGG CCGCTC(-3/-3)
2891 GAGCGG
BsrGI 148 TGTACA T^GTACA
3378 TGTACA
Bsu36I 757 CCTGAGG CC^T[ACGT]AGG
2766 CCTGAGG
ClaI 158 ATCGAT AT^CGAT
1034 ATCGAT
Eco81I 757 CCTGAGG CC^T[ACGT]AGG
2766 CCTGAGG
Hin1I 557 GACGCC G[GA]^CG[CT]C
3822 GGCGCC
KspI 33 CCGCGG CCGC^GG
74 CCGCGG
MamI 298 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
964 GATCAAGATC
PmaCI 2055 CACGTG CAC^GTG
2098 CACGTG
PmlI 2055 CACGTG CAC^GTG
2098 CACGTG
PpuMI 2550 GGGTCCT [GA]G^G[AT]CC[CT]
3663 AGGACCC
RcaI 2150 TCATGA T^CATGA
3102 TCATGA
SacII 33 CCGCGG CCGC^GG
74 CCGCGG
SapI 4325 GAAGAGC GCTCTTC(1/4)
429 GCTCTTC
SspBI 148 TGTACA T^GTACA
3378 TGTACA
Tsp509I 2451 AATT ^AATT
4706 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI Asp700I AspHI
AvaII AvrII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmnI