##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AspI AvrII BamHI BbeI
BbvCI BcgI BlnI BsaWI BseAI
BsiWI BspDI BspEI BspHI BsrFI
Bst1107I BstEII BstPI BstZ17I Cfr10I
ClaI DraIII Eco47III EcoO65I FseI
HaeII KasI KpnI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI PacI PflFI PinAI
PmeI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 65 GGCGTC G[GA]^CG[CT]C
AhdI 1876 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 59 GGGCCC GGGCC^C
AseI 3540 ATTAAT AT^TAAT
AsnI 3540 ATTAAT AT^TAAT
AspEI 1876 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 5295 TGGCCA TGG^CCA
BbrPI 2921 CACGTG CAC^GTG
BciVI 5359 GTATCC GTATCC(6/5)
BclI 2007 TGATCA T^GATCA
BglI 3111 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2288 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2288 GCTAAGC GC^T[ACGT]AGC
BsaAI 2921 CACGTG [CT]AC^GT[GA]
BsaBI 3757 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 65 GGCGTC G[GA]^CG[CT]C
BsiEI 2343 CGGCCG CG[GA][CT]^CG
BsmBI 173 CGTCTC CGTCTC(1/5)
BsmI 766 GCATTC GAATGC(1/-1)
BstAPI 1746 GCAAACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5391 TTCGAA TT^CGAA
BstXI 4880 CCATACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2288 GCTAAGC GC^T[ACGT]AGC
CpoI 1566 CGGACCG CG^G[AT]CCG
DrdI 2955 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2343 CGGCCG C^GGCCG
Eam1105I 1876 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2343 CGGCCG C^GGCCG
Eco52I 2343 CGGCCG C^GGCCG
EcoRI 5197 GAATTC G^AATTC
EcoRV 4634 GATATC GAT^ATC
FbaI 2007 TGATCA T^GATCA
Hin1I 65 GGCGTC G[GA]^CG[CT]C
HindIII 2934 AAGCTT A^AGCTT
MamI 3757 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3298 CAATTG C^AATTG
MluNI 5295 TGGCCA TGG^CCA
MscI 5295 TGGCCA TGG^CCA
MunI 3298 CAATTG C^AATTG
NspV 5391 TTCGAA TT^CGAA
PmaCI 2921 CACGTG CAC^GTG
PmlI 2921 CACGTG CAC^GTG
PshBI 3540 ATTAAT AT^TAAT
RsrII 1566 CGGACCG CG^G[AT]CCG
SacI 228 GAGCTC GAGCT^C
SapI 388 GAAGAGC GCTCTTC(1/4)
SfuI 5391 TTCGAA TT^CGAA
XbaI 904 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 1757 CTTAAG C^TTAAG
3715 CTTAAG
AocI 1321 CCTCAGG CC^T[ACGT]AGG
2365 CCTCAGG
AviII 1383 TGCGCA TGC^GCA
2445 TGCGCA
BcnI 62 CCCGG CC^[GC]GG
624 CCCGG
BfrI 1757 CTTAAG C^TTAAG
3715 CTTAAG
BglII 1761 AGATCT A^GATCT
2090 AGATCT
BspMI 2429 ACCTGC ACCTGC(4/8)
1778 GCAGGT
BsrBI 49 GAGCGG CCGCTC(-3/-3)
2324 GAGCGG
BssHII 30 GCGCGC G^CGCGC
32 GCGCGC
BssSI 2321 CACGAG CACGAG(-5/-1)
2399 CACGAG
Bsu36I 1321 CCTCAGG CC^T[ACGT]AGG
2365 CCTCAGG
Eco81I 1321 CCTCAGG CC^T[ACGT]AGG
2365 CCTCAGG
EcoNI 160 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3322 CCTATGGGAGG
EcoT22I 2174 ATGCAT ATGCA^T
5129 ATGCAT
FauI 1408 CCCGC CCCGC(4/6)
2801 GCGGG
FspI 1383 TGCGCA TGC^GCA
2445 TGCGCA
NciI 62 CCCGG CC^[GC]GG
624 CCCGG
NdeI 4209 CATATG CA^TATG
5154 CATATG
NsiI 2174 ATGCAT ATGCA^T
5129 ATGCAT
PshAI 1835 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2702 GACCTTAGTC
PstI 2359 CTGCAG CTGCA^G
4361 CTGCAG
SexAI 868 ACCAGGT A^CC[AT]GGT
2481 ACCAGGT
SphI 2227 GCATGC GCATG^C
2442 GCATGC
StuI 1295 AGGCCT AGG^CCT
3118 AGGCCT
SwaI 1738 ATTTAAAT ATTT^AAAT
3891 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoI XhoII
XmnI