##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AclI AflII AhdI Aor51HI AscI
AspEI AviII AvrII BbeI BfrI
BglII BlnI BlpI Bpu1102I BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI HaeII HpaI KasI KspI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PmeI Psp1406I
PvuI RsrII SacII SalI SapI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 575 GGTACC G^GTACC
AccI 5216 GTCTAC GT^[AC][GT]AC
AgeI 1627 ACCGGT A^CCGGT
Alw44I 2073 GTGCAC G^TGCAC
ApaLI 2073 GTGCAC G^TGCAC
Asp700I 2705 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 575 GGTACC G^GTACC
AspI 672 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 3113 GTATCC GTATCC(6/5)
BsrFI 1627 ACCGGT [GA]^CCGG[CT]
BssSI 1560 CACGAG CACGAG(-5/-1)
Cfr10I 1627 ACCGGT [GA]^CCGG[CT]
DrdI 1337 GACGGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2568 GATATC GAT^ATC
KpnI 575 GGTACC GGTAC^C
PaeR7I 301 CTCGAG C^TCGAG
PflFI 672 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1627 ACCGGT A^CCGGT
PshAI 3814 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 3590 CCTGCAGG CCTGCA^GG
ScaI 112 AGTACT AGT^ACT
SmaI 1864 CCCGGG CCC^GGG
SphI 2064 GCATGC GCATG^C
Sse8387I 3590 CCTGCAGG CCTGCA^GG
StuI 908 AGGCCT AGG^CCT
Tth111I 672 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 301 CTCGAG C^TCGAG
XmaCI 1864 CCCGGG C^CCGGG
XmaI 1864 CCCGGG C^CCGGG
XmnI 2705 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2298 GACGTC GACGT^C
4660 GACGTC
AccII 675 CGCG CG^CG
1371 CGCG
AccIII 691 TCCGGA T^CCGGA
4019 TCCGGA
AcsI 1788 AAATTC [GA]^AATT[CT]
5013 GAATTT
AcyI 2298 GACGTC G[GA]^CG[CT]C
4660 GACGTC
AflIII 1252 ACATGT A^C[GA][CT]GT
4269 ACATGT
AlwNI 1879 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2661 CAGGCACTG
ApaI 309 GGGCCC GGGCC^C
1606 GGGCCC
ApoI 1788 AAATTC [GA]^AATT[CT]
5013 GAATTT
AseI 5087 ATTAAT AT^TAAT
5102 ATTAAT
AsnI 5087 ATTAAT AT^TAAT
5102 ATTAAT
BamHI 37 GGATCC G^GATCC
5095 GGATCC
BanI 575 GGTACC G^G[CT][GA]CC
1218 GGCACC
BbrPI 1587 CACGTG CAC^GTG
2828 CACGTG
BclI 964 TGATCA T^GATCA
1918 TGATCA
BsaBI 614 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3492 GATCAGCATC
BsaHI 2298 GACGTC G[GA]^CG[CT]C
4660 GACGTC
BseAI 691 TCCGGA T^CCGGA
4019 TCCGGA
BsgI 1647 GTGCAG GTGCAG(16/14)
2865 GTGCAG
BsmI 1618 GCATTC GAATGC(1/-1)
2841 GCATTC
BspEI 691 TCCGGA T^CCGGA
4019 TCCGGA
BspLU11I 1252 ACATGT A^CATGT
4269 ACATGT
BstAPI 2841 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5266 GCAGCGAGTGC
BstUI 675 CGCG CG^CG
1371 CGCG
FbaI 964 TGATCA T^GATCA
1918 TGATCA
HgaI 1178 GACGC GACGC(5/10)
676 GCGTC
Hin1I 2298 GACGTC G[GA]^CG[CT]C
4660 GACGTC
HincII 1747 GTCAAC GT[CT]^[GA]AC
2043 GTCAAC
HindII 1747 GTCAAC GT[CT]^[GA]AC
2043 GTCAAC
HindIII 1824 AAGCTT A^AGCTT
4289 AAGCTT
MamI 614 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3492 GATCAGCATC
MroI 691 TCCGGA T^CCGGA
4019 TCCGGA
MvnI 675 CGCG CG^CG
1371 CGCG
NcoI 442 CCATGG C^CATGG
2278 CCATGG
PflMI 1586 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2660 CCAGGCACTGG
PmaCI 1587 CACGTG CAC^GTG
2828 CACGTG
PmlI 1587 CACGTG CAC^GTG
2828 CACGTG
PshBI 5087 ATTAAT AT^TAAT
5102 ATTAAT
PstI 3591 CTGCAG CTGCA^G
4809 CTGCAG
SexAI 649 ACCTGGT A^CC[AT]GGT
3036 ACCTGGT
SspI 5084 AATATT AAT^ATT
5191 AATATT
Van91I 1586 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2660 CCAGGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AspHI AvaI AvaII BalI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII