##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BclI BglI
BlnI BsaAI BsaHI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I FbaI FspI
Hin1I HpaI KasI KpnI MfeI
MluI MroI MunI NarI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 434 GTGCAC G^TGCAC
AocI 979 CCTAAGG CC^T[ACGT]AGG
Aor51HI 191 AGCGCT AGC^GCT
ApaLI 434 GTGCAC G^TGCAC
AspI 1314 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2836 GCATGGATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4266 AGATCT A^GATCT
BsaBI 3923 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1615 CGGCCG CG[GA][CT]^CG
BsmI 5293 GCATTC GAATGC(1/-1)
BspHI 2738 TCATGA T^CATGA
BsrBI 1539 GAGCGG CCGCTC(-3/-3)
Bsu36I 979 CCTAAGG CC^T[ACGT]AGG
DraIII 682 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1615 CGGCCG C^GGCCG
EclXI 1615 CGGCCG C^GGCCG
Eco47III 191 AGCGCT AGC^GCT
Eco52I 1615 CGGCCG C^GGCCG
Eco81I 979 CCTAAGG CC^T[ACGT]AGG
EcoRV 4553 GATATC GAT^ATC
EcoT22I 3374 ATGCAT ATGCA^T
FseI 1616 GGCCGGCC GGCCGG^CC
HaeII 191 AGCGCT [GA]GCGC^[CT]
HindIII 5065 AAGCTT A^AGCTT
KspI 1550 CCGCGG CCGC^GG
MamI 3923 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4117 CATATG CA^TATG
NheI 546 GCTAGC G^CTAGC
NsiI 3374 ATGCAT ATGCA^T
PaeR7I 4290 CTCGAG C^TCGAG
PflFI 1314 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2738 TCATGA T^CATGA
SacII 1550 CCGCGG CCGC^GG
SapI 4786 GCTCTTC GCTCTTC(1/4)
SbfI 691 CCTGCAGG CCTGCA^GG
ScaI 2374 AGTACT AGT^ACT
SmaI 946 CCCGGG CCC^GGG
Sse8387I 691 CCTGCAGG CCTGCA^GG
Tth111I 1314 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4290 CTCGAG C^TCGAG
XmaCI 946 CCCGGG C^CCGGG
XmaI 946 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1551 CGCG CG^CG
3631 CGCG
AflIII 75 ACATGT A^C[GA][CT]GT
3766 ACATGT
Asp700I 2039 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4562 GAATCCCTTC
BamHI 4375 GGATCC G^GATCC
4682 GGATCC
BsaI 1442 GGTCTC GGTCTC(1/5)
4151 GGTCTC
BsaWI 2761 ACCGGA [AT]^CCGG[AT]
4405 ACCGGA
BsmBI 1490 CGTCTC CGTCTC(1/5)
3011 CGTCTC
BspLU11I 75 ACATGT A^CATGT
3766 ACATGT
BsrFI 322 GCCGGC [GA]^CCGG[CT]
1617 GCCGGC
BstEII 1841 GGTGACC G^GT[ACGT]ACC
3993 GGTCACC
BstPI 1841 GGTGACC G^GT[ACGT]ACC
3993 GGTCACC
BstUI 1551 CGCG CG^CG
3631 CGCG
CfoI 192 GCGC GCG^C
3630 GCGC
Cfr10I 322 GCCGGC [GA]^CCGG[CT]
1617 GCCGGC
DrdI 1874 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2423 GACAGACAGGTC
EcoO65I 1841 GGTGACC G^GT[ACGT]ACC
3993 GGTCACC
HhaI 192 GCGC GCG^C
3630 GCGC
HinP1I 192 GCGC G^CGC
3630 GCGC
HincII 1353 GTCAAC GT[CT]^[GA]AC
4068 GTCAAC
HindII 1353 GTCAAC GT[CT]^[GA]AC
4068 GTCAAC
MvnI 1551 CGCG CG^CG
3631 CGCG
NaeI 322 GCCGGC GCC^GGC
1617 GCCGGC
NgoMIV 322 GCCGGC G^CCGGC
1617 GCCGGC
PflMI 1640 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3086 CCAGGATCTGG
SexAI 218 ACCAGGT A^CC[AT]GGT
900 ACCAGGT
SspI 3508 AATATT AAT^ATT
3515 AATATT
Van91I 1640 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3086 CCAGGATCTGG
XmnI 2039 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4562 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BfrI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII