##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AlwNI Aor51HI
AscI Asp718I AspEI AspI AvaI
AviII BamHI BanI BbeI BbrPI
BglI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3419 GTCTAC GT^[AC][GT]AC
AflII 396 CTTAAG C^TTAAG
Alw44I 2475 GTGCAC G^TGCAC
AocI 1244 CCTGAGG CC^T[ACGT]AGG
ApaI 3734 GGGCCC GGGCC^C
ApaLI 2475 GTGCAC G^TGCAC
Asp700I 637 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 5177 TGGCCA TGG^CCA
BcgI 3776 CGAAGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 157 GTATCC GTATCC(6/5)
BfrI 396 CTTAAG C^TTAAG
BglII 281 AGATCT A^GATCT
BsgI 4668 CTGCAC GTGCAG(16/14)
BsmFI 2171 GTCCC GGGAC(10/14)
BspMI 2414 ACCTGC ACCTGC(4/8)
BsrDI 3711 GCAATG GCAATG(2/0)
Bsu36I 1244 CCTGAGG CC^T[ACGT]AGG
DrdI 1427 GACTGTTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1244 CCTGAGG CC^T[ACGT]AGG
EcoNI 1071 CCTAATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HpaI 792 GTTAAC GTT^AAC
MfeI 670 CAATTG C^AATTG
MluNI 5177 TGGCCA TGG^CCA
MscI 5177 TGGCCA TGG^CCA
MunI 670 CAATTG C^AATTG
NheI 1741 GCTAGC G^CTAGC
PflMI 5235 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Van91I 5235 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4030 TCTAGA T^CTAGA
XcmI 3918 CCAAGGATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 637 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AvaII 3557 GGTCC G^G[AT]CC
4957 GGTCC
AvrII 118 CCTAGG C^CTAGG
4006 CCTAGG
BanII 3734 GGGCCC G[GA]GC[CT]^C
5066 GGGCTC
BbvCI 5086 CCTCAGC CCTCAGC(-5/-2)
5225 CCTCAGC
BlnI 118 CCTAGG C^CTAGG
4006 CCTAGG
BsaBI 3485 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4272 GATTCCGATC
BsrBI 2367 CCGCTC CCGCTC(-3/-3)
3304 GAGCGG
BssSI 480 CTCGTG CACGAG(-5/-1)
5080 CTCGTG
BstBI 2263 TTCGAA TT^CGAA
2284 TTCGAA
BstYI 281 AGATCT [GA]^GATC[CT]
4033 AGATCC
DraII 3733 GGGGCCC [GA]G^G[ACGT]CC[CT]
3734 GGGCCCC
DraIII 248 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3321 CACTGAGTG
DsaI 210 CCATGG C^C[GA][CT]GG
2611 CCATGG
EaeI 151 CGGCCA [CT]^GGCC[GA]
5177 TGGCCA
EcoO109I 3733 GGGGCCC [GA]G^G[ACGT]CC[CT]
3734 GGGCCCC
EcoRI 1188 GAATTC G^AATTC
1269 GAATTC
FauI 2366 CCCGC CCCGC(4/6)
3738 CCCGC
MaeII 274 ACGT A^CGT
1086 ACGT
MamI 3485 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4272 GATTCCGATC
MflI 281 AGATCT [GA]^GATC[CT]
4033 AGATCC
MspA1I 3998 CAGCTG C[AC]G^C[GT]G
4116 CAGCTG
NcoI 210 CCATGG C^CATGG
2611 CCATGG
NspV 2263 TTCGAA TT^CGAA
2284 TTCGAA
PvuII 3998 CAGCTG CAG^CTG
4116 CAGCTG
ScaI 435 AGTACT AGT^ACT
2742 AGTACT
SexAI 1110 ACCTGGT A^CC[AT]GGT
3690 ACCTGGT
SfuI 2263 TTCGAA TT^CGAA
2284 TTCGAA
SpeI 341 ACTAGT A^CTAGT
4554 ACTAGT
SphI 3238 GCATGC GCATG^C
5116 GCATGC
XhoII 281 AGATCT [GA]^GATC[CT]
4033 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BspHI BspLU11I
BsrGI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI PstI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I