##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Aor51HI AscI Asp718I AviII BamHI
BcgI BfrI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoRV FseI FspI KpnI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SalI SapI ScaI SfuI SgrAI
SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 690 GACGTC GACGT^C
AhdI 874 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2984 GTGCAC G^TGCAC
ApaLI 2984 GTGCAC G^TGCAC
AseI 3920 ATTAAT AT^TAAT
AsnI 3920 ATTAAT AT^TAAT
AspEI 874 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5303 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2641 CCTAGG C^CTAGG
BbrPI 4541 CACGTG CAC^GTG
BlnI 2641 CCTAGG C^CTAGG
BsaAI 4541 CACGTG [CT]AC^GT[GA]
BsmI 3940 GCATTC GAATGC(1/-1)
BstEII 2735 GGTAACC G^GT[ACGT]ACC
BstPI 2735 GGTAACC G^GT[ACGT]ACC
Eam1105I 874 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2735 GGTAACC G^GT[ACGT]ACC
EcoRI 5105 GAATTC G^AATTC
HindIII 1944 AAGCTT A^AGCTT
KspI 417 CCGCGG CCGC^GG
NheI 200 GCTAGC G^CTAGC
PflFI 5303 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4541 CACGTG CAC^GTG
PmlI 4541 CACGTG CAC^GTG
PshBI 3920 ATTAAT AT^TAAT
SacI 504 GAGCTC GAGCT^C
SacII 417 CCGCGG CCGC^GG
SexAI 1362 ACCTGGT A^CC[AT]GGT
Tth111I 5303 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3481 GTCTAC GT^[AC][GT]AC
3543 GTAGAC
AflIII 3547 ACACGT A^C[GA][CT]GT
4542 ACGTGT
ApaI 421 GGGCCC GGGCC^C
1723 GGGCCC
BbeI 4318 GGCGCC GGCGC^C
5184 GGCGCC
BbsI 182 GAAGAC GAAGAC(2/6)
2033 GAAGAC
BbvCI 3275 GCTGAGG CCTCAGC(-5/-2)
5076 GCTGAGG
BglII 2323 AGATCT A^GATCT
3225 AGATCT
BpuAI 182 GAAGAC GAAGAC(2/6)
2033 GAAGAC
BsmBI 5141 CGTCTC CGTCTC(1/5)
4360 GAGACG
Bsp1407I 2936 TGTACA T^GTACA
3173 TGTACA
BspHI 508 TCATGA T^CATGA
4408 TCATGA
BsrBI 2388 GAGCGG CCGCTC(-3/-3)
4121 GAGCGG
BsrDI 830 CATTGC GCAATG(2/0)
5423 GCAATG
BsrGI 2936 TGTACA T^GTACA
3173 TGTACA
BstAPI 1850 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1898 GCAGTTCATGC
DrdI 185 GACCTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3331 GACAACTCCGTC
HgaI 960 GACGC GACGC(5/10)
4960 GCGTC
HpaI 1367 GTTAAC GTT^AAC
3506 GTTAAC
KasI 4318 GGCGCC G^GCGCC
5184 GGCGCC
NarI 4318 GGCGCC GG^CGCC
5184 GGCGCC
NcoI 1583 CCATGG C^CATGG
3132 CCATGG
PshAI 23 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3000 GACTCTGGTC
RcaI 508 TCATGA T^CATGA
4408 TCATGA
SbfI 1055 CCTGCAGG CCTGCA^GG
1235 CCTGCAGG
SfiI 1079 GGCCCCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1724 GGCCCGAGGGGCC
SfoI 4318 GGCGCC GGC^GCC
5184 GGCGCC
SmaI 4266 CCCGGG CCC^GGG
5236 CCCGGG
SphI 4952 GCATGC GCATG^C
4956 GCATGC
Sse8387I 1055 CCTGCAGG CCTGCA^GG
1235 CCTGCAGG
SspBI 2936 TGTACA T^GTACA
3173 TGTACA
SspI 3851 AATATT AAT^ATT
3866 AATATT
StuI 4754 AGGCCT AGG^CCT
4931 AGGCCT
XbaI 3207 TCTAGA T^CTAGA
4565 TCTAGA
XmaCI 4266 CCCGGG C^CCGGG
5236 CCCGGG
XmaI 4266 CCCGGG C^CCGGG
5236 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbvI BciVI BclI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI