##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I ApaLI Asp700I
Asp718I AviII AvrII BamHI BbsI
BciVI BclI BfrI BlnI BpuAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MfeI MluI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SalI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 4589 AAATTT [GA]^AATT[CT]
Aor51HI 2021 AGCGCT AGC^GCT
ApoI 4589 AAATTT [GA]^AATT[CT]
AscI 3163 GGCGCGCC GG^CGCGCC
AseI 3901 ATTAAT AT^TAAT
AsnI 3901 ATTAAT AT^TAAT
BglII 290 AGATCT A^GATCT
BlpI 2364 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2364 GCTCAGC GC^T[ACGT]AGC
BsaI 4525 GGTCTC GGTCTC(1/5)
BsmBI 438 CGTCTC CGTCTC(1/5)
CelII 2364 GCTCAGC GC^T[ACGT]AGC
EarI 2172 GAAGAG CTCTTC(1/4)
Eco47III 2021 AGCGCT AGC^GCT
HincII 1957 GTCAAC GT[CT]^[GA]AC
HindII 1957 GTCAAC GT[CT]^[GA]AC
Ksp632I 2172 GAAGAG CTCTTC(1/4)
NheI 4117 GCTAGC G^CTAGC
PshAI 3843 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3901 ATTAAT AT^TAAT
SapI 2172 GAAGAGC GCTCTTC(1/4)
SspI 3986 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 1096 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4027 GACACGAAGTC
AlwI 3339 GATCC GGATC(4/5)
4547 GATCC
AspEI 1096 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4027 GACACGAAGTC
AspI 2595 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2605 GACACGGTC
BbrPI 3178 CACGTG CAC^GTG
4312 CACGTG
BsaBI 918 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1053 GATGGCCATC
BsrDI 2798 CATTGC GCAATG(2/0)
158 GCAATG
BssHII 2952 GCGCGC G^CGCGC
3164 GCGCGC
BstYI 290 AGATCT [GA]^GATC[CT]
3338 AGATCC
DraI 3974 TTTAAA TTT^AAA
4592 TTTAAA
DrdI 1547 GACCTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1948 GACAAGAACGTC
Eam1105I 1096 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4027 GACACGAAGTC
MamI 918 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1053 GATGGCCATC
MflI 290 AGATCT [GA]^GATC[CT]
3338 AGATCC
MslI 112 CATCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4411 CACCCCAGTG
NspI 3419 GCATGT [GA]CATG^[CT]
4326 GCATGT
PaeR7I 103 CTCGAG C^TCGAG
4208 CTCGAG
PflFI 2595 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2605 GACACGGTC
PmaCI 3178 CACGTG CAC^GTG
4312 CACGTG
PmlI 3178 CACGTG CAC^GTG
4312 CACGTG
SacI 585 GAGCTC GAGCT^C
1135 GAGCTC
SbfI 18 CCTGCAGG CCTGCA^GG
909 CCTGCAGG
ScaI 839 AGTACT AGT^ACT
4515 AGTACT
Sse8387I 18 CCTGCAGG CCTGCA^GG
909 CCTGCAGG
TfiI 1818 GAATC G^A[AT]TC
3995 GATTC
Tth111I 2595 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2605 GACACGGTC
XhoI 103 CTCGAG C^TCGAG
4208 CTCGAG
XhoII 290 AGATCT [GA]^GATC[CT]
3338 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MroI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI