##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AcyI AflII Aor51HI
ApaI AscI AspI AvaI AviII
AvrII BbeI BcgI BfrI BglII
BlnI BsaBI BsaHI BseAI BsiWI
BsmBI BsoBI BspEI BsrBI BssHII
Bst1107I BstZ17I CpoI Eco47III EcoNI
FseI FspI Hin1I HindIII HpaI
KasI KspI MamI MluI MroI
NarI NdeI NheI NotI NruI
PaeR7I PflFI PmeI RsrII SacII
SalI SapI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 4620 GTCTAC GT^[AC][GT]AC
AccII 4166 CGCG CG^CG
AclI 384 AACGTT AA^CGTT
AflIII 1334 ACATGT A^C[GA][CT]GT
AgeI 1578 ACCGGT A^CCGGT
AhdI 3741 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 536 ATTAAT AT^TAAT
AsnI 536 ATTAAT AT^TAAT
AspEI 3741 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3338 CACGTG CAC^GTG
BglI 3985 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3338 CACGTG [CT]AC^GT[GA]
Bsp1407I 2103 TGTACA T^GTACA
BspLU11I 1334 ACATGT A^CATGT
BsrDI 3247 GCAATG GCAATG(2/0)
BsrGI 2103 TGTACA T^GTACA
BssSI 3880 CTCGTG CACGAG(-5/-1)
BstAPI 320 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 643 TTCGAA TT^CGAA
BstUI 4166 CGCG CG^CG
BstXI 1263 CCAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2938 GACTTATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3741 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 116 GAAGAG CTCTTC(1/4)
HgaI 3851 GACGC GACGC(5/10)
HincII 3323 GTCAAC GT[CT]^[GA]AC
HindII 3323 GTCAAC GT[CT]^[GA]AC
Ksp632I 116 GAAGAG CTCTTC(1/4)
MvnI 4166 CGCG CG^CG
NaeI 1698 GCCGGC GCC^GGC
NgoMIV 1698 GCCGGC G^CCGGC
NspV 643 TTCGAA TT^CGAA
PacI 533 TTAATTAA TTAAT^TAA
PinAI 1578 ACCGGT A^CCGGT
PmaCI 3338 CACGTG CAC^GTG
PmlI 3338 CACGTG CAC^GTG
PshBI 536 ATTAAT AT^TAAT
Psp1406I 384 AACGTT AA^CGTT
ScaI 3863 AGTACT AGT^ACT
SexAI 1105 ACCAGGT A^CC[AT]GGT
SfuI 643 TTCGAA TT^CGAA
SpeI 4895 ACTAGT A^CTAGT
SspBI 2103 TGTACA T^GTACA
XbaI 5100 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 732 CCTTAGG CC^T[ACGT]AGG
2335 CCTGAGG
BamHI 1292 GGATCC G^GATCC
3566 GGATCC
BbsI 2437 GAAGAC GAAGAC(2/6)
4712 GAAGAC
BbvCI 2153 CCTCAGC CCTCAGC(-5/-2)
4544 CCTCAGC
BclI 2251 TGATCA T^GATCA
2269 TGATCA
BpuAI 2437 GAAGAC GAAGAC(2/6)
4712 GAAGAC
BspDI 2778 ATCGAT AT^CGAT
3344 ATCGAT
BspMI 1322 ACCTGC ACCTGC(4/8)
4235 ACCTGC
BstEII 2387 GGTTACC G^GT[ACGT]ACC
2722 GGTCACC
BstPI 2387 GGTTACC G^GT[ACGT]ACC
2722 GGTCACC
BstYI 1292 GGATCC [GA]^GATC[CT]
3566 GGATCC
Bsu36I 732 CCTTAGG CC^T[ACGT]AGG
2335 CCTGAGG
ClaI 2778 ATCGAT AT^CGAT
3344 ATCGAT
DraI 1723 TTTAAA TTT^AAA
4945 TTTAAA
DraIII 3237 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4305 CACGCTGTG
EagI 15 CGGCCG C^GGCCG
4325 CGGCCG
EclXI 15 CGGCCG C^GGCCG
4325 CGGCCG
Eco52I 15 CGGCCG C^GGCCG
4325 CGGCCG
Eco81I 732 CCTTAGG CC^T[ACGT]AGG
2335 CCTGAGG
EcoO65I 2387 GGTTACC G^GT[ACGT]ACC
2722 GGTCACC
EcoRV 701 GATATC GAT^ATC
1676 GATATC
EcoT22I 189 ATGCAT ATGCA^T
2926 ATGCAT
FbaI 2251 TGATCA T^GATCA
2269 TGATCA
MfeI 492 CAATTG C^AATTG
2139 CAATTG
MflI 1292 GGATCC [GA]^GATC[CT]
3566 GGATCC
MunI 492 CAATTG C^AATTG
2139 CAATTG
NsiI 189 ATGCAT ATGCA^T
2926 ATGCAT
PshAI 3464 GACTATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4152 GACCGGAGTC
PstI 706 CTGCAG CTGCA^G
1594 CTGCAG
SphI 187 GCATGC GCATG^C
2192 GCATGC
StuI 736 AGGCCT AGG^CCT
3255 AGGCCT
XhoII 1292 GGATCC [GA]^GATC[CT]
3566 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I Asp718I AspHI AvaII BalI
BanI BanII BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmnI