##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI Alw44I Aor51HI ApaLI AscI
Asp700I Asp718I AspI AviII AvrII
BbeI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BssSI BstBI BstEII BstPI
BstUI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HaeII Hin1I KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PmeI Psp1406I PvuI RsrII
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 2759 CTTAAG C^TTAAG
AflIII 3155 ACATGT A^C[GA][CT]GT
AhdI 446 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3552 ATTAAT AT^TAAT
AsnI 3552 ATTAAT AT^TAAT
AspEI 446 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 172 CCCGAG C^[CT]CG[GA]G
BamHI 2046 GGATCC G^GATCC
BanI 1844 GGCACC G^G[CT][GA]CC
BbrPI 3380 CACGTG CAC^GTG
BbsI 659 GAAGAC GAAGAC(2/6)
BbvCI 1977 CCTCAGC CCTCAGC(-5/-2)
BcgI 1337 CGATGATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2393 TGATCA T^GATCA
BcnI 2438 CCGGG CC^[GC]GG
BfrI 2759 CTTAAG C^TTAAG
BglI 274 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 615 AGATCT A^GATCT
BpuAI 659 GAAGAC GAAGAC(2/6)
BsaAI 3380 CACGTG [CT]AC^GT[GA]
BsmBI 424 CGTCTC CGTCTC(1/5)
BsmI 2335 GCATTC GAATGC(1/-1)
BsoBI 172 CCCGAG C^[CT]CG[GA]G
BspLU11I 3155 ACATGT A^CATGT
BstAPI 948 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 560 GCGC GCG^C
DraIII 3415 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 446 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 163 GAATTC G^AATTC
FbaI 2393 TGATCA T^GATCA
HhaI 560 GCGC GCG^C
HinP1I 560 GCGC G^CGC
HindIII 4583 AAGCTT A^AGCTT
HpaI 3781 GTTAAC GTT^AAC
MfeI 4983 CAATTG C^AATTG
MunI 4983 CAATTG C^AATTG
NciI 2438 CCGGG CC^[GC]GG
NcoI 3680 CCATGG C^CATGG
NheI 4329 GCTAGC G^CTAGC
PmaCI 3380 CACGTG CAC^GTG
PmlI 3380 CACGTG CAC^GTG
PshAI 1619 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3552 ATTAAT AT^TAAT
SacI 1168 GAGCTC GAGCT^C
SalI 2862 GTCGAC G^TCGAC
SexAI 4215 ACCAGGT A^CC[AT]GGT
SspI 4837 AATATT AAT^ATT
StuI 85 AGGCCT AGG^CCT
XbaI 3026 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AgeI 937 ACCGGT A^CCGGT
2650 ACCGGT
AlwNI 949 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
4509 CAGGCTCTG
ApaI 135 GGGCCC GGGCC^C
2952 GGGCCC
Bst1107I 1602 GTATAC GTA^TAC
4383 GTATAC
BstZ17I 1602 GTATAC GTA^TAC
4383 GTATAC
DraI 3267 TTTAAA TTT^AAA
4927 TTTAAA
DsaI 905 CCACGG C^C[GA][CT]GG
3680 CCATGG
EcoT22I 3307 ATGCAT ATGCA^T
3907 ATGCAT
HgaI 1347 GCGTC GACGC(5/10)
2696 GCGTC
NsiI 3307 ATGCAT ATGCA^T
3907 ATGCAT
PinAI 937 ACCGGT A^CCGGT
2650 ACCGGT
XcmI 112 CCAGGTGGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2625 CCATGTGTTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaII
BalI BanII BbvI BciVI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII