##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI ApoI AscI
AseI AsnI Asp718I AvrII BclI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HpaI KpnI
MfeI MroI MunI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfuI SgrAI
SnaBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AhdI 614 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3865 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 614 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3405 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2388 TGCGCA TGC^GCA
BcgI 2035 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1176 GTATCC GTATCC(6/5)
BsaBI 70 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 2845 TGTACA T^GTACA
BsrDI 1382 GCAATG GCAATG(2/0)
BsrGI 2845 TGTACA T^GTACA
BssSI 3848 CTCGTG CACGAG(-5/-1)
DrdI 1468 GACAAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1101 CGGCCG C^GGCCG
Eam1105I 614 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1101 CGGCCG C^GGCCG
Eco52I 1101 CGGCCG C^GGCCG
FspI 2388 TGCGCA TGC^GCA
KspI 11 CCGCGG CCGC^GG
MamI 70 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2849 CATATG CA^TATG
PaeR7I 1060 CTCGAG C^TCGAG
PflFI 3405 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2395 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 11 CCGCGG CCGC^GG
SapI 1877 GCTCTTC GCTCTTC(1/4)
SbfI 3634 CCTGCAGG CCTGCA^GG
SmaI 4242 CCCGGG CCC^GGG
SpeI 2896 ACTAGT A^CTAGT
SphI 1873 GCATGC GCATG^C
Sse8387I 3634 CCTGCAGG CCTGCA^GG
SspBI 2845 TGTACA T^GTACA
Tth111I 3405 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1060 CTCGAG C^TCGAG
XmaCI 4242 CCCGGG C^CCGGG
XmaI 4242 CCCGGG C^CCGGG
XmnI 3865 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 3090 GTGCAC G^TGCAC
3245 GTGCAC
AocI 1432 CCTGAGG CC^T[ACGT]AGG
4748 CCTCAGG
ApaLI 3090 GTGCAC G^TGCAC
3245 GTGCAC
BbsI 2335 GTCTTC GAAGAC(2/6)
2477 GTCTTC
BglII 543 AGATCT A^GATCT
795 AGATCT
BpuAI 2335 GTCTTC GAAGAC(2/6)
2477 GTCTTC
BsiEI 901 CGACCG CG[GA][CT]^CG
1101 CGGCCG
BsmI 871 GAATGC GAATGC(1/-1)
2343 GCATTC
Bsu36I 1432 CCTGAGG CC^T[ACGT]AGG
4748 CCTCAGG
DraI 2974 TTTAAA TTT^AAA
3143 TTTAAA
Eco81I 1432 CCTGAGG CC^T[ACGT]AGG
4748 CCTCAGG
HincII 2892 GTTGAC GT[CT]^[GA]AC
3713 GTTGAC
HindII 2892 GTTGAC GT[CT]^[GA]AC
3713 GTTGAC
HindIII 2570 AAGCTT A^AGCTT
4508 AAGCTT
MluI 100 ACGCGT A^CGCGT
850 ACGCGT
NaeI 338 GCCGGC GCC^GGC
3622 GCCGGC
NcoI 33 CCATGG C^CATGG
3876 CCATGG
NgoMIV 338 GCCGGC G^CCGGC
3622 GCCGGC
SexAI 759 ACCTGGT A^CC[AT]GGT
4687 ACCTGGT
StuI 2027 AGGCCT AGG^CCT
3547 AGGCCT
TfiI 1054 GAATC G^A[AT]TC
3426 GATTC
Tsp509I 3866 AATT ^AATT
4160 AATT
XcmI 2440 CCAGCAGCGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2697 CCATTTCATCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmlI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XhoII