##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AccIII      AlwNI       AocI        
Aor51HI     AscI        Asp718I     AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BlnI        
BseAI       BsiWI       BspDI       BspEI       BspHI       
BsrBI       BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraIII      EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRV       FseI        FspI        HaeII       HindIII     
KasI        KpnI        KspI        MluI        MroI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PshAI       PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SnaBI       Sse8387I    SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1744  GTAGAC               GT^[AC][GT]AC
AclI         2210  AACGTT               AA^CGTT
AcyI            5  GACGCC               G[GA]^CG[CT]C
AflII        4408  CTTAAG               C^TTAAG
AgeI         1068  ACCGGT               A^CCGGT
ApaI         4730  GGGCCC               GGGCC^C
AseI          583  ATTAAT               AT^TAAT
AsnI          583  ATTAAT               AT^TAAT
BciVI        1648  GTATCC               GTATCC(6/5)
BclI          960  TGATCA               T^GATCA
BfrI         4408  CTTAAG               C^TTAAG
BlpI         3032  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     3032  GCTTAGC              GC^T[ACGT]AGC
BsaAI        4969  TACGTG               [CT]AC^GT[GA]
BsaBI        1251  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           5  GACGCC               G[GA]^CG[CT]C
BsaWI        1068  ACCGGT               [AT]^CCGG[AT]
BseRI        1109  CTCCTC               GAGGAG(10/8)
BsiEI        1468  CGACCG               CG[GA][CT]^CG
BsmBI           3  GAGACG               CGTCTC(1/5)
BspLU11I     2372  ACATGT               A^CATGT
BsrFI        1068  ACCGGT               [GA]^CCGG[CT]
BstAPI       2091  GCACATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        3032  GCTTAGC              GC^T[ACGT]AGC
Cfr10I       1068  ACCGGT               [GA]^CCGG[CT]
DrdI          326  GACAGGCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI           37  GCGGG                CCCGC(4/6)
FbaI          960  TGATCA               T^GATCA
HgaI            5  GACGC                GACGC(5/10)
Hin1I           5  GACGCC               G[GA]^CG[CT]C
MamI         1251  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2171  CAATTG               C^AATTG
MspA1I       2390  CAGCTG               C[AC]G^C[GT]G
MunI         2171  CAATTG               C^AATTG
NdeI         2530  CATATG               CA^TATG
PinAI        1068  ACCGGT               A^CCGGT
PpuMI         757  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         583  ATTAAT               AT^TAAT
Psp1406I     2210  AACGTT               AA^CGTT
PvuII        2390  CAGCTG               CAG^CTG
SacI         3317  GAGCTC               GAGCT^C
SapI         4208  GAAGAGC              GCTCTTC(1/4)
ScaI         2366  AGTACT               AGT^ACT
SgrAI        1067  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         3179  CCCGGG               CCC^GGG
SpeI         3966  ACTAGT               A^CTAGT
SphI          145  GCATGC               GCATG^C
StuI         4717  AGGCCT               AGG^CCT
XbaI         4423  TCTAGA               T^CTAGA
XcmI         1464  CCATCGACCGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        3179  CCCGGG               C^CCGGG
XmaI         3179  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflIII       2372  ACATGT               A^C[GA][CT]GT
             4970  ACGTGT              
AhdI         3436  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3724  GACATTTGGTC         
Alw44I       3232  GTGCAC               G^TGCAC
             4476  GTGCAC              
ApaLI        3232  GTGCAC               G^TGCAC
             4476  GTGCAC              
AspEI        3436  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3724  GACATTTGGTC         
AspI           64  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              153  GACACTGTC           
AvaI         3179  CCCGGG               C^[CT]CG[GA]G
             3211  CCCGAG              
BanI         1626  GGCACC               G^G[CT][GA]CC
             1915  GGTGCC              
BbsI         2309  GAAGAC               GAAGAC(2/6)
             4841  GAAGAC              
BglI          801  GCCTACTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4645  GCCATGGAGGC         
BglII        1182  AGATCT               A^GATCT
             1791  AGATCT              
BpuAI        2309  GAAGAC               GAAGAC(2/6)
             4841  GAAGAC              
BsaI           62  GAGACC               GGTCTC(1/5)
             1413  GGTCTC              
BsoBI        3179  CCCGGG               C^[CT]CG[GA]G
             3211  CCCGAG              
BsrDI        2933  CATTGC               GCAATG(2/0)
             4471  GCAATG              
BssHII       4110  GCGCGC               G^CGCGC
             4112  GCGCGC              
BstEII        398  GGTTACC              G^GT[ACGT]ACC
             4747  GGTTACC             
BstPI         398  GGTTACC              G^GT[ACGT]ACC
             4747  GGTTACC             
BstXI        1941  CCAACACATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4269  CCAGCCCCTTGG        
DraII         757  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             4730  GGGCCCT             
DsaI         4175  CCATGG               C^C[GA][CT]GG
             4646  CCATGG              
Eam1105I     3436  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3724  GACATTTGGTC         
EcoO109I      757  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             4730  GGGCCCT             
EcoO65I       398  GGTTACC              G^GT[ACGT]ACC
             4747  GGTTACC             
EcoRI         681  GAATTC               G^AATTC
             2739  GAATTC              
NcoI         4175  CCATGG               C^CATGG
             4646  CCATGG              
PflFI          64  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              153  GACACTGTC           
PflMI        1014  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2005  CCACCCCCTGG         
PleI         3113  GAGTC                GAGTC(4/5)
             4768  GAGTC               
PstI         2640  CTGCAG               CTGCA^G
             4361  CTGCAG              
SexAI        1814  ACCAGGT              A^CC[AT]GGT
             4751  ACCTGGT             
Tth111I        64  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              153  GACACTGTC           
Van91I       1014  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2005  CCACCCCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaII       
BalI        BanII       BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspMI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        EaeI        EarI        EcoRII      
EcoT14I     EcoT22I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaI        HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NsiI        NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI