##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AcyI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AspI
AviII BbeI BbsI BcgI BpuAI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BspDI BsrFI BssHII BssSI
BstBI BstEII BstPI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
FseI FspI Hin1I HindIII HpaI
KasI KspI MluI NaeI NarI
NgoMIV NotI NruI NspV PacI
PflFI PinAI PmeI PpuMI PshAI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 114 GGTACC G^GTACC
AccII 723 CGCG CG^CG
AccIII 20 TCCGGA T^CCGGA
AclI 148 AACGTT AA^CGTT
AflIII 4036 ACATGT A^C[GA][CT]GT
AocI 4212 CCTAAGG CC^T[ACGT]AGG
ApaI 319 GGGCCC GGGCC^C
Asp718I 114 GGTACC G^GTACC
AvrII 4082 CCTAGG C^CTAGG
BalI 642 TGGCCA TGG^CCA
BamHI 3915 GGATCC G^GATCC
BanI 114 GGTACC G^G[CT][GA]CC
BbrPI 3544 CACGTG CAC^GTG
BciVI 5304 GGATAC GTATCC(6/5)
BglI 3558 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4082 CCTAGG C^CTAGG
BlpI 1081 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1081 GCTGAGC GC^T[ACGT]AGC
BsaBI 592 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 20 TCCGGA [AT]^CCGG[AT]
BseAI 20 TCCGGA T^CCGGA
BspEI 20 TCCGGA T^CCGGA
BspLU11I 4036 ACATGT A^CATGT
BspMI 3890 ACCTGC ACCTGC(4/8)
BsrBI 228 CCGCTC CCGCTC(-3/-3)
BstAPI 204 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 723 CGCG CG^CG
BstXI 1001 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4212 CCTAAGG CC^T[ACGT]AGG
CelII 1081 GCTGAGC GC^T[ACGT]AGC
EaeI 642 TGGCCA [CT]^GGCC[GA]
Eco81I 4212 CCTAAGG CC^T[ACGT]AGG
EcoRV 1183 GATATC GAT^ATC
HaeII 5068 GGCGCT [GA]GCGC^[CT]
KpnI 114 GGTACC GGTAC^C
MamI 592 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3808 CAATTG C^AATTG
MluNI 642 TGGCCA TGG^CCA
MroI 20 TCCGGA T^CCGGA
MscI 642 TGGCCA TGG^CCA
MunI 3808 CAATTG C^AATTG
MvnI 723 CGCG CG^CG
NcoI 572 CCATGG C^CATGG
NdeI 4349 CATATG CA^TATG
NheI 1755 GCTAGC G^CTAGC
PmaCI 3544 CACGTG CAC^GTG
PmlI 3544 CACGTG CAC^GTG
Psp1406I 148 AACGTT AA^CGTT
SacI 966 GAGCTC GAGCT^C
SapI 161 GCTCTTC GCTCTTC(1/4)
SnaBI 2365 TACGTA TAC^GTA
XbaI 2781 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 607 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2978 GAATATTTTC
BbvCI 829 GCTGAGG CCTCAGC(-5/-2)
5290 GCTGAGG
BclI 591 TGATCA T^GATCA
597 TGATCA
BglII 2232 AGATCT A^GATCT
4294 AGATCT
Bsp1407I 4522 TGTACA T^GTACA
5579 TGTACA
BsrGI 4522 TGTACA T^GTACA
5579 TGTACA
Bst1107I 4187 GTATAC GTA^TAC
4696 GTATAC
BstZ17I 4187 GTATAC GTA^TAC
4696 GTATAC
CfoI 724 GCGC GCG^C
5069 GCGC
DsaI 572 CCATGG C^C[GA][CT]GG
1089 CCGTGG
EcoRI 3775 GAATTC G^AATTC
4546 GAATTC
FauI 269 CCCGC CCCGC(4/6)
3947 CCCGC
FbaI 591 TGATCA T^GATCA
597 TGATCA
HgaI 207 GACGC GACGC(5/10)
1173 GCGTC
HhaI 724 GCGC GCG^C
5069 GCGC
HinP1I 724 GCGC G^CGC
5069 GCGC
PaeR7I 1695 CTCGAG C^TCGAG
5338 CTCGAG
PflMI 1273 CCAATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4556 CCACTTTCTGG
PstI 33 CTGCAG CTGCA^G
879 CTGCAG
PvuII 5162 CAGCTG CAG^CTG
5288 CAGCTG
SmaI 119 CCCGGG CCC^GGG
4633 CCCGGG
SspBI 4522 TGTACA T^GTACA
5579 TGTACA
StuI 838 AGGCCT AGG^CCT
2853 AGGCCT
Van91I 1273 CCAATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4556 CCACTTTCTGG
XcmI 3594 CCAAGTTTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5240 CCATTACAGCACTGG
XhoI 1695 CTCGAG C^TCGAG
5338 CTCGAG
XmaCI 119 CCCGGG C^CCGGG
4633 CCCGGG
XmaI 119 CCCGGG C^CCGGG
4633 CCCGGG
XmnI 607 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2978 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BfrI BmyI
BpmI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII