##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AviII BcgI BciVI
BclI BfrI BsaBI BsiEI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco52I EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI MamI MfeI MluI
MunI NdeI NheI NotI NruI
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SgrAI SnaBI SpeI
SphI SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2043 GGTACC G^GTACC
AccI 1198 GTCTAC GT^[AC][GT]AC
AccIII 4326 TCCGGA T^CCGGA
AcsI 2453 GAATTC [GA]^AATT[CT]
AhdI 3972 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1673 CCTGAGG CC^T[ACGT]AGG
ApoI 2453 GAATTC [GA]^AATT[CT]
Asp700I 2431 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2043 GGTACC G^GTACC
AspEI 3972 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 27 GGCGCC GGCGC^C
BbrPI 2840 CACGTG CAC^GTG
BsaAI 2840 CACGTG [CT]AC^GT[GA]
BseAI 4326 TCCGGA T^CCGGA
BsmBI 1518 GAGACG CGTCTC(1/5)
BsmI 1643 GCATTC GAATGC(1/-1)
BspEI 4326 TCCGGA T^CCGGA
BsrDI 4343 CATTGC GCAATG(2/0)
BssHII 292 GCGCGC G^CGCGC
BstBI 1050 TTCGAA TT^CGAA
Bsu36I 1673 CCTGAGG CC^T[ACGT]AGG
Eam1105I 3972 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1673 CCTGAGG CC^T[ACGT]AGG
EcoRI 2453 GAATTC G^AATTC
KasI 27 GGCGCC G^GCGCC
KpnI 2043 GGTACC GGTAC^C
KspI 364 CCGCGG CCGC^GG
MroI 4326 TCCGGA T^CCGGA
NarI 27 GGCGCC GG^CGCC
NspI 707 ACATGC [GA]CATG^[CT]
NspV 1050 TTCGAA TT^CGAA
PmaCI 2840 CACGTG CAC^GTG
PmlI 2840 CACGTG CAC^GTG
PshAI 991 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 364 CCGCGG CCGC^GG
SbfI 2956 CCTGCAGG CCTGCA^GG
SfoI 27 GGCGCC GGC^GCC
SfuI 1050 TTCGAA TT^CGAA
Sse8387I 2956 CCTGCAGG CCTGCA^GG
SspI 4687 AATATT AAT^ATT
XmnI 2431 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2565 ACGTGT A^C[GA][CT]GT
2841 ACGTGT
Alw44I 2820 GTGCAC G^TGCAC
2941 GTGCAC
Aor51HI 314 AGCGCT AGC^GCT
4079 AGCGCT
ApaLI 2820 GTGCAC G^TGCAC
2941 GTGCAC
AvrII 622 CCTAGG C^CTAGG
3115 CCTAGG
BglII 1337 AGATCT A^GATCT
2499 AGATCT
BlnI 622 CCTAGG C^CTAGG
3115 CCTAGG
BsaWI 2214 ACCGGA [AT]^CCGG[AT]
4326 TCCGGA
BssSI 3476 CACGAG CACGAG(-5/-1)
3677 CACGAG
DraIII 1223 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2651 CACACGGTG
Eco47III 314 AGCGCT AGC^GCT
4079 AGCGCT
EcoT22I 1641 ATGCAT ATGCA^T
4310 ATGCAT
NaeI 2891 GCCGGC GCC^GGC
2922 GCCGGC
NcoI 3934 CCATGG C^CATGG
4174 CCATGG
NgoMIV 2891 GCCGGC G^CCGGC
2922 GCCGGC
NsiI 1641 ATGCAT ATGCA^T
4310 ATGCAT
SapI 376 GAAGAGC GCTCTTC(1/4)
526 GCTCTTC
SexAI 938 ACCTGGT A^CC[AT]GGT
1224 ACCTGGT
Tsp509I 2454 AATT ^AATT
4681 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI