##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
BbeI BbrPI BcgI BfrI BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BssHII Bst1107I BstZ17I ClaI
CpoI DraIII EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FseI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SfiI SfoI SmaI SnaBI SpeI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccII 24 CGCG CG^CG
AcyI 2331 GGCGTC G[GA]^CG[CT]C
AgeI 1921 ACCGGT A^CCGGT
Aor51HI 730 AGCGCT AGC^GCT
BamHI 1708 GGATCC G^GATCC
BglI 3036 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 298 AGATCT A^GATCT
BsaHI 2331 GGCGTC G[GA]^CG[CT]C
Bsp1407I 2509 TGTACA T^GTACA
BsrGI 2509 TGTACA T^GTACA
BssSI 230 CACGAG CACGAG(-5/-1)
BstAPI 1918 GCAACCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 24 CGCG CG^CG
DraI 786 TTTAAA TTT^AAA
DrdI 2996 GACGAGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 730 AGCGCT AGC^GCT
Hin1I 2331 GGCGTC G[GA]^CG[CT]C
HindIII 3404 AAGCTT A^AGCTT
MvnI 24 CGCG CG^CG
NdeI 2513 CATATG CA^TATG
PinAI 1921 ACCGGT A^CCGGT
PshAI 182 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 3643 CCTGCAGG CCTGCA^GG
SexAI 3293 ACCAGGT A^CC[AT]GGT
SgrAI 1977 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1824 CTCAAG C^T[CT][GA]AG
Sse8387I 3643 CCTGCAGG CCTGCA^GG
SspBI 2509 TGTACA T^GTACA
XbaI 4160 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 2619 AAATTT [GA]^AATT[CT]
4864 GAATTT
AhdI 3351 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3719 GACCTGGAGTC
AocI 3208 CCTCAGG CC^T[ACGT]AGG
3554 CCTTAGG
ApoI 2619 AAATTT [GA]^AATT[CT]
4864 GAATTT
AspEI 3351 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3719 GACCTGGAGTC
AviII 574 TGCGCA TGC^GCA
1237 TGCGCA
BbvCI 2847 CCTCAGC CCTCAGC(-5/-2)
2942 GCTGAGG
BciVI 446 GGATAC GTATCC(6/5)
1102 GGATAC
BclI 2657 TGATCA T^GATCA
4103 TGATCA
BlpI 38 GCTCAGC GC^T[ACGT]AGC
2210 GCTGAGC
Bpu1102I 38 GCTCAGC GC^T[ACGT]AGC
2210 GCTGAGC
BsaWI 367 ACCGGA [AT]^CCGG[AT]
1921 ACCGGT
BsrFI 1921 ACCGGT [GA]^CCGG[CT]
1978 ACCGGC
BstBI 509 TTCGAA TT^CGAA
2574 TTCGAA
BstEII 825 GGTCACC G^GT[ACGT]ACC
2254 GGTCACC
BstPI 825 GGTCACC G^GT[ACGT]ACC
2254 GGTCACC
Bsu36I 3208 CCTCAGG CC^T[ACGT]AGG
3554 CCTTAGG
CelII 38 GCTCAGC GC^T[ACGT]AGC
2210 GCTGAGC
Cfr10I 1921 ACCGGT [GA]^CCGG[CT]
1978 ACCGGC
Eam1105I 3351 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3719 GACCTGGAGTC
Eco81I 3208 CCTCAGG CC^T[ACGT]AGG
3554 CCTTAGG
EcoO65I 825 GGTCACC G^GT[ACGT]ACC
2254 GGTCACC
FauI 2876 CCCGC CCCGC(4/6)
2992 GCGGG
FbaI 2657 TGATCA T^GATCA
4103 TGATCA
FspI 574 TGCGCA TGC^GCA
1237 TGCGCA
HgaI 2034 GCGTC GACGC(5/10)
2332 GCGTC
MaeII 1458 ACGT A^CGT
4472 ACGT
NspV 509 TTCGAA TT^CGAA
2574 TTCGAA
PvuII 1253 CAGCTG CAG^CTG
3823 CAGCTG
ScaI 1414 AGTACT AGT^ACT
3473 AGTACT
SfuI 509 TTCGAA TT^CGAA
2574 TTCGAA
SphI 3491 GCATGC GCATG^C
4404 GCATGC
StuI 3620 AGGCCT AGG^CCT
3802 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII