##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AocI Aor51HI
ApaI AscI Asp718I AspI AvaI
AvrII BalI BanII BbeI BbrPI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII Cfr10I ClaI CpoI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FseI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1505 GTATAC GT^[AC][GT]AC
AseI 1625 ATTAAT AT^TAAT
AsnI 1625 ATTAAT AT^TAAT
AviII 1022 TGCGCA TGC^GCA
BanI 1488 GGTGCC G^G[CT][GA]CC
BbvCI 2514 CCTCAGC CCTCAGC(-5/-2)
BcgI 2728 CGACTGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2933 GGATAC GTATCC(6/5)
BclI 2033 TGATCA T^GATCA
BsaBI 1296 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 3358 GAGGAG GAGGAG(10/8)
BspHI 287 TCATGA T^CATGA
BssSI 3578 CACGAG CACGAG(-5/-1)
Bst1107I 1505 GTATAC GTA^TAC
BstXI 1408 CCAGTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1505 GTATAC GTA^TAC
CfoI 1023 GCGC GCG^C
DraIII 829 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1798 GACAGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1956 GATATC GAT^ATC
FauI 4400 CCCGC CCCGC(4/6)
FbaI 2033 TGATCA T^GATCA
FspI 1022 TGCGCA TGC^GCA
HhaI 1023 GCGC GCG^C
HinP1I 1023 GCGC G^CGC
MamI 1296 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2383 CATATG CA^TATG
PflMI 1495 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1625 ATTAAT AT^TAAT
PstI 1253 CTGCAG CTGCA^G
RcaI 287 TCATGA T^CATGA
SapI 3364 GAAGAGC GCTCTTC(1/4)
SexAI 5179 ACCAGGT A^CC[AT]GGT
SmlI 411 CTTGAG C^T[CT][GA]AG
SphI 248 GCATGC GCATG^C
StuI 539 AGGCCT AGG^CCT
Van91I 1495 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2394 TCTAGA T^CTAGA
XcmI 2920 CCAAACCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 1231 CCGC CCGC(-3/-1)
4401 CCGC
AhdI 3999 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACAAACAGTC
Alw44I 1226 GTGCAC G^TGCAC
3862 GTGCAC
AlwNI 1059 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1348 CAGTTACTG
ApaLI 1226 GTGCAC G^TGCAC
3862 GTGCAC
Asp700I 891 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1872 GAAAATGTTC
AspEI 3999 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACAAACAGTC
BamHI 1751 GGATCC G^GATCC
4363 GGATCC
BsaI 4991 GAGACC GGTCTC(1/5)
4219 GGTCTC
BsmFI 1055 GGGAC GGGAC(10/14)
2985 GGGAC
BspMI 1481 ACCTGC ACCTGC(4/8)
2309 ACCTGC
DraII 2129 AGGACCT [GA]G^G[ACGT]CC[CT]
2486 AGGTCCT
Eam1105I 3999 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACAAACAGTC
EcoO109I 2129 AGGACCT [GA]G^G[ACGT]CC[CT]
2486 AGGTCCT
HaeIII 540 GGCC GG^CC
4118 GGCC
MfeI 2068 CAATTG C^AATTG
5819 CAATTG
MspA1I 4264 CAGCTG C[AC]G^C[GT]G
4547 CAGCTG
MunI 2068 CAATTG C^AATTG
5819 CAATTG
NheI 848 GCTAGC G^CTAGC
1818 GCTAGC
PpuMI 2129 AGGACCT [GA]G^G[AT]CC[CT]
2486 AGGTCCT
PvuII 4264 CAGCTG CAG^CTG
4547 CAGCTG
ScaI 3190 AGTACT AGT^ACT
3649 AGTACT
XmnI 891 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1872 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII