##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccI AccIII AflII AscI
AseI AsnI Asp718I AviII BamHI
BcgI BciVI BfrI BglII BsaBI
BseAI BsiWI BspEI BssHII Bst1107I
BstBI BstZ17I CpoI DraIII EcoRI
EcoRV EcoT22I FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NsiI NspV
PacI PmeI PshBI PvuI RsrII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 3959 AACGTT AA^CGTT
AgeI 55 ACCGGT A^CCGGT
AocI 3745 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1436 AGCGCT AGC^GCT
Asp700I 2524 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4495 CCTAGG C^CTAGG
BalI 348 TGGCCA TGG^CCA
BbeI 35 GGCGCC GGCGC^C
BclI 2349 TGATCA T^GATCA
BlnI 4495 CCTAGG C^CTAGG
BlpI 479 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 479 GCTCAGC GC^T[ACGT]AGC
BsaI 3508 GAGACC GGTCTC(1/5)
BsmBI 3567 CGTCTC CGTCTC(1/5)
BsmI 2200 GAATGC GAATGC(1/-1)
Bsp1407I 3804 TGTACA T^GTACA
BspDI 2237 ATCGAT AT^CGAT
BspHI 780 TCATGA T^CATGA
BsrGI 3804 TGTACA T^GTACA
BssSI 1634 CACGAG CACGAG(-5/-1)
BstYI 2884 GGATCT [GA]^GATC[CT]
Bsu36I 3745 CCTGAGG CC^T[ACGT]AGG
CelII 479 GCTCAGC GC^T[ACGT]AGC
ClaI 2237 ATCGAT AT^CGAT
DrdI 744 GACCGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1436 AGCGCT AGC^GCT
Eco81I 3745 CCTGAGG CC^T[ACGT]AGG
FbaI 2349 TGATCA T^GATCA
FseI 509 GGCCGGCC GGCCGG^CC
KasI 35 GGCGCC G^GCGCC
MflI 2884 GGATCT [GA]^GATC[CT]
MluNI 348 TGGCCA TGG^CCA
MscI 348 TGGCCA TGG^CCA
NarI 35 GGCGCC GG^CGCC
NotI 1 GCGGCCGC GC^GGCCGC
NruI 50 TCGCGA TCG^CGA
PinAI 55 ACCGGT A^CCGGT
PshAI 3655 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3959 AACGTT AA^CGTT
RcaI 780 TCATGA T^CATGA
SfoI 35 GGCGCC GGC^GCC
SspBI 3804 TGTACA T^GTACA
XbaI 3378 TCTAGA T^CTAGA
XhoII 2884 GGATCT [GA]^GATC[CT]
XmnI 2524 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 1394 GACGTC GACGT^C
3739 GACGTC
AcsI 1924 GAATTT [GA]^AATT[CT]
4539 AAATTC
ApoI 1924 GAATTT [GA]^AATT[CT]
4539 AAATTC
BbrPI 1754 CACGTG CAC^GTG
2245 CACGTG
BbsI 3257 GAAGAC GAAGAC(2/6)
4768 GAAGAC
BbvCI 2602 CCTCAGC CCTCAGC(-5/-2)
2823 CCTCAGC
BpuAI 3257 GAAGAC GAAGAC(2/6)
4768 GAAGAC
BsaWI 55 ACCGGT [AT]^CCGG[AT]
4631 ACCGGA
BspLU11I 2184 ACATGT A^CATGT
4222 ACATGT
BsrDI 4706 CATTGC GCAATG(2/0)
4149 GCAATG
BstAPI 632 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
937 GCAGCGGATGC
BstEII 289 GGTCACC G^GT[ACGT]ACC
2230 GGTCACC
BstPI 289 GGTCACC G^GT[ACGT]ACC
2230 GGTCACC
EcoO65I 289 GGTCACC G^GT[ACGT]ACC
2230 GGTCACC
KspI 627 CCGCGG CCGC^GG
797 CCGCGG
NaeI 10 GCCGGC GCC^GGC
510 GCCGGC
NgoMIV 10 GCCGGC G^CCGGC
510 GCCGGC
PaeR7I 2049 CTCGAG C^TCGAG
2591 CTCGAG
PmaCI 1754 CACGTG CAC^GTG
2245 CACGTG
PmlI 1754 CACGTG CAC^GTG
2245 CACGTG
SacII 627 CCGCGG CCGC^GG
797 CCGCGG
SapI 92 GAAGAGC GCTCTTC(1/4)
127 GAAGAGC
SbfI 706 CCTGCAGG CCTGCA^GG
4241 CCTGCAGG
SfiI 795 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1281 GGCCTCCCAGGCC
Sse8387I 706 CCTGCAGG CCTGCA^GG
4241 CCTGCAGG
SspI 4832 AATATT AAT^ATT
4855 AATATT
XhoI 2049 CTCGAG C^TCGAG
2591 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
ApaI ApaLI AspEI AspHI AspI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XmaCI XmaI