##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
Asp718I AviII BcgI BciVI BclI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
DraI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RcaI SalI ScaI SexAI
SfuI SgrAI SnaBI SphI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2785 GACGTC GACGT^C
AccI 772 GTAGAC GT^[AC][GT]AC
AcsI 206 AAATTC [GA]^AATT[CT]
AflII 1842 CTTAAG C^TTAAG
AhdI 1986 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1727 AGCGCT AGC^GCT
ApoI 206 AAATTC [GA]^AATT[CT]
AseI 2293 ATTAAT AT^TAAT
AsnI 2293 ATTAAT AT^TAAT
AspEI 1986 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3067 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2832 CCTAGG C^CTAGG
BamHI 1004 GGATCC G^GATCC
BbrPI 363 CACGTG CAC^GTG
BfrI 1842 CTTAAG C^TTAAG
BglII 1456 AGATCT A^GATCT
BlnI 2832 CCTAGG C^CTAGG
BsaAI 363 CACGTG [CT]AC^GT[GA]
BsaBI 999 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1997 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3239 TGTACA T^GTACA
BspLU11I 1745 ACATGT A^CATGT
BsrGI 3239 TGTACA T^GTACA
BssSI 3037 CTCGTG CACGAG(-5/-1)
CpoI 1765 CGGACCG CG^G[AT]CCG
DrdI 2868 GACTGACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1986 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1727 AGCGCT AGC^GCT
HindIII 2093 AAGCTT A^AGCTT
KspI 2352 CCGCGG CCGC^GG
MamI 999 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 1 TCGCGA TCG^CGA
PflFI 3067 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 363 CACGTG CAC^GTG
PmlI 363 CACGTG CAC^GTG
PshBI 2293 ATTAAT AT^TAAT
RsrII 1765 CGGACCG CG^G[AT]CCG
SacII 2352 CCGCGG CCGC^GG
SbfI 1917 CCTGCAGG CCTGCA^GG
SmaI 4207 CCCGGG CCC^GGG
SpeI 13 ACTAGT A^CTAGT
Sse8387I 1917 CCTGCAGG CCTGCA^GG
SspBI 3239 TGTACA T^GTACA
StuI 1700 AGGCCT AGG^CCT
TaqI 1883 TCGA T^CGA
Tth111I 3067 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1883 TCGA T^CGA
XmaCI 4207 CCCGGG C^CCGGG
XmaI 4207 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 1745 ACATGT A^C[GA][CT]GT
2873 ACACGT
Alw44I 536 GTGCAC G^TGCAC
3040 GTGCAC
AocI 870 CCTGAGG CC^T[ACGT]AGG
3554 CCTGAGG
ApaLI 536 GTGCAC G^TGCAC
3040 GTGCAC
Asp700I 2503 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2985 GAAGGCTTTC
BsmBI 191 CGTCTC CGTCTC(1/5)
2787 CGTCTC
BsrBI 3782 CCGCTC CCGCTC(-3/-3)
4106 GAGCGG
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
3554 CCTGAGG
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
3554 CCTGAGG
MslI 57 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1735 CACTGGAGTG
SacI 1899 GAGCTC GAGCT^C
2844 GAGCTC
SapI 2674 GAAGAGC GCTCTTC(1/4)
3793 GAAGAGC
SfiI 877 GGCCATTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3583 GGCCCTGATGGCC
TfiI 2727 GATTC G^A[AT]TC
3199 GAATC
XmnI 2503 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2985 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoII