##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

Acc65I      AccIII      AgeI        AhdI        Aor51HI     
ApaI        AscI        Asp700I     Asp718I     AspEI       
AviII       BbeI        BcgI        BglI        BglII       
BlpI        Bpu1102I    BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BsrBI       BsrFI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     FauI        
FseI        FspI        HaeII       HgaI        HindIII     
KasI        KpnI        KspI        MamI        MluI        
MroI        NaeI        NarI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PpuMI       PshAI       PvuI        RsrII       SacII       
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        1612  GACGTC               GACGT^C
AccII         940  CGCG                 CG^CG
AclI         3913  AACGTT               AA^CGTT
AcyI         1612  GACGTC               G[GA]^CG[CT]C
AflII        4980  CTTAAG               C^TTAAG
Alw44I       1520  GTGCAC               G^TGCAC
ApaLI        1520  GTGCAC               G^TGCAC
AseI         4308  ATTAAT               AT^TAAT
AsnI         4308  ATTAAT               AT^TAAT
AvrII        3373  CCTAGG               C^CTAGG
BamHI        5882  GGATCC               G^GATCC
BbrPI        1501  CACGTG               CAC^GTG
BfrI         4980  CTTAAG               C^TTAAG
BlnI         3373  CCTAGG               C^CTAGG
BsaAI        1501  CACGTG               [CT]AC^GT[GA]
BsaHI        1612  GACGTC               G[GA]^CG[CT]C
Bsp1407I     4513  TGTACA               T^GTACA
BspMI        1441  GCAGGT               ACCTGC(4/8)
BsrGI        4513  TGTACA               T^GTACA
BssSI        1414  CTCGTG               CACGAG(-5/-1)
BstUI         940  CGCG                 CG^CG
DraIII       2163  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        3362  GAATTC               G^AATTC
Hin1I        1612  GACGTC               G[GA]^CG[CT]C
HpaI         5639  GTTAAC               GTT^AAC
MfeI         1154  CAATTG               C^AATTG
MunI         1154  CAATTG               C^AATTG
MvnI          940  CGCG                 CG^CG
NdeI         2369  CATATG               CA^TATG
NheI         3420  GCTAGC               G^CTAGC
PmaCI        1501  CACGTG               CAC^GTG
PmeI         5628  GTTTAAAC             GTTT^AAAC
PmlI         1501  CACGTG               CAC^GTG
PshBI        4308  ATTAAT               AT^TAAT
Psp1406I     3913  AACGTT               AA^CGTT
SalI         2155  GTCGAC               G^TCGAC
SphI         2103  GCATGC               GCATG^C
SspBI        4513  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflIII       3002  ACATGT               A^C[GA][CT]GT
             5332  ACATGT              
AlwNI         268  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1403  CAGTGGCTG           
AvaI         4025  CCCGGG               C^[CT]CG[GA]G
             4223  CCCGGG              
AvaII         308  GGACC                G^G[AT]CC
             1311  GGACC               
BalI          869  TGGCCA               TGG^CCA
             1890  TGGCCA              
BanI           11  GGTGCC               G^G[CT][GA]CC
             1578  GGTGCC              
BclI         1752  TGATCA               T^GATCA
             4958  TGATCA              
BsmI          227  GAATGC               GAATGC(1/-1)
             1030  GAATGC              
BsoBI        4025  CCCGGG               C^[CT]CG[GA]G
             4223  CCCGGG              
BspLU11I     3002  ACATGT               A^CATGT
             5332  ACATGT              
BsrDI        4260  CATTGC               GCAATG(2/0)
              106  GCAATG              
BstAPI       1402  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3518  GCAGGAAATGC         
EaeI          869  TGGCCA               [CT]^GGCC[GA]
             1890  TGGCCA              
EcoNI         615  CCTCAATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5617  CCTCAAATAGG         
EcoRV        1904  GATATC               GAT^ATC
             4872  GATATC              
FbaI         1752  TGATCA               T^GATCA
             4958  TGATCA              
MluNI         869  TGGCCA               TGG^CCA
             1890  TGGCCA              
MscI          869  TGGCCA               TGG^CCA
             1890  TGGCCA              
MspA1I       1944  CAGCTG               C[AC]G^C[GT]G
             3118  CAGCTG              
PflMI        1975  CCAGAGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5254  CCACATGCTGG         
PvuII        1944  CAGCTG               CAG^CTG
             3118  CAGCTG              
SacI          187  GAGCTC               GAGCT^C
             2553  GAGCTC              
SmaI         4025  CCCGGG               CCC^GGG
             4223  CCCGGG              
StuI         2097  AGGCCT               AGG^CCT
             4583  AGGCCT              
TaqI         1616  TCGA                 T^CGA
             2156  TCGA                
TthHB8I      1616  TCGA                 T^CGA
             2156  TCGA                
Van91I       1975  CCAGAGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5254  CCACATGCTGG         
XbaI          954  TCTAGA               T^CTAGA
             2403  TCTAGA              
XmaCI        4025  CCCGGG               C^CCGGG
             4223  CCCGGG              
XmaI         4025  CCCGGG               C^CCGGG
             4223  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApoI        AspHI       AspI        
BanII       BbsI        BbvCI       BbvI        BciVI       
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspHI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     EcoT22I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflFI       
PleI        PstI        RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
XcmI        XhoII