##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BcgI
BglI BglII BlnI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CfoI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FauI FseI
FspI HaeII HhaI HinP1I KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 2722 GGCGTC G[GA]^CG[CT]C
AflII 4437 CTTAAG C^TTAAG
Alw44I 3757 GTGCAC G^TGCAC
ApaLI 3757 GTGCAC G^TGCAC
AseI 4880 ATTAAT AT^TAAT
AsnI 4880 ATTAAT AT^TAAT
Asp700I 2867 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 815 TGGCCA TGG^CCA
BanI 2191 GGCACC G^G[CT][GA]CC
BbsI 3415 GAAGAC GAAGAC(2/6)
BbvCI 1343 CCTCAGC CCTCAGC(-5/-2)
BfrI 4437 CTTAAG C^TTAAG
BlpI 4886 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4886 GCTTAGC GC^T[ACGT]AGC
BpuAI 3415 GAAGAC GAAGAC(2/6)
BsaAI 3970 CACGTA [CT]AC^GT[GA]
BsaHI 2722 GGCGTC G[GA]^CG[CT]C
BsgI 250 GTGCAG GTGCAG(16/14)
BspHI 3010 TCATGA T^CATGA
BspMI 3654 GCAGGT ACCTGC(4/8)
BssSI 1792 CTCGTG CACGAG(-5/-1)
Bst1107I 783 GTATAC GTA^TAC
BstZ17I 783 GTATAC GTA^TAC
CelII 4886 GCTTAGC GC^T[ACGT]AGC
DraIII 4012 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 815 TGGCCA [CT]^GGCC[GA]
EcoNI 1590 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 108 GATATC GAT^ATC
HgaI 2723 GCGTC GACGC(5/10)
Hin1I 2722 GGCGTC G[GA]^CG[CT]C
HindIII 208 AAGCTT A^AGCTT
MfeI 2818 CAATTG C^AATTG
MluNI 815 TGGCCA TGG^CCA
MscI 815 TGGCCA TGG^CCA
MspA1I 268 CAGCGG C[AC]G^C[GT]G
MunI 2818 CAATTG C^AATTG
PacI 4877 TTAATTAA TTAAT^TAA
PshBI 4880 ATTAAT AT^TAAT
RcaI 3010 TCATGA T^CATGA
XbaI 998 TCTAGA T^CTAGA
XmnI 2867 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 270 GCGG CCGC(-3/-1)
2720 GCGG
AflIII 3230 ACATGT A^C[GA][CT]GT
4700 ACATGT
AlwNI 2284 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2410 CAGGTCCTG
BamHI 2403 GGATCC G^GATCC
2498 GGATCC
BclI 1282 TGATCA T^GATCA
4335 TGATCA
BcnI 875 CCCGG CC^[GC]GG
2009 CCCGG
BsaWI 968 ACCGGA [AT]^CCGG[AT]
2757 ACCGGA
BsmAI 1818 GAGAC GTCTC(1/5)
51 GTCTC
BsmI 570 GCATTC GAATGC(1/-1)
4360 GCATTC
BspLU11I 3230 ACATGT A^CATGT
4700 ACATGT
EarI 198 GAAGAG CTCTTC(1/4)
3687 GAAGAG
FbaI 1282 TGATCA T^GATCA
4335 TGATCA
HpaI 1008 GTTAAC GTT^AAC
2292 GTTAAC
Ksp632I 198 GAAGAG CTCTTC(1/4)
3687 GAAGAG
MaeII 1012 ACGT A^CGT
3971 ACGT
NciI 875 CCCGG CC^[GC]GG
2009 CCCGG
NcoI 41 CCATGG C^CATGG
3992 CCATGG
NheI 1292 GCTAGC G^CTAGC
2426 GCTAGC
PflMI 2409 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4028 CCACTTTTTGG
PleI 4380 GACTC GAGTC(4/5)
3116 GAGTC
PshAI 1601 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3002 GACAACAGTC
PstI 3488 CTGCAG CTGCA^G
4157 CTGCAG
ScaI 1247 AGTACT AGT^ACT
4677 AGTACT
TaqI 1523 TCGA T^CGA
3073 TCGA
TthHB8I 1523 TCGA T^CGA
3073 TCGA
Van91I 2409 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4028 CCACTTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BciVI BfaI BmyI BpmI BsaBI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PpuMI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII